Tumor marker related to colorectal cancer and application
A tumor marker, colorectal cancer technology, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. effect of meaning
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[0017] The kit for auxiliary diagnosis, curative effect prediction and prognosis judgment of colorectal cancer includes:
[0018] (1) Reagents for extracting total RNA from colorectal cancer and cancer disc tissues, including Trizol reagent, chloroform, isopropanol, and DEPC water.
[0019] (2) Reagents used for reverse transcription of total RNA into cDNA: promega reverse transcription kit (GoScript TM Reverse Transcriptase, A5003), including: GoScript TM 5X Reaction Buffer, MgCl 2 , PCR NucleotideMix, Recombinant Ribonuclease Inhibitor, GoScript TM Reverse Transcriptase, Nuclease-Free Water.
[0020] (3) Reagents used for cDNA real-time fluorescent quantitative PCR, including lncRNA-KAT7 real-time fluorescent quantitative PCR specific primers, GAPDH internal reference specific primers, real-time fluorescent quantitative SYBR dye (Roche 4887352001 LightCycler480 SYBR Green I Master), DEPC water.
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