Method for preparing saffron crocus extract for cigarette from immobilized beta-glucosidase

A technology of saffron extract and glucosidase, applied in biochemical equipment and methods, preparation of tobacco, immobilization on/in organic carriers, etc., can solve problems such as unreported saffron extract for tobacco, and achieve aroma Enrich, increase sweet aroma, increase yield

Inactive Publication Date: 2017-07-18
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is widely used in the fields of sewage treatment, pharmaceutical engineering, chemical synthesis, etc., but it has not been reported in the preparation of tobacco saffron extract

Method used

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  • Method for preparing saffron crocus extract for cigarette from immobilized beta-glucosidase
  • Method for preparing saffron crocus extract for cigarette from immobilized beta-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Get dried saffron flowers, add water of 5 times its weight, microwave extraction twice, each time for 1 hour, filter the extract after cooling, collect the filtrate to obtain the saffron extract;

[0019] (2) Dissolve 0.2g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 18mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 26mL The mass concentration was 2% (W / V) calcium chloride solution, embedded at 4°C for 1.5 h, and washed with buffer solution to obtain sodium alginate-immobilized enzyme with a loading capacity of 15 U / g.

[0020] (3) Get the immobilized β-glucosidase to process the saffron extract, the solid dosage of the immobilized β-glucosidase is 0.5g / L, the pH value of the enzyme treatment is 3~4, the enzyme treatment temperature is 35°C, and the enzyme treatment time is 30h, After the treatment is completed, settle at 3°C ​​for 36 hours;

[0021] (4) the extract after the enzyme treatment is ...

Embodiment 2

[0023] (1) Get dried saffron flowers, add water of 15 times its weight, microwave extraction twice, each time for 3 hours, filter the extract after cooling, collect the filtrate to obtain the saffron extract;

[0024] (2) Dissolve 0.1g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 15mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 20mL The mass concentration was 2% (W / V) calcium chloride solution, embedded at 4°C for 1 hour, and washed with buffer solution to obtain sodium alginate-immobilized enzyme with a loading capacity of 10 U / g.

[0025] (3) Get immobilized β-glucosidase to process saffron extract, immobilized β-glucosidase solid dosage 5g / L, the pH value of enzyme treatment 5~6, enzyme treatment temperature 30 ℃, enzyme treatment time 15h, process After completion, settle at 3°C ​​for 40 hours;

[0026] (4) the extract after the enzyme treatment is filtered with filter paper to remove large particles o...

Embodiment 3

[0028] (1) Get dried saffron flowers, add water of 10 times its weight, microwave extraction twice, each 2 hours, filter the extract after cooling, collect the filtrate to obtain the saffron extract;

[0029] (2) Dissolve 0.3g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 20mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 30mL The mass concentration was 2% (W / V) calcium chloride solution, embedded at 4°C for 3 hours, and washed with buffer solution to obtain sodium alginate immobilized enzyme with a loading capacity of 20 U / g.

[0030] (3) Get the immobilized β-glucosidase to process the saffron extract, the solid dosage of the immobilized β-glucosidase is 2g / L, the pH value of the enzyme treatment is 6~7, the enzyme treatment temperature is 40°C, and the enzyme treatment time is 5h. After completion, settle at 3°C ​​for 48 hours;

[0031] (4) the extract after the enzyme treatment is filtered with filter pap...

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Abstract

The invention provides a method for preparing a saffron crocus extract for cigarette from immobilized beta-glucosidase. The method comprises the following steps: (1) taking dry saffron crocus flowers, adding water and performing microwave extraction, thereby acquiring a saffron crocus extracting solution; (2) with sodium alga acid as a carrier, embedding, thereby acquiring sodium alga acid immobilized enzyme; (3) treating the saffron crocus extracting solution with immobilized beta-glucosidase; (4) filtering the extracting solution after enzyme treatment, concentrating the filtered saffron crocus extracting solution at reduced pressure and collecting a concentrated solution, thereby acquiring the saffron crocus extract for cigarette. According to the invention, the saffron crocus extract is treated with immobilized beta-glucosidase, the fragrance precusor substance in saffron crocus is hydrolyzed and the potential aroma constituent in saffron crocus is released, so that the extracting yield of the aromatic substance in saffron crocus is greatly increased, the production cost is lowered and the special cigarette perfume material with richer fragrance and higher quality can be acquired.

Description

technical field [0001] The invention relates to a method for preparing flavor and fragrance for tobacco, in particular to a method for preparing tobacco saffron extract by immobilizing β-glucosidase. Background technique [0002] In tobacco products, the aroma and taste of tobacco are very important. Adding spices is a key link in the cigarette process, and it is also one of the effective means to improve the product style and improve the quality of cigarette aroma. It can provide consumers with some aroma and Cigarette products with satisfying taste. At present, most of the tobacco flavors added to tobacco in my country are prepared by traditional methods, that is, mixed with natural extract monomers and synthetic monomers. The aroma is monotonous, the improvement of tobacco is insufficient, and the effect of improving the smoking quality of cigarettes is not obvious. [0003] Saffron (scientific name: Polianthes tuberosa), also known as evening primrose and moonflower, is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12N11/10C12N11/04A24B3/12
CPCA24B3/12C12N9/2445C12N11/04C12N11/10C12P7/26C12Y302/01021
Inventor 庞登红黄龙尹团章喻世涛王珊
Owner HUBEI CHINA TOBACCO IND
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