Method for detecting number of effective sperms in porcine seminal plasma

Abstract

The invention relates to a method for detecting the number of effective sperms in porcine seminal plasma. The method comprises the following operation steps: 1, arranging a counting cell with 100 small checks on a counting plate, wherein the depth of the counting cell is 0.01mm, and the length and width of the counting cell are respectively 0.1m; 2, preheating the counting plate to 37 DEG C, and covering a piece of cover glass on the counting cell; 3, dripping the to-be-detected porcine seminal plasma between the cover glass and the counting cell, and naturally sucking the porcine seminal plasma into the counting cell; and 4, performing microscope detection, and performing statistical analysis on sperm density, sperm motility and the number of effective sperms. The method disclosed by the invention is easy to operate and suitable for field detection of seminal plasma before use in a pig farm without an automatic semen analyzer, and only simple instruments and components need to be equipped; the calculation method in the invention is simple, and the number obtained by dividing the amount of sperms in a single small check by 10 refers to the sperm density during seminal plasma detection; and moreover, the sperm motility is obtained by dividing the amount of sperms making a linear motion in a single small counting cell by the total number of sperms in the single small counting cell, and the number of effective sperms is obtained by multiplying the sperm motility by the total amount of sperms in the to-be-detected porcine seminal plasma.

Description

technical field [0001] The invention belongs to the technical field of artificial insemination of pigs, and in particular relates to a detection process of effective sperm count in pig semen. Background technique [0002] Pig artificial insemination technology has been tried in the pig industry since the 1920s. After nearly a century of development, the technology has basically matured and is accepted by most pig farms at home and abroad. my country has released the national standards of "Normal Temperature Semen of Breeding Pigs" (GB / T23238-2009) and "Technical Specifications for Production and Storage of Pig Normal Temperature Semen" (GB / T 25172-2010), which stipulate the requirements for normal temperature semen of breeding boars , Sampling, test methods, judgment rules and signs, packaging, storage and transportation, etc., have played an important role in the promotion and implementation of pig artificial technology. With the implementation of the national fine breed su...

AI Technical Summary

Problems solved by technology

However, during the implementation of artificial insemination in boar studs and farms, especially during the transportation of semen, the quality of semen may decline, and even cause the semen to lose its vitality before insemination, and the quality cannot meet the national standard (GB 23238-2009 and GB / T 25172-2010), some expensive testing equipment (sperm automatic analyzer, density meter, etc.) are difficult to be equipped in general pig farms, but room temperature semen still needs to be checked before use, Judging whether it can continue to be used, otherwise it will lead to the failure of mating of sows, prolong the non-production days of sows, and increase the cost of pig farms

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