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Detection method of five types of compounds in Xuebijing injection

A detection method and compound technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of lack of comprehensive detection methods for the chemical components of Xuebijing injection.

Active Publication Date: 2017-07-14
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Application Information

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Problems solved by technology

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a detection method for the five types of compounds in Xuebijing injection, which is used to solve the lack of comprehensive detection of chemical compounds in Xuebijing injection in the prior art. The problem with the method of composition

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  • Detection method of five types of compounds in Xuebijing injection
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  • Detection method of five types of compounds in Xuebijing injection

Examples

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Effect test

Embodiment 1

[0101] 1. Sample pretreatment

[0102] Pipette a certain amount of Xuebijing Injection and dilute it 1-10000 times with 5-15% by volume methanol aqueous solution to prepare the test solution.

[0103] Pipette a certain amount of any one or more components of the first type of compound, the second type of compound, and the fifth type of compound except adenine and adenosine, and dilute with 5-15% methanol aqueous solution for 1 -10000 times, prepare standard solution A, the concentration range of each component in standard solution A is 12.3-9000nM.

[0104] Pipette a certain amount of any one or more components in the third class of compounds, dilute 1-10000 times with methanol aqueous solution with a volume percentage of 5-15%, and prepare standard solution B, the concentration range of each component in standard solution B 12.3-9000nM.

[0105] Pipette a certain amount of any one or more of the adenine and adenosine components in the fourth type of compound and the fifth t...

Embodiment 2

[0123] 1. Sample pretreatment

[0124] Pipette a certain amount of Xuebijing Injection and dilute it 10-1000 times with 10% by volume methanol aqueous solution to prepare the solution to be tested.

[0125] Pipette a certain amount of any one or more components of the first type of compound, the second type of compound, and the fifth type of compound except adenine and adenosine, and dilute 10-1000 with 10% by volume methanol aqueous solution. times, prepare a standard solution, the concentration range of each component in the standard solution is 12.3-9000nM.

[0126] 2. Determination

[0127] Adopt ultra-high performance liquid chromatography (UPLC) in the ultra-high performance liquid phase-mass spectrometry analysis system (UPLC / MS / MS), the first type compound, the second type compound, except adenine and Any one or more components in the fifth class of compounds other than adenosine were separated, and the triple quadrupole mass spectrometry (MS / MS) in the ultra-high pe...

Embodiment 3

[0143] 1. Sample pretreatment

[0144] Pipette a certain amount of Xuebijing Injection and dilute it 10-1000 times with 10% by volume methanol aqueous solution to prepare the solution to be tested.

[0145] Pipette a certain amount of any one or more components in the third class of compounds, dilute 10-1000 times with 10% methanol aqueous solution by volume, and prepare a standard solution. The concentration range of each component in the standard solution is 12.3-9000nM .

[0146] 2. Determination

[0147] Use ultra-high performance liquid chromatography (UPLC) in the ultra-high performance liquid phase-high resolution mass spectrometry analysis system (UPLC / Q-TOF MS) to use any one or more components in the third class of compounds in the test solution and standard solution Separation is carried out, and the high-resolution mass spectrometry (Q-TOF MS) in the ultra-high performance liquid phase-high resolution mass spectrometry analysis system (UPLC / Q-TOF MS) is used to det...

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Abstract

The invention provides a detection method of five types of compounds in Xuebijing injection. The detection method comprises the following steps: 1) preparing a solution to be detected; 2) preparing a standard solution; 3) simultaneously determining the content of any one or more components in a first type compound, a second type compound and a fifth type compound excepting adenine and adenosine by adopting an ultrahigh performance liquid chromatography-mass spectrometry system; 4) simultaneously determining the content of any one or more components of a third type compound by adopting an ultrahigh performance liquid chromatography-high resolution mass spectrometry system; 5) simultaneously determining the content of any one or more components in a fourth type compound and adenine and adenosine components in the fifth type compound by adopting the ultrahigh performance liquid chromatography-mass spectrometry system. The detection method of five types of the compounds in the Xuebijing injection, provided by the invention, can be used for accurately and comprehensively detecting chemical components in the Xuebijing injection in a high throughput manner; and the detection method has positive significance on improvement of quality control levels in an agent processing and production process and reasonable drug selection in clinical researches.

Description

technical field [0001] The invention belongs to the technical field of analysis of pharmaceutical ingredients, and relates to a detection method for five types of compounds in Xuebijing injection, in particular to a safflower flavonoid compound, red paeoniae monoterpene glycoside compound, The detection method of 47 compounds including salvia catechins, chuanxiong and angelica phthalides and other compounds. Background technique [0002] Sepsis is a critical illness with fatal organ dysfunction (SOFA score ≥ 2) caused by a dysregulated host response to infection. However, the current treatment drugs for sepsis are still very limited, and the success rate of new drug development is low. Xuebijing Injection is an anti-sepsis Chinese patent medicine developed and produced by Tianjin Hongri Pharmaceutical Co., Ltd. It was approved by the Chinese FDA in 2004. The traditional Chinese medicine preparation is prepared by extracting a compound composed of safflower, red peony root,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 李川程晨张纳婷李利牛巍
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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