Bioluminescence tomography quantitative reconstruction method based on prior interested area of magnetic resonance image

Abstract

The invention relates to a bioluminescence tomography quantitative reconstruction method based on a prior interested area of a magnetic resonance image. The method comprises the specific steps of (1) data collection and preprocessing; (2) reconstruction of an anatomical structure of a living body; (3) acquisition of the prior interested area; (4) mapping of body surface optical data; (5) construction of a forward optical transmission model; (6) establishment of a sparse regularization objective function; (7) optimized solving of the objective function; and (8) display of a three-dimensional reconstruction result. According to the method, anatomical structure information of the living body and the prior interested area for positioning a targeted objective are acquired through the magnetic resonance image, the forward optical transmission model based on a simplified spherical harmonic-diffusion approximation equation and the sparse regularization objective function based on the prior interested area of the magnetic resonance image are constructed, and the in-vivo targeted objective of the complicated living body with multiple kinds of scattering property biological tissue is positioned accurately and quickly and reconstructed quantitatively.

Description

technical field [0001] The invention relates to the technical field of medical imaging (the technical field of optical molecular imaging), in particular to a bioluminescent tomographic quantitative reconstruction method based on a priori region of interest of a magnetic resonance image. Background technique [0002] Bioluminescence imaging is an emerging optical molecular imaging technology, which is a biochemical process in which active molecules or cells emit fluorescence through biochemical reactions, and it adopts a three-dimensional imaging mode. [0003] Bioluminescence tomography technology combines the bioluminescent signal measured on the surface of the organism, the anatomical structure of the organism and the optical parameter information of the tissue, and obtains the three-dimensional spatial position and energy of the target in the living organism based on the accurate light transmission model in the biological tissue distribution information. Because it can p...

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Problems solved by technology

Xidian University, in its patent application document "Optical three-dimensional imaging method based on biological tissue specificity" (application number: 201110148500.6, application date: 2011.06.02, authorization number: ZL201110148500.6, authorization date: 2013.04.03), Also use the micro-computed tomography technology to obtain the anatomical structure information of the organism, fuse the optical characteristic parameters of the biological tissue, and construct the tissue-specific light transmission model, which solves the problem that the existing technology cannot analyze the tissue with irregular anatomical structure and various scattering characteristics. The problem of accurate and fast optical three-dimensional imaging of complex organisms

However, the methods disclosed in these two patents both use microcomputer tomography technology to obtain tissue structure information of organisms. The limitation of microcomputer tomography technology in soft tissue contrast limits the application of such methods in tumor detection, new drug development and curative effect. Appraisal of other small animal experiments

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