PIK3CA gene mutation detection primer probe and kit thereof

Abstract

The invention relates to a PIK3CA gene mutation detection primer probe and a kit thereof. The kit comprises a digital PCR premixed liquid for preparing a digital PCR reaction liquid, a drop stabilizer and a primer probe mixed liquid; the primer probe mixed liquid comprises upstream and downstream primers A and a mutation probe A for detecting the E542K site of the 542nd codon, upstream and downstream primers B and a mutation probe B for detecting the E545K site of the 545th codon, upstream and downstream primers C for detecting the 1047th codon, a probe C for detecting the H1047L site of the 1047th codon and a probe D for detecting the H1047R site of the 1047th codon. The PIK3CA gene mutation detection kit is high in sensitivity and specificity and small in quantity demand of samples, and can perform multiple PCR detection quickly, conveniently and accurately.

Description

technical field [0001] The invention relates to a primer, a probe and a detection product for detecting PIK3CA gene mutation, belonging to the field of biotechnology. Background technique [0002] Studies have found that PIK3CA amplification, deletion, and somatic mutations exist in a variety of human cancers (colon cancer, breast cancer, brain cancer, liver cancer, gastric cancer, and lung cancer, etc.). It is closely related to the occurrence and development of the tumor, and it is also closely related to the stage, grade and prognosis of the tumor. Therefore, detection of PIK3CA mutations in different stages of tumor patients is of great significance for judging the prognosis of patients [0003] Exploring the mechanism of PIK3CA drug resistance and predicting the prognosis of patients has become a feasible way. Now it is clear that tumor cells will release DNA into the circulation system, so that the specific nucleic acid sequences of early and advanced tumor patients c...

AI Technical Summary

Problems solved by technology

Due to the limitation of hardware equipment, it is difficult to realize simultaneous detection of multiple sites, and the dPCR multiplex

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