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Method for preparing ecological bionic microbial flocculant from leftovers of marine products

A technology for marine product scraps and microbial flocculants, which is applied in chemical instruments and methods, flocculation/sedimentation water/sewage treatment, water/sewage treatment, etc., can solve the problem of high cost of culture medium, achieve good flocculation effect and reduce costs Effect

Inactive Publication Date: 2017-08-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After research in our laboratory, we found that an ecological biomimetic microbial flocculant can be produced, but due to the high cost of the medium, further improvements have been made

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for preparing an ecological biomimetic microbial flocculant using leftovers from seafood includes the following steps:

[0033] ⑴ Pick out the impurities in the purchased seafood scraps (fish belly, fish meat scraps), wash the seafood scraps 1-2 times with tap water, and then wash 2-3 times with distilled water; then remove the large parts After cutting and crushing, weigh about 500g into a 1000mL beaker; add 200mL of artificial seawater;

[0034] ⑵Add 200mL of concentrated hydrochloric acid (37%); keep it at 95℃ for 24h to hydrolyze;

[0035] (3) Filter with four layers of gauze, collect the filtrate and neutralize it with sodium hydroxide solution to obtain the original hydrolysis solution. Collect the stock solution and dilute to 500mL with artificial sea water;

[0036] ⑷Use the instrument to measure the carbon content, nitrogen content, and phosphorus content. Since the C / N ratio in the unmodified medium was between 22:1 and 23:1, add 1g of glucose and 0.5g of p...

Embodiment 2

[0042] A method for preparing an ecological biomimetic microbial flocculant using leftovers from seafood includes the following steps:

[0043] ⑴ Pick out the impurities in the purchased seafood scraps (fish belly, fish meat scraps), wash the seafood scraps 1-2 times with tap water, and then wash 2-3 times with distilled water; then remove the large parts After cutting and crushing, weigh about 500g into a 1000mL beaker; add 400mL of artificial seawater;

[0044] ⑵Add 400mL of concentrated hydrochloric acid (37%); keep it at 115℃ for 12h to hydrolyze;

[0045] (3) Filter with four layers of gauze, collect the filtrate and neutralize it with sodium hydroxide solution to obtain the original hydrolysis solution. Collect the stock solution and dilute to 1000 mL with artificial sea water;

[0046] ⑷Use the instrument to measure the carbon content, nitrogen content, and phosphorus content. Since the C / N ratio in the unmodified medium is between 22:1 and 23:1, add 3g glucose and 1g potassi...

Embodiment 3

[0052] A method for preparing an ecological biomimetic microbial flocculant using leftovers from seafood includes the following steps:

[0053] ⑴ Pick out the impurities in the purchased seafood scraps (fish belly, fish meat scraps), wash the seafood scraps 1-2 times with tap water, and wash 2-3 times with distilled water; then cut the large parts After cutting and roughly grinding, weigh about 500g into a 1000mL beaker; add 200mL of artificial seawater;

[0054] (2) Weigh 1g of papain and neutral protease into a beaker and adjust the pH to around 6-7; adjust the temperature to 40℃ in a water bath and keep it warm for 4 hours;

[0055] (3) Filter with four layers of gauze, collect the filtrate and neutralize it with sodium hydroxide solution to obtain the enzymatic hydrolysis stock solution. Collect the stock solution and dilute to 500 mL with artificial sea water.

[0056] ⑷Use the instrument to measure the carbon content, nitrogen content, and phosphorus content. Since the C / N rat...

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PUM

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Abstract

The invention discloses a method for preparing an ecological bionic microbial flocculant from leftovers of marine products. The method comprises the following steps: inoculating sludge suspension spat out by ruditapes philippinarum into a liquid culture base prepared from the leftovers of marine products and culturing, and then naturally settling or centrifugally separating precipitates and freeze-drying. According to the invention, the cost of the culture medium can be greatly reduced, the flocculation effect is excellent, pollutants and suspension particles in water can be effectively removed and the excellent settling property is guaranteed.

Description

Technical field [0001] The invention relates to a method for producing a microbial flocculant, in particular to a method for preparing an ecological bionic microbial flocculant from leftovers of seafood. Background technique [0002] Flocculants have extremely wide and critical applications in the fields of water supply treatment, sewage purification, food and fermentation engineering. According to molecular composition, flocculants can be divided into three categories: inorganic flocculants, organic synthetic polymer flocculants and natural polymer flocculants. Inorganic flocculants are cheap, but they have adverse effects on human health and the ecological environment; organic synthetic polymer flocculants such as polyacrylamide, whose monomer acrylamide has strong carcinogenic and neurotoxicity to humans; polyacrylamide is also difficult to degrade , Easy to accumulate in the environment and cause secondary pollution. Natural polymer flocculants from plants and animals often...

Claims

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Application Information

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IPC IPC(8): C02F1/52
CPCC02F1/5263
Inventor 穆军王东方穆思源邢宇崔霞
Owner ZHEJIANG OCEAN UNIV
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