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Millettia specisoa antibacterial tissue culture medium and preparation method thereof

A technique of tissue culture medium and Niu Dali, which is applied in botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of unfavorable growth of tissue culture seedlings and bacterial growth, and achieve the purpose of inhibiting bacterial growth and improving the storage period. The effect of long, easy-to-operate preparation method

Inactive Publication Date: 2017-07-21
南宁市辉雄中药材农民专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the tissue culture medium contains a large amount of inorganic and organic substances, it is easy to cause bacterial growth, which is not conducive to the healthy growth of tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A bovine vigorous antibacterial tissue culture medium, comprising the following raw materials: 40g of agar, 15g of glucose, 0.3g of NAA, containing sodium borate, zinc chloride, ammonium chloride, potassium dihydrogen phosphate, magnesium chloride, manganese chloride, calcium chloride 3g of inorganic salt solution, 0.2-1.2g of sodium α-naphthalene acetate, 0.1g of silver carboxymethyl chitosan, 0.2g of riboflavin, 0.2g of zeatin, 10g of hydrolyzed casein, 55g of mashed potatoes, and 20g of triple-distilled water.

[0030] The preparation method of described bovine strong antibacterial tissue culture medium comprises the following steps:

[0031] S1: NNA is first dissolved with a small amount of 95% alcohol, and then added with deionized water to make a solution;

[0032] S2: Deionized water is used to configure glucose into a 10% solution;

[0033] S3: Add triple distilled water to the beaker, then add the NNA solution obtained in S1, inorganic salt solution, sodium α-...

Embodiment 2

[0038] A bovine vigorous antibacterial tissue culture medium, including the following raw materials: agar 110g, glucose 45g, 6-BA1.5g, containing sodium borate, zinc chloride, ammonium chloride, potassium dihydrogen phosphate, magnesium chloride, manganese chloride, chloride Calcium inorganic salt solution 15g, sodium α-naphthalene acetate 1.2g, carboxymethyl chitosan silver 0.6g, riboflavin 1.2g, zeatin 1.5g, hydrolyzed casein 30g, apple puree 125g, triple distilled water 60g.

[0039] The preparation method of described bovine strong antibacterial tissue culture medium comprises the following steps:

[0040] S1: Dissolve 6-BA with a small amount of 95% alcohol first, then add deionized water to make a solution;

[0041] S2: Deionized water is used to configure glucose into a 10% solution;

[0042] S3: Add triple distilled water to the beaker, then add the 6-BA solution obtained in S1, inorganic salt solution, sodium α-naphthalene acetate, silver carboxymethyl chitosan, ribo...

Embodiment 3

[0047]A bovine vigorous antibacterial tissue culture medium, comprising the following raw materials: agar 50g, glucose 18g, IBA 0.4g, containing sodium borate, zinc chloride, ammonium chloride, potassium dihydrogen phosphate, magnesium chloride, manganese chloride, calcium chloride 5 g of inorganic salt solution, 0.3 g of sodium α-naphthalene acetate, 0.2 g of silver carboxymethyl chitosan, 0.3 g of riboflavin, 0.3 g of zeatin, 12 g of hydrolyzed casein, 65 g of banana puree, and 25 g of triple distilled water.

[0048] The preparation method of described bovine strong antibacterial tissue culture medium comprises the following steps:

[0049] S1: IBA is first dissolved with a small amount of 95% alcohol, then add deionized water to make a solution;

[0050] S2: Deionized water is used to configure glucose into a 10% solution;

[0051] S3: Add triple distilled water to the beaker, then add the IBA solution obtained in S1, inorganic salt solution, sodium α-naphthalene acetate,...

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PUM

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Abstract

The invention discloses a millettia specisoa antibacterial tissue culture medium and a preparation method thereof, and belongs to the technical field of tissue culture mediums. The millettia specisoa antibacterial tissue culture medium comprises the following raw materials: agar, glucose, hormone, an inorganic salt solution, alpha-pimacol, carboxymethyl chitosan silver, riboflavin, zeatin, casein hydrolysate, organic matters and three-distilled water. The millettia specisoa antibacterial tissue culture medium which is prepared from the raw materials and the preparation method, which is disclosed by the invention, is prepared according to properties of nutrients essential for millettia specisoa tissue culture seedlings to grow, has a function of promoting growth and development of the millettia specisoa tissue culture seedlings, furthermore has an antibacterial function, and is capable of effectively inhibiting propagation of bacteria in culture mediums.

Description

technical field [0001] The invention belongs to the technical field of culture medium preparation, and in particular relates to a bovine vigorous antibacterial tissue culture medium. Background technique [0002] Niu Dali, also known as Zhujiao Li, Admiralty Root, Mountain Lotus Root, Upside Down Admiralty, and Dali Potato, is a plant of the genus Tachibana in the family Fabaceae, and its main components are protein, starch and alkaloids. Niu Dali uses its roots as medicine and can be excavated throughout the year. It is a traditional medicinal and edible plant. Niu Dali is flat in nature and sweet in taste, and has the effects of nourishing the kidneys and lungs, strengthening tendons and activating collaterals. It is often used to treat lumbar muscle strain, rheumatoid arthritis, tuberculosis, chronic bronchitis, chronic hepatitis and other diseases. In addition to being used as a medicinal decoction, Niu Dali is also often used as a soup for folks in Guangdong for dietar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 杨辉增
Owner 南宁市辉雄中药材农民专业合作社
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