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Rapid determination method of content of soluble proteins

A technology for rapid determination of protein content, which is applied in measurement devices, preparation and sampling of samples for testing, etc., can solve problems such as decline, and achieve the effects of accurate determination results and simple determination methods.

Inactive Publication Date: 2017-07-18
JIANGSU UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Usually, the dissolution of protein in the raw material will cause the pH of the solution to drop. Ultrasound-assisted extraction of protein can accelerate this change. At present, in the process of ultrasonic extraction, it is not yet possible to use the amount of alkali consumed to maintain pH stability to detect the content of soluble protein. see the report

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  • Rapid determination method of content of soluble proteins
  • Rapid determination method of content of soluble proteins
  • Rapid determination method of content of soluble proteins

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Preparation of protein suspension: Add distilled water according to 4% material-to-liquid ratio and mix evenly, that is, weigh 120 g of rice protein, add 3L of distilled water to make a suspension.

[0024] (2) Ultrasonic-assisted protein extraction: single-frequency concentrated ultrasonic equipment was used to assist in the extraction of rice protein, and the 28 kHz ultrasonic probe was completely immersed in the rice protein solution, and the ultrasonic parameters were set: power density 60 W / L, intermittent ratio 6:6 s , temperature 30°C, time 41 min. Use an automatic titrator to add 0.05 M NaOH dropwise to maintain the pH of the reaction solution. Before the start of ultrasound and 5 minutes after the ultrasound, record the amount of alkali consumed every 3 minutes. At the same time, 1 ml of the sample was taken at 5000 r / centrifuged under the condition of 1 min, and the protein content in the supernatant was determined by the Lowry method.

[0025] (3) Estab...

Embodiment 2

[0030] (1) Preparation of protein suspension: add distilled water according to 15% material-to-liquid ratio and mix evenly, that is, weigh 450 g of rice protein, add 3L of distilled water to make a suspension.

[0031] (2) Ultrasonic-assisted protein extraction: single-frequency focused ultrasonic equipment was used to assist in the extraction of rice protein. The 28 kHz ultrasonic probe was completely immersed in the rice protein solution, and the ultrasonic parameters were set: power density 60 W / L, intermittent ratio 6:6 s , temperature 30°C, time 41 min. Use an automatic titrator to add 0.06 M NaOH dropwise to keep the pH in the reaction solution constant. Before the start of sonication and 5 minutes after sonication, record the amount of alkali added every 3 minutes, and sample 1 ml at the same time, at 5000 r / centrifuged under the condition of 1 min, and the protein content in the supernatant was determined by the Lowry method.

[0032] (3) Establish a mathematical mod...

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Abstract

The invention discloses a rapid determination method of the content of soluble proteins and relates to the field of detection of the content of proteins. The invention provides a mathematical model for the content of the soluble proteins and an alkali adding amount in an ultrasonic-assisted protein extraction process and the model can be used for predicating the content of the soluble proteins at different time. The rapid determination method disclosed by the invention has the advantages that the established mathematical model has a very good linear relation and the alkali adding amount in a reaction process can be obtained online; a determination result of the proteins is accurate and reliable; compared with an offline determination method, the rapid determination method is simpler and more accurate and the aim of monitoring production in real time can be realized.

Description

technical field [0001] The invention relates to the field of protein content detection, in particular to a method for rapid determination of soluble protein content in an ultrasonic-assisted protein extraction process. Background technique [0002] As a non-thermophysical processing technology, ultrasonic is widely used in the process of auxiliary protein extraction due to its advantages of simple operation, easy control of reaction conditions, and the ability to realize industrial scale-up. With the same extraction rate as a measure, ultrasonic Compared with the traditional extraction process, the extraction time is greatly shortened. [0003] In the ultrasonic-assisted protein extraction process, the content of soluble protein in the extraction solution is crucial to the optimization of parameters and the control of the extraction end point. At present, the following methods are commonly used for the determination of soluble protein: Kjeldahl method, Lowry method, biuret ...

Claims

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Application Information

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IPC IPC(8): G01N31/22G01N1/40
CPCG01N31/22G01N1/4077G01N2001/4094
Inventor 杨雪马海乐李云亮黄姗芬王禹程赵丽霞陆峰魏康
Owner JIANGSU UNIV
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