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Application of apatinib and anti-PD-1 antibody combination to preparation of colon cancer medicines

A technology of apatinib and anti-pd-1, which is applied in the field of combined application of apatinib and anti-PD-1 antibody, which can solve the problem of less drugs and achieve the effect of increasing the area

Inactive Publication Date: 2017-07-21
顾艳宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although chemotherapy combined with targeted therapy has improved the overall survival rate of patients with advanced colorectal cancer, few drugs can be used for colorectal cancer patients who have progressed after multiple lines of chemotherapy, and chemotherapy-induced gastrointestinal reactions, bone marrow suppression , neurotoxicity and other adverse reactions often occur

Method used

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  • Application of apatinib and anti-PD-1 antibody combination to preparation of colon cancer medicines
  • Application of apatinib and anti-PD-1 antibody combination to preparation of colon cancer medicines
  • Application of apatinib and anti-PD-1 antibody combination to preparation of colon cancer medicines

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Embodiment

[0018] 1. Cell culture

[0019] Mouse colon cancer cell line CT26 was cultured in 1640 complete medium (containing 10% FBS) at 37°C, 5% CO 2 in a humidified incubator.

[0020] 2. Subcutaneous transplantation model of colon cancer in mice

[0021] The mouse colon cancer cell CT26 in the logarithmic growth phase was digested with trypsin, centrifuged at 250g for 5min, washed twice with pre-cooled PBS, and diluted to 2×10 7 / ml concentration. For 6-8 week-old Balb / c mice, the area around the right armpit and the hair on the right side of the body were shaved clean, and each mouse was injected subcutaneously with a volume of 100 μl. After the tumor grew out, the size of the tumor was measured with a vernier caliper every two days. Tumor volume calculation formula: V=0.5×L1×(L2) 2 , where L1 represents the tumor length and L2 represents the tumor width.

[0022] 3. Dosing regimen

[0023] When the tumor volume of most mice reaches 100-200mm 3 According to the tumor size, t...

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Abstract

The invention discloses application of apatinib and anti-PD-1 antibody combination to preparation of colon cancer medicines. The colon cancer is treated through combined medication of apatinib and an anti-PD-1 antibody; through experiments, the tumor inhibition rate of apatinib and anti-PD-1 antibody combination is 53.97 percent, the tumor inhibition rate is increased by 40.4 percent as compared with that of a single apatinib group (53.97 percent vs 13.57 percent, P is less than 0.05, Mann-Whitneytest), and the tumor inhibition rate is increased by 23.17 percent as compared with that of a single anti-PD-1 antibody group (53.97 percent vs 13.57 percent, P is less than 0.05, Mann-Whitneytest) (the tumor inhibition rate is equal to 1-expreiment group tumor volume / control group tumor volume). After the apatinib and the anti-PD-1 antibody are combined, the tumor necrosis area can be obviously increased as compared with that of single groups.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a combined application of apatinib and Anti-PD-1 antibody. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive tract. Its high prevalence rate has become a major problem in developed countries in Europe and the United States. In recent years, it has risen significantly worldwide. According to the latest forecast of Cancer Statistics, in 2017, new cases of colorectal cancer in American men will account for 9% of all tumors, ranking third among malignant tumors, and women will account for 8% of all tumors, second only to breast cancer and lung cancer . Also in my country, with the improvement of people's living standards and changes in dietary structure, the incidence of colorectal cancer continues to rise. In 2015, 376,300 people were diagnosed with colorectal cancer, accounting for about 8% of all diagnosed cancer patients. Among the common malig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K31/444A61P35/00
CPCA61K31/444A61K39/3955A61K2300/00
Inventor 顾艳宏徐强郭文洁李乐乐
Owner 顾艳宏
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