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A kind of in vitro rapid propagation method of L. latifolia

A wide-leafed solitary vegetable and solitary vegetable technology, applied in the field of plant tissue culture, can solve problems such as low reproduction efficiency, achieve the effects of improving reproduction efficiency, overcoming geographical restrictions, and expanding the source range

Active Publication Date: 2018-01-23
QILU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention overcomes the problem of low reproduction efficiency of waffles in eastern my country, and greatly promotes the cultivation and popularization of waffles

Method used

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  • A kind of in vitro rapid propagation method of L. latifolia
  • A kind of in vitro rapid propagation method of L. latifolia
  • A kind of in vitro rapid propagation method of L. latifolia

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] (1) Preparation of sterile explants: select mature and plump Lepidium chinensis seeds, sterilize them with a mass concentration of 2% sodium hypochlorite for 25 minutes, wash them with sterile water for 4 times, and sow them on MS basic medium. ~ 14 days old seedlings of L. japonica seedlings are chopped whole and used as sterile explants for rapid propagation in vitro.

[0034] (2) Inoculate the sterile explants on the rapid propagation medium to induce the regenerated seedlings and rapidly amplify and propagate; the conditions used for the rapid amplify and propagate are 23-25°C for cultivation, 14 hours of light per day, and 10 hours of light In the dark, subculture once every 14-21 days, and induce culture for 28-42 days.

[0035] (3) Transfer the induced regenerated shoots into MS basic medium for rooting induction; the conditions for rooting induction are: culture at 23-25° C., 14 hours of light and 10 hours of darkness per day, and co-induction culture for 20 day...

Embodiment 1

[0043] Embodiment 1: Gather in Qinghai the normal seedling rapid propagation of L. latifolia

[0044] The specific method is as follows:

[0045] (1) Acquisition of sterile explants: select mature and plump ones from the seeds of L. latifolia harvested in Qinghai area, sterilize them with 2% sodium hypochlorite for 25 minutes, wash them with sterile water for 4 times, and sow them in MS basic culture. Basically, 10 days after the germination of the sterile seedlings, the whole seedlings were evenly cut into 10 pieces as sterile explants for rapid propagation in vitro.

[0046] (2) Inoculate the sterile explants on the rapid propagation medium, the culture conditions are 23-25 ​​°C, 14 hours of light every day, 10 hours of darkness, subculture once every 14 days, co-induction culture for 28 days, and obtain in vitro expansion. Prosperous regenerated L. latifolia in vitro seedlings, the average number of each explant forming in vitro regenerated seedlings is 1.104 strains in th...

Embodiment 2

[0051] Example 2: Rapid propagation of radicle-deficient seedlings of L. latifoliatum harvested in Shandong

[0052] The specific method is as follows:

[0053] (1) Acquisition of sterile explants: select mature and plump ones from the seeds of L. latifolia harvested in Shandong, sterilize them with 2% sodium hypochlorite for 25 minutes, wash them with sterile water for 4 times, and sow them in MS basic culture. Basically, 14 days after the germination of the sterile seedlings, the whole seedlings lacking radicle were evenly cut into 10 pieces as sterile explants for rapid propagation in vitro.

[0054] (2) Inoculate the sterile explants on the rapid propagation medium, the culture conditions are 23-25 ​​° C, 14 hours of light every day, 10 hours of darkness, subculture once every 21 days, co-induce and culture for 42 days, and obtain in vitro expansion. Prosperous regenerated L. latifolia in vitro seedlings, the average number of each explant forming in vitro regenerated see...

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Abstract

The invention discloses an in-vitro rapid propagation method of wide-blade garden cress. The propagation method comprises the following steps of preparing aseptic explants of the wide-blade garden cress; inoculating the aseptic explants in a fast propagation medium, and conducting induction and rapid amplifying propagation on regrowths; transferring the obtained regrowths after induction to a MS minimal medium for root induction; transplanting the whole regenerated plants of the rooting garden cress to a cultivating medium for normal growth. The propagation method is the first for in-vitro rapid propagation of the wide-blade garden cress at home and abroad, effectively increases propagation coefficients of the wide-blade garden cress, and solves the problem that amplifying propagation of wide-blade garden cress seeds is geographically limited.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to an in vitro rapid propagation method of L. latifolia. Background technique [0002] Lipea broadleaf is a perennial herb, distributed in southern Europe, northern Africa, western and central Asia at an altitude of 1,800 to 4,250 meters. The whole herb of L. latifolia is used as medicine. Traditional Chinese medicine believes that it can cure bacillary dysentery and enteritis. Its seeds contain sitosterol and cardiac glycosides, which can strengthen the heart contraction and slow down the heart rate. venous pressure. [0003] According to the records of Flora of China, Lepidium latifolium L.var.latifolium is divided into Lepidium latifolium L.var.latifolium native to Qinghai and Lepidium latifolium L.var.latifolium native to North China. Lepidium latifolium L.var.affine. With the continuous development of the edibility of Lepidium latifolium, people have gradually d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李现禹蒋苏赵淑举曹子谊李师鹏褚晓张焕香
Owner QILU NORMAL UNIV
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