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High-yield gamma-polyglutamic acid (gamma-PGA) bacterial strain and method for producing gamma-PGA through gamma-PGA bacterial strain

A strain and high-yield technology, applied in the direction of sterilization method, microorganism-based method, support/immobilization method of microorganisms, etc., can solve the problems of complicated process, non-recyclable bacteria, high energy consumption, etc., and achieve simple process, Realize the effect of reusing and improving production efficiency

Active Publication Date: 2017-09-01
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the prior art, free cell fermentation is mostly used for γ-PGA fermentation. Compared with free cells, immobilized cells have the advantages of convenient recovery, repeated use, and short batch production intervals. Therefore, the applicant believes that the SSFF Combining the method with immobilized cell fermentation can effectively solve the problems of complex process, high energy consumption, and non-recyclable fermented bacteria in the production of γ-polyglutamic acid by using lignocellulose at the present stage. The large-scale fermentation of γ-polyglutamic acid as raw material is of great significance

Method used

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  • High-yield gamma-polyglutamic acid (gamma-PGA) bacterial strain and method for producing gamma-PGA through gamma-PGA bacterial strain
  • High-yield gamma-polyglutamic acid (gamma-PGA) bacterial strain and method for producing gamma-PGA through gamma-PGA bacterial strain
  • High-yield gamma-polyglutamic acid (gamma-PGA) bacterial strain and method for producing gamma-PGA through gamma-PGA bacterial strain

Examples

Experimental program
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Embodiment 1

[0042] Production of γ-polyglutamic acid with corn stalks as the main raw material:

[0043] 1) Seed culture: Inoculate the cryopreserved Bacillus amyloliquefaciens JX-12 on the slant of the sterile activation medium, the formula of the activation medium is: peptone 10g, beef extract 5g, NaCl 5g, agar 19g, pH 7.0~ 7.4, add water to 1000mL, and sterilize at 121°C for 30min. Cultivate at 37°C for 24 hours to activate, inoculate the activated strains on sterile seed medium, the formulation of the seed medium is: 10g of peptone, 5g of beef extract, 5g of NaCl, pH 7.0-7.4, replenish water to 1000mL, Sterilize at 121°C for 30min. Shake the flask for 24 hours under the conditions of 30° C. and 200 rpm to obtain the seed liquid.

[0044] 2) Preparation of immobilized cells: centrifuge the seed liquid at 5000 rpm and 4°C for 20 min, discard the supernatant, rinse 3 times with sterile 0.9% saline, suspend the cells with sterile water, and dilute to Concentration is 10 6 cfu / mL.

[...

Embodiment 2

[0058] Production of γ-polyglutamic acid with corn stalks as the main raw material:

[0059] 1) Seed culture: Inoculate the cryopreserved Bacillus amyloliquefaciens JX-12 on the slant of the sterile activation medium, the formula of the activation medium is: peptone 10g, beef extract 5g, NaCl 5g, agar 19g, pH 7.0~ 7.4, add water to 1000mL, and sterilize at 121°C for 30min. Cultivate at 37°C for 24 hours to activate, inoculate the activated strains on sterile seed medium, the formulation of the seed medium is: 10g of peptone, 5g of beef extract, 5g of NaCl, pH 7.0-7.4, replenish water to 1000mL, Sterilize at 121°C for 30 minutes. Shake the flask for 24 hours under the conditions of 30° C. and 200 rpm to obtain the seed liquid.

[0060] 2) Preparation of immobilized cells: centrifuge the seed liquid at 5000 rpm and 4°C for 20 min, discard the supernatant, rinse 3 times with sterile 0.9% saline, suspend the cells with sterile water, and dilute to Concentration is 10 6 cfu / mL....

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Abstract

The invention belongs to the field of microbial fermentation engineering and aims at providing high-yield gamma-polyglutamic acid (gamma-PGA) bacillus sp. JX-12 and providing application of the high-yield gamma-PGA bacterial strain in producing gamma-polyglutamic acid. A preservation number of the bacterial strain JX-12 in China General Microbiological Culture Collection Center (CGMCC) is CGMCCNO.13716. The high-yield gamma-PGA bacterial strain disclosed by the invention adopted the technical scheme that lignocellulose is utilized as a main raw material to product gamma-polyglutamic acid, and the method comprises the steps of cultivating seeds, preparing immobilized cells, pretreating and synchronously saccharifying the lignocellulose materials and filtering and fermenting. The high-yield gamma-PGA bacterial strain disclosed by the invention has the advantages of low production cost, simple technology and high production efficiency; continuous batch fermentation is achieved; important significance in industrial expanding of utilizing the lignocellulose as a carbon source to product gamma-polyglutamic acid is realized.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and in particular relates to a gamma-polyglutamic acid producing bacterium and a process of synchronous saccharification, filtration and fermentation. Background technique [0002] γ-polyglutamic acid (English name γ-polyglutamic acid, γ-PGA for short) is composed of L-glutamic acid or D-glutamic acid through the amide bond between α-amino group and γ-carboxyl group. γ-polyglutamic acid and its derivatives have been widely used in drug carriers , water treatment flocculants, agricultural water and fertilizer retention agents, food and cosmetic additives and other fields. In the traditional process, γ-polyglutamic acid is mainly prepared by microbial fermentation. Glucose, glycerol and citric acid are the main carbon sources for the fermentation of γ-polyglutamic acid, but these carbon source nutrients are all derived from human food. Such as grain, potatoes, sugar cane, etc., w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/02C12N11/10C12N11/08C12M1/40C12M1/12C12M1/02C12R1/07
CPCC12M21/18C12M27/02C12M29/04C12M29/18C12M37/02C12M45/09C12N11/08C12N11/10C12P13/02C12P2201/00C12P2203/00C12N1/205C12R2001/07
Inventor 闫志英姬高升许力山房俊楠刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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