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Application of angiopoietin-like protein 4 in protecting barrier function of endothelia

An angiogenin and protein technology, applied in the biological field, can solve the problems of controversial status and unclear regulation mechanism of vascular permeability.

Inactive Publication Date: 2017-07-18
SHIJIAZHUANG YILING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the regulation mechanism of Angptl4 on vascular permeability is unclear, so its role in vascular permeability and tumor metastasis is controversial

Method used

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  • Application of angiopoietin-like protein 4 in protecting barrier function of endothelia
  • Application of angiopoietin-like protein 4 in protecting barrier function of endothelia
  • Application of angiopoietin-like protein 4 in protecting barrier function of endothelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Effect of high glucose and oxygen-glucose-serum deprivation / reoxygenation (OGSD / R) co-stimulation of angiopoietin-like protein 4 (Angptl4) on endothelial barrier function

[0059] The expression of Angptl4 in endothelial cells was knocked down by siRNA to confirm whether Angptl4 has the function of protecting endothelial cells.

[0060] 1. Human cardiac microvascular endothelial cells (HCMEC) were cultured in high glucose (18mM) (Table 1) medium, and the 5th-6th generation cells were randomly divided into the following groups, which were grown at T75 under the conditions of the following groups respectively. The cells were cultured in flasks, and then treated with oxygen-glucose-serum deprivation / reoxygenation (OGSD / R).

[0061] OGSD / R treatment includes: (1) use the corresponding medium to exchange medium to realize sugar-serum deprivation. Among them, the composition of cell culture medium for drug intervention and OGSD / R intervention is shown in Table 2. ...

Embodiment 2

[0145] Example 2. Angiopoietin-like protein 4 (Angptl4) alleviates diabetic myocardial ischemia-reperfusion injury by protecting endothelial barrier function

[0146] To ascertain the extent of myocardial infarction and the degree of reperfusion injury after acute myocardial infarction (AMI) reperfusion in non-diabetic lean mice and diabetic mice, the effect on myocardial microvascular endothelial cell apoptosis, and whether there is damage to myocardial microvascular endothelial barrier function , Can angiopoietin-like protein 4 reduce the size of myocardial infarction and reperfusion injury, apoptosis of myocardial microvascular endothelial cells and protect myocardial microvascular endothelial barrier function after AMI reperfusion in diabetic mice, the following experiments were carried out:

[0147] 1. 56 12-week-old male ZDF rats were randomly divided into the following groups:

[0148] 1. Diabetic mice sham operation group: LAD (left anterior descending artery (vessel))...

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Abstract

The invention discloses an application of angiopoietin-like protein 4 in protecting the barrier function of endothelia and discloses an application of the angiopoietin-like protein 4 in preparing a product for protecting and / or enhancing the shielding function of cells. The application proves that the angiopoietin-like protein 4 has the effect of protecting the shielding function of the endothelia, and can relieve myocardial-ischemia reperfusion injury of diabetes.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of angiopoietin-like protein 4 in protecting endothelial barrier function. Background technique [0002] Angiopoietin-like protein 4 (Angptl4) is a secreted glycoprotein, except Angptl5, Angptl1-8 are found in human and mouse. Human Angptl4 gene is highly conserved, with 77% amino acid sequence similarity to mouse. The human Angptl4 gene is located at 19p13.3, encoding a glycoprotein with a molecular weight of 45-65Kda. Similar to the Angptl family, Angptl4 consists of an N-terminal coiled domain and a C-terminal fibrinogen domain. Currently, Angptl4 is considered an orphan ligand, and its receptor has not been discovered yet. However, Angptl4 is activated by known activators of the PPAR family, glucocorticoid receptors, PKC, etc., and inhibited by angiotensin receptor blockers, inhibin, leptin, and insulin. Angptl4 is mainly expressed in adipose tissue, liver, skele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P3/10A61P9/10A61P9/00
CPCA61K38/1709
Inventor 杨跃进齐康崔贺贺李向东吴以岭魏聪常丽萍
Owner SHIJIAZHUANG YILING PHARMA
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