Comprehensive fluorescent and super-resolution microscopic imaging device

Abstract

The invention discloses a comprehensive fluorescent and super-resolution microscopic imaging device. According to the invention, firstly, a light beam is divided into four symmetrical beams of light paths through three polarization beam splitters. Secondly, after the propagation angle and the position of the beams are modulated by a 4f-vibration mirror system, the light beams are combined through two beam splitters. Thirdly, the light beams are transmitted to the back focal plane of a microscope objective in the convergence manner through a 4f system. Finally, the four light beams are shot onto a sample in the parallel manner through the microscope objective and the beam interference occurs. Through the position adjustment of a shutter, a vortex phase plate and a scanning vibration mirror in the four beams of light paths, the latticed interference structure type light illumination mode, the horizontal strip-shaped interference structure type light illumination mode and the longitudinal strip-shaped interference structure type light illumination mode can be formed. Meanwhile, the light period, the phase and the direction of each structure can be arbitrarily and quickly adjusted. The device can realize the functions of structure light illumination microscopic imaging, three-dimensional structure light illumination microscopic imaging, annular total internal reflection fluorescence microscopic imaging, fourier frequency shift iterative microscopic imaging and the like.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a comprehensive fluorescence super-resolution microscopic imaging device. Background technique [0002] Cells are the basic structural and functional units of all life activities. Exploring the life activities in cells can help people understand the life activities of human beings. Fluorescence super-resolution microscopy has the advantages of non-destructive, strong specificity, and deep penetration depth. Researches on resolution microscopic imaging technology and devices are also emerging one after another, and have important scientific value and practical significance. [0003] Currently, the mainstream super-resolution microscopy techniques include stimulated emission depletion microscopy (STED), stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), structural Structured-illumination microscopy (SIM), Fluorescence ...

AI Technical Summary

Problems solved by technology

For physical gratings and SLMs, the former requires a precision displacement servo system to translate and rotate them, while the latter is limited by the characteristics of SLM materials (mostly liquid crystals), and the time for them to obtain a frame of image is generally on the order of tens of ms. The highest shooting speed currently reported is about 100 frames, and the number of frames converted into super-resolution images is about 6 frames, which is still insufficient for the fast-changing biological movement process

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