Camphor leaf essential oil-containing plant-sourced pesticide composition and application thereof
A technology of botanical pesticide and composition, which is applied in the field of pesticide application to achieve the effects of increasing action sites, delaying drug resistance, overcoming and drug resistance
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example 1
[0025] Bioassay Example 1 : Indoor Toxicity Determination of Composition of Camphor Leaf Essential Oil and Azadirachtin to Plutella xylostella
[0026] Test object: Cabbage diamondback moth
[0027] The test method is leaf drug film method. Take fresh cabbage leaves without pesticide pollution and soak them in a series of concentrations of camphor tree leaf essential oil and azadirachtin for 10 seconds, and use distilled water as a control, and let them dry indoors. After drying, cut into discs of appropriate size and place them in a 9cm petri dish, insert 3rd instar larvae of the same size, repeat 3-4 times for each concentration, 10-20 larvae for each repetition, and count the mortality after 48 hours , using the least squares method to calculate the LC50 value of the inhibitory concentration. Then calculate the co-toxicity coefficient (CTC) according to Sun Yunpei's method. When CTC120, the composition exhibits synergistic effect.
[0028] Actual toxicity index (ATI) =...
example 2
[0034] Bioassay Example 2 : Indoor Toxicity Determination of Camphor Leaf Essential Oil and Osthole Composition to Tea Geometrid
[0035] Test object: tea inchworm
[0036] The test method is leaf soaking method. Immerse the fresh tea tips in the liquid medicine and dry the cuttings, cut off the dried tea tips and put them in canned bottles, with 3-4 tea tips per bottle, select 2nd instar larvae of the same size and put them into the canned bottle. Each treatment was inoculated with 15 worms, repeated three times, the temperature was controlled at 26-27°C, and the appropriate humidity was maintained, and normal fresh leaves were fed. The mortality rate was investigated 120 hours after administration, and the inhibitory intermediate concentration LC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated by Sun Yunpei's method. When CTC120, the composition exhibits synergistic effect.
[0037] Actual toxicity index (ATI) = (standar...
Embodiment 1
[0046] Camphor leaf essential oil 10%, azadirachtin 1%, N-methylpyrrolidone 4%, Nongru 1601#6%, Tween-60#3%, Nongru2201#4%, epichlorohydrin 7%, benzene Formic acid 2%, propylene glycol 2%, deionized water make up 100%. The 11% camphor leaf essential oil · azadirachtin in water emulsion can be prepared by processing the above components according to the conventional method.
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