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Trichoderma asperellum DQ3 compound powder YBY for rice breeding and application

A technology of Trichoderma aculeatus and composite powder, which is applied in Trichoderma aculeatus DQ3 composite powder YBY and its application field, which can solve the problems of human and livestock toxicity, environmental pollution, residue, etc., and achieve the effect of improving soil and promoting root growth

Inactive Publication Date: 2017-09-01
刘铜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] An object of the present invention is to provide the Trichoderma aculeatus DQ3 composite powder YBY that is used for rice seedling raising, and this Trichoderma aculeatus DQ3 composite powder YBY that is used for rice seedling cultivation solves the problem of the current use of chemical pesticides in the prevention and treatment of rice root diseases and Soil-borne diseases are toxic to humans and animals, pollute the environment, and have residual problems. At the same time, they can promote the development of rice roots and improve stress resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This Trichoderma aculeatus DQ3 composite powder YBY used for rice seedlings is prepared by the following method:

[0022] Step 1: Inoculate Trichoderma aculeatus DQ3 strain on PDA slant medium, culture at 28°C for 72 hours, wash the conidia with sterilized distilled water to make spore liquid, the number of spores in the spore liquid is 10 6 1 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28℃, 120rpm and cultivate for 36h;

[0023] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 minutes. After cooling, put it into a plastic bottle according to the inoculation amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 6 days; the fermented bacteria in the plastic ...

Embodiment 2

[0029] This Trichoderma aculeatus DQ3 composite powder YBY used for rice seedlings is prepared by the following method:

[0030] Step 1: Inoculate Trichoderma aculeatus DQ3 strain on PDA slant medium, culture at 28°C for 96 h, wash the conidia with sterilized distilled water, and make spore liquid, the number of spores in the spore liquid is 10 6 1 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28°C, 120rpm for 40h;

[0031] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 min. After cooling, put it into a plastic bottle according to the inoculum amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 7 days; the fermented bacteria in the plastic bottle Seeds are adde...

Embodiment 3

[0036] This Trichoderma aculeatus DQ3 composite powder YBY used for rice seedlings is prepared by the following method:

[0037]Step 1: Inoculate Trichoderma aculeatus DQ3 strain on PDA slant medium, culture at 28°C for 84 h, wash the conidia with sterilized distilled water, and make spore liquid, the number of spores in the spore liquid is 10 6 1 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28°C, 120rpm for 48h;

[0038] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 min. After cooling, put it into a plastic bottle according to the inoculum amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 7 days; the fermented bacteria in the plastic bottle Seeds are added...

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PUM

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Abstract

The invention relates to trichoderma asperellum DQ3 compound powder YBY for rice breeding and application. The trichoderma asperellum DQ3 compound powder YBY for rice breeding is prepared according to the following steps of: firstly, inoculating trichoderma asperellum DQ3 strains in a PDA agarslant culture-medium and cultivating so as to prepare a spore solution, and inoculating the spore solution in a sterilizing PD culture solution and cultivating; second, mixing wheat bran, diatomite, rice husk and water according to a mass ratio of 2:1:1:4 so as to prepare a spore suspension to be used as a solid shallow tray fermentation seed solution; third, stirring a solid shallow tray fermentation substrate with wheat bran, diatomite, molasses water and sterile water, then packing with plastic bags and sterilizing; mixing the sterilized fermentation substrate and the solid shallow tray fermentation seed solution, cultivating, and smashing an air dried shallow tray fermentation product so as to obtain trichoderma asperellum DQ3 spore powder; and fourth, preparing the trichoderma asperellum DQ3 compound powder YBY for rice breeding. The trichoderma asperellum DQ3 compound powder YBY for rice breeding is used for promoting rice root growing and resisting disease and drought.

Description

[0001] 1. Technical field [0002] The invention relates to a biological bacterial agent for preventing and controlling crop diseases, in particular to Trichoderma aculeatus DQ3 composite powder YBY used for rice seedling raising and its application. [0003] 2. Background technology [0004] Rice is native to China and is one of the most important food crops for human beings. With the continuous growth of our country's population and the aggravation of the shortage of land resources, increasing rice production plays a very important role in solving the problem of our country's food self-sufficiency. Therefore, rice production technology has always been a research hotspot. A folk proverb says: "Half of the seedlings are good", which shows that strong rice seedlings are the basis for increasing production. To achieve high yields of rice and maximize the advantages of increasing production, strong seedlings should be cultivated. With the advancement of rice cultivation technolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01N43/16A01N25/14A01P3/00A01P21/00C12N1/14C12R1/885
CPCA01N25/14A01N43/16A01N63/00A01N63/30C12N1/14
Inventor 刘铜侯巨梅
Owner 刘铜
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