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A Strain of Pediococcus lactis bcc-1 Using Highly Efficient Xylo-oligosaccharides and Its Application

A technology of Pediococcus lactis and BCC-1, which is applied in the field of livestock and poultry breeding and feed preparation, can solve the problems of lack of Pediococcus lactis and less of Pediococcus lactis, and achieve the effects of increasing content, reducing viscosity and improving production performance

Active Publication Date: 2020-11-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the application of Pediococcus lactis in livestock and poultry, especially in broiler chickens, and there is still a lack of relevant reports

Method used

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  • A Strain of Pediococcus lactis bcc-1 Using Highly Efficient Xylo-oligosaccharides and Its Application
  • A Strain of Pediococcus lactis bcc-1 Using Highly Efficient Xylo-oligosaccharides and Its Application
  • A Strain of Pediococcus lactis bcc-1 Using Highly Efficient Xylo-oligosaccharides and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Isolation, screening, identification and preservation of Pediococcus lactis BCC-1

[0049] 1. Isolation and purification of Pediococcus lactis: under aseptic conditions, chyme was isolated from the cecum of healthy white-feathered broiler chickens (42 days old) fed without antibiotic feed, and agar MRS medium was used to treat the chyme supernatant (suitable dilution) to cultivate, select a single lactic acid bacteria colony to purify, and each colony is purified for 10 generations.

[0050] 2. Observation of the colony morphology: The colonies on the agar MRS plate are milky white, round, with neat edges, indicating that they are moist and smooth, and easy to stir up.

[0051] 3. Screening of excellent Pediococcus lactis: XOS was used to replace the glucose in the MRS medium, the xylose and glucose treatment group was used as a positive control, and no sugar source was added as a negative control, and the OD of the bacterial solution was measured after 24 hou...

Embodiment 2

[0057] A kind of probiotics, its active ingredient comprises Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and XOS (xylo-oligosaccharide); Wherein the content of Pediococcus acidilactici BCC-1 is 1 * 1010cfu / g; The mass fraction of XOS is 45%.

[0058] The present embodiment also provides the preparation method of this probiotics, comprising the following steps:

[0059] 1) After preparing the seed liquid of Pediococcus lactis BCC-1 with MRS medium, inoculate according to the inoculum amount of 1% to carry out liquid fermentation culture.

[0060] 2) The medium used in the fermentation broth is MRS medium, the fermentation condition is 30°C, the rotation speed is 200rpm, the pH is adjusted to 6.5 with ammonia water, and the anaerobic fermentation is carried out for 24 hours. % sucrose solution.

[0061] 3) Accurately weigh and ferment 1L of lactic acid bacteria fermentation broth, add 300g of starch to it, and after spray drying, the number of active lactic acid ...

experiment example 1

[0065] The Pediococcus lactis BCC-1 used in the present invention is isolated from cecal digesta of white-feathered broiler chickens. During in vitro fermentation, after entering the stationary phase, the OD600 of the bacterial liquid when BCC-1 uses XOS to proliferate is greater than that of glucose (Glucose), fructooligosaccharide (FOS) and xylose (Xylose), so XOS has a better effect on BCC-1. Proliferation effect. See the experimental results figure 2 . Depend on figure 2 It can be seen that in the absence of carbon source, Pediococcus lactis can hardly grow (Control). FOS had a weak growth-promoting effect, and the bacteria fell into decline and died after 6 hours of growth, indicating that Pediococcus lactis had a weak ability to utilize FOS. Xylose showed a better growth-promoting effect on Pediococcus lactis, but its effect was slightly weaker than that of glucose. The growth-promoting effect of XOS on lactic acid bacteria is stronger than that of xylose. Althoug...

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Abstract

The invention relates to a strain of Pediococcus lactis BCC‑1 that efficiently utilizes xylooligosaccharides and its application. The invention aims at intestinal health problems that are prone to occur in the modern livestock and poultry production process, and provides a strain of Pediococcus lactis BCC‑1 that efficiently utilizes xylooligosaccharides and its application; Feed additive composed of BCC‑1 and xylooligosaccharides can further improve the application effect of lactic acid bacteria, improve the intestinal tissue morphology of the poultry foregut, increase the content of short-chain fatty acids in the cecum, and reduce the pH value of the cecum to improve poultry’s health Intestinal health greatly improves the production performance of poultry and realizes green and healthy farming.

Description

technical field [0001] The invention belongs to the technical field of livestock and poultry breeding and feed preparation, and in particular relates to a strain of Pediococcus acidilactici (Pediococcus acidilactici) BCC-1 and an application thereof. Background technique [0002] my country is a large livestock and poultry breeding country. With the development of my country's breeding industry, a large amount of compound feed needs to be consumed every year. In 2016, my country's feed production reached 19,000 tons. Feed materials Corn, wheat and other energy feeds contain a large amount of anti-nutritional factor xylan (arabinoxylan, AX). After xylan enters the livestock and poultry body, it will form viscous chyme, which will lead to intestinal health problems such as the proliferation of pathogenic bacteria and intestinal inflammation, reduce the utilization of nutrients, and then reduce the production performance of livestock and poultry. Therefore, eliminating the ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 袁建敏雷钊呙于明
Owner CHINA AGRI UNIV
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