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Fluorescent copper nano-particles for polyadenylic purine DNA template, as well as preparation method and application thereof

A polyadenine and nanoparticle technology, applied in the fields of material science and analytical chemistry, to achieve the effects of simple experimental operation, high sensitivity, and improved sensitivity and detection limit

Inactive Publication Date: 2017-08-11
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the use of polyadenine DNA as a template to synthesize fluorescent copper nanoparticles. Therefore, the study of using polyadenine DNA as a template to synthesize fluorescent copper nanoparticles and use them in biochemical detection has important practical significance.

Method used

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  • Fluorescent copper nano-particles for polyadenylic purine DNA template, as well as preparation method and application thereof
  • Fluorescent copper nano-particles for polyadenylic purine DNA template, as well as preparation method and application thereof
  • Fluorescent copper nano-particles for polyadenylic purine DNA template, as well as preparation method and application thereof

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preparation example Construction

[0025] The preparation method of the fluorescent copper nanoparticles of the polyadenine DNA template comprises the following steps:

[0026] (1) Design polyadenine DNA probes;

[0027] (2) Dilute the probe prepared in step (1) to 1-10 μmol / L with triple distilled water;

[0028] (3) Dilute the solution obtained in step (2) into MOPS buffer system, add 1-5 mmol / L ascorbic acid solution and mix well;

[0029] (4) Add 0.1-0.8 mmol / L copper sulfate solution to the solution obtained in step (3), and react at room temperature for 1-10 min to obtain fluorescent copper nanoparticles.

[0030] The number of adenine bases in the probe is not less than 20; the amount of the probe is 1-10 μmol / L;

[0031] The dosage of the ascorbic acid solution is 1-5 mmol / L; the dosage of the copper sulfate solution is 0.1-0.8 mmol / L;

[0032] The synthesis time of the step (3) fluorescent copper nanoparticles is 1-10 min;

[0033] The fluorescent copper nanoparticles of the polyadenine DNA templat...

Embodiment 1

[0035] The method for preparing the fluorescent copper nanoparticles of the polyadenine DNA template of the present invention uses the polyadenylation DNA as a synthetic template, uses ascorbic acid as a reducing agent, and prepares copper nanoparticles with fluorescent properties at room temperature.

[0036] A method for preparing fluorescent copper nanoparticles of a polyadenine DNA template, comprising the following steps:

[0037] (1) Design polyadenine DNA probes;

[0038] (2) Dilute the probe prepared in step (1) to 1 μmol / L with triple distilled water;

[0039] (3) Dilute the solution obtained in step (2) into MOPS buffer system, add 1 mmol / L ascorbic acid solution and mix well;

[0040] (4) Add 0.1 mmol / L copper sulfate solution to the solution obtained in step (3), and react at room temperature for 2 min to obtain fluorescent copper nanoparticles.

Embodiment 2

[0042] The method for preparing the fluorescent copper nanoparticles of the polyadenine DNA template of the present invention uses the polyadenylation DNA as a synthetic template, uses ascorbic acid as a reducing agent, and prepares copper nanoparticles with fluorescent properties at room temperature.

[0043] A method for preparing fluorescent copper nanoparticles of a polyadenine DNA template, comprising the following steps:

[0044] (1) Design polyadenine DNA probes;

[0045] (2) Dilute the probe prepared in step (1) to 5 μmol / L with triple distilled water;

[0046] (3) Dilute the solution obtained in step (2) into MOPS buffer system, add 3 mmol / L ascorbic acid solution and mix well;

[0047] (4) Add 0.5 mmol / L copper sulfate solution to the solution obtained in step (3), and react at room temperature for 5 min to obtain fluorescent copper nanoparticles.

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Abstract

The invention discloses a novel method for preparing fluorescent copper nano-particles for polyadenylic purine DNA template, as well as a preparation method and application thereof. The preparation method comprises the following steps: adding a copper sulfate aqueous solution into a 3-(N-morpholine) propanesulfonic acid (MOPS) buffer solution containing polyadenylic purine DNA and ascorbic acid by using the polyadenylic purine DNA as a template and ascorbic acid as a reducing agent to prepare copper nano-particles with fluorescent property at normal temperature. The preparation method is simple and quick and has low cost, and the prepared fluorescent copper nano-particles have the advantages of low toxicity, good stability, strong fluorescent signal, good biocompatibility and the like; and the fluorescent copper nano-particles show potential application in the aspect of biochemical analysis due to the wide source of copper. By utilizing the fluorescence quenching effect of p-benzoquinone for the copper nano-particles, high-sensitive detection on the p-benzoquinone can be realized, and the detection limit can reach 1*10<-10>mol / L.

Description

technical field [0001] The invention relates to the fields of material science and analytical chemistry, in particular to a fluorescent copper nanoparticle of a polyadenine DNA template and a preparation method and application thereof. Background technique [0002] P-benzoquinone, the chemical name is 1,4-benzoquinone or 1,4-p-benzoquinone, also known as benzoquinone. As a dye intermediate, it is often used in the pharmaceutical and chemical fiber industries, and is also used as an analytical reagent for the determination of amino acids. p-Benzoquinone is highly toxic, with a maximum allowable concentration of 0.1 ppm in the air, and is easily volatile and sublimated. It is highly irritating to the eyes, skin and mucous membranes, especially to the cornea. Long-term exposure can cause corneal disorders such as opacity of the lens of the eyeball and ulcers. Therefore, it is of great significance to develop a fast, simple and sensitive analytical method for the determination...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6402G01N21/6428G01N2021/6417G01N2021/6432
Inventor 王海波董高丽白宏宇李阳刘彦明
Owner XINYANG NORMAL UNIVERSITY
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