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Fully-nutritional water-soluble microbial polypeptide bacterial fertilizer and production method thereof

A microbial and full-nutrition technology, applied in the field of full-nutrition water-soluble microbial polypeptide bacteria, can solve the problems of few full-nutrition water-soluble fertilizers, single water-soluble fertilizer formula, and the diversity of water-soluble fertilizer products needs to be improved, so as to achieve easy use , good water solubility

Inactive Publication Date: 2017-08-29
中航立兴(北京)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of March 2, 2015, the Ministry of Agriculture has registered 6,547 water-soluble fertilizer products. Most of the water-soluble fertilizers have a single formula, and there are very few water-soluble fertilizers with full nutrition. The diversity of water-soluble fertilizer products needs to be improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 full nutrition water-soluble microbial polypeptide inoculum preparation

[0023] 1). Slant culture: Trichoderma harzianum Q2, Saccharomyces cerevisiae T6, and Bacillus cerevisiae J5 were inoculated on solid medium under sterile conditions, and Trichoderma harzianum Q2 and Saccharomyces cerevisiae T6 were cultured at 30°C for 60 -84 hours; Bacillus lateralis J5, cultured at 37°C for 48-60 hours;

[0024] 2). First-level seed cultivation: Inoculate the strains cultivated in step 1) into liquid medium under aseptic conditions, Trichoderma harzianum Q2 and Saccharomyces cerevisiae T6 were cultured at 30°C for 60-84 hours, and Bacillus lateralis J5 was cultured at 37°C for 48-60 hours to obtain first-class seeds, and the optical density OD600 values ​​of each Saccharomyces cerevisiae and Bacillus amyloliquefaciens suspensions reached 3.0 and 2.5, respectively;

[0025] 3).Secondary seed culture: Inoculate the primary seeds into 70L fermenters with the volume ra...

Embodiment 2

[0032] Embodiment two field demonstration test

[0033] In this water-soluble fertilizer field demonstration test, the test site is located in Shandong, with a mild climate and abundant rainfall. The area of ​​the selected vegetable greenhouse is 2 mu. There are two treatments: conventional fertilization, and full-nutrition water-soluble microbial polypeptide bacteria agent, each treatment is 1 mu. The specific method of use of the full-nutrition water-soluble microbial polypeptide bacterial agent is: dilute it with 1:500-800 times of water, and apply it with water.

[0034] The results show that: with conventional fertilization, the yield per mu of cucumbers is 15,000 kg, while the yield per mu of cucumbers can reach 19,000 kg with the application of full-nutrient water-soluble microbial polypeptide bacteria agent, an increase of 4,000 kg, and a yield increase rate of 26.67%.

Embodiment 3

[0035] Example 3 Demonstration of controlling root-knot nematode in the field

[0036] The nematode control experiment of the complete nutrient water-soluble microbial polypeptide agent is located in Shouguang City, Shandong Province. Tomatoes have been cropped continuously for many years, and root-knot nematode disease is serious. In this experiment, 3 treatments were set up, namely Abamectin positive treatment, CK negative treatment, and full nutrient water-soluble microbial polypeptide bacterial agent treatment. Each treatment was repeated 3 times, and the plots were randomly distributed. The use of abamectin is to irrigate the roots with a 2000-fold dilution, and the specific use of the full-nutrition water-soluble microbial polypeptide bacterial agent is: dilute it with 1:500-800 times of water, and apply it with water.

[0037] The results showed that: compared with the blank control, the root-knot index decreased by 75% compared with the blank control, and the tomato yi...

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Abstract

The invention relates to a water-soluble microbial polypeptide bacterial fertilizer, and a production method and application thereof. The bacterial fertilizer comprises trichoderma harzianum Q2, saccharomyces cerevisiae T6 and bacillus laterosporus laubach J5. Optimal proportioning and optimal production of compound bacteria are realized through slant cultivation, first-level seeds, second-level sees as well as mixed cultivation and fermentation, and the living bacteria count of the bacteria is more than or equal to 2.5*10<9> cfu / mL. The compound microbial preparation provided by the invention is rich in active substances such as microorganisms, polypeptide, organic materials, nitrogen phosphorus potassium, amino acid chelated medium and trace elements and biochemical fulvic acid, and has remarkable effects of having full nutrition, promoting growth, resisting diseases, detoxifying, activating soil, resisting continuous cropping and the like.

Description

technical field [0001] The invention relates to a full-nutrition water-soluble microbial polypeptide bacterial agent, in particular to a microbial polypeptide bacterial fertilizer which has the functions of full nutrition, disease resistance, growth promotion, detoxification, and insecticide, and is used to resist heavy cropping and overcome continuous cropping obstacles. The invention also relates to a production method and application of the compound microbial fertilizer. technical background [0002] Facility agriculture is an important form of modern agriculture. It is an effective way to build modern agriculture, prosper the rural economy, and increase farmers' income. As a modern agricultural production method, facility agriculture integrates bioengineering and environmental technology. It has high-tech, With the characteristics of high output and high input, the development of facility agriculture is an important way to improve the efficiency of agricultural production...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/18C05G3/00C05G3/02C12R1/885C12R1/865C12R1/07C05G3/60
CPCC05C11/00C05G3/00C05G3/60C12N1/14C12N1/18C12N1/20C05F11/00C05F11/08
Inventor 黄忠诚
Owner 中航立兴(北京)科技发展有限公司
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