Active agent for promoting ganoderma lucidum to grow
An active agent, Ganoderma lucidum technology, applied in the field of active agent to promote the growth of Ganoderma lucidum, can solve the problems of injury, legacy, residual toxic and harmful substances, etc., and achieve the effect of low cost, simple process steps, and strong operability
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Embodiment 1
[0024] (1) Culture medium preparation
[0025] Select 200g of potatoes, 20g of agar, and 20g of glucose. The above materials are used to make a basal medium and put into an Erlenmeyer flask. Weigh 650g of sodium diacetate, 100g of potassium sorbate, 280g of vitamin C, and 80g of sodium benzoate, dilute with water to 1000L. When active agents are used, 7 mL of diluted active agents are added to each liter of culture solution to cultivate Ganoderma lucidum strains.
[0026] (2) Medium sterilization
[0027] Put the prepared cultivation baskets into an autoclave and sterilize at 121°C for 30 minutes.
[0028] (3) Vaccination
[0029] When the temperature of the culture medium drops to 36-40°C, one group transfers Ganoderma lucidum to the culture medium added with active agent, which is designated as group A, and the other group transfers Neurospora species to the culture medium added with active agent Base, marked as group B, sealed with a parafilm after inoculation, and plac...
Embodiment 2
[0033] 1) Culture medium preparation
[0034] Select 200g of potatoes, 20g of agar, and 20g of glucose. The above materials are used to make a basal medium and put into an Erlenmeyer flask. Weigh 700g of sodium diacetate, 120g of potassium sorbate, 280g of vitamin C, and 80g of sodium benzoate, dilute with water to 1000L, and add 7mL of diluted active agent per liter of culture solution to cultivate Ganoderma lucidum strains.
[0035] (2) Medium sterilization
[0036] Put the prepared cultivation baskets into an autoclave and sterilize at 121°C for 30 minutes.
[0037] (3) Inoculation
[0038] When the temperature of the culture medium drops to 36-40°C, transfer the Ganoderma lucidum to the culture medium added with the active agent.
Embodiment 3
[0040] 1) Culture medium preparation
[0041] Select 200g of potatoes, 20g of agar, and 20g of glucose. The above materials are used to make a basal medium and put into an Erlenmeyer flask. Weigh 700g of sodium diacetate, 120g of potassium sorbate, 300g of vitamin C, and 80g of sodium benzoate, dilute with water to 1000L, and add 7mL of diluted active agent per liter of culture solution when using active agent to cultivate Ganoderma lucidum strains.
[0042] (2) Medium sterilization
[0043] Put the prepared cultivation baskets into an autoclave and sterilize at 121°C for 30 minutes.
[0044] (3) Inoculation
[0045] When the temperature of the culture medium drops to 36-40°C, transfer the Ganoderma lucidum to the culture medium added with the active agent.
[0046] Table 1 Effects of different concentrations of active agents on the growth of Ganoderma lucidum mycelium (long 5 days)
[0047]
[0048] Taking no active agent as blank control, it was found that the active ...
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