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A detection method for five types of compounds in Xuebijing injection

A detection method and compound technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of methods for comprehensively detecting the chemical components of Xuebijing Injection

Active Publication Date: 2019-05-03
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a detection method for the five types of compounds in Xuebijing injection, which is used to solve the lack of comprehensive detection of chemical compounds in Xuebijing injection in the prior art. The problem with the method of composition

Method used

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  • A detection method for five types of compounds in Xuebijing injection
  • A detection method for five types of compounds in Xuebijing injection
  • A detection method for five types of compounds in Xuebijing injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] 1. Sample pretreatment

[0102] Pipette a certain amount of Xuebijing injection and dilute 1-10000 times with a methanol aqueous solution with a volume percentage of 5-15% to prepare the test solution.

[0103] Respectively pipet a certain amount of any one or more of the first compound, the second compound, and the fifth compound except adenine and adenosine, and dilute it with a methanol aqueous solution with a volume percentage of 5-15% 1 -10000 times, prepare standard solution A, the concentration range of each component in standard solution A is 12.3-9000nM.

[0104] Respectively pipet a certain amount of any one or more components of the third compound, and dilute 1-10000 times with a methanol aqueous solution of 5-15% by volume to prepare standard solution B. The concentration range of each component in standard solution B It is 12.3-9000nM.

[0105] Take a certain amount of any one or more of the adenine and adenosine components in the fourth compound and the fifth com...

Embodiment 2

[0123] 1. Sample pretreatment

[0124] Pipette a certain amount of Xuebijing injection and dilute 10-1000 times with a methanol aqueous solution with a volume percentage of 10% to prepare the test solution.

[0125] Take a certain amount of any one or more of the first type compound, the second type compound, and the fifth type compound except adenine and adenosine, and dilute 10-1000 with 10% methanol aqueous solution by volume. To prepare a standard solution, the concentration range of each component in the standard solution is 12.3-9000nM.

[0126] 2. Determination

[0127] Using ultra-high performance liquid chromatography (UPLC) in the ultra-high performance liquid-mass spectrometry analysis system (UPLC / MS / MS), the first type of compound, the second type of compound in the test solution and the standard solution, except for adenine and Any one or more components of the fifth group of compounds other than adenosine are separated, and the triple quadrupole mass spectrometry (MS / M...

Embodiment 3

[0143] 1. Sample pretreatment

[0144] Pipette a certain amount of Xuebijing injection and dilute 10-1000 times with a methanol aqueous solution with a volume percentage of 10% to prepare the test solution.

[0145] Respectively pipet a certain amount of any one or more of the third compounds, and dilute 10-1000 times with a 10% methanol aqueous solution to prepare a standard solution. The concentration range of each component in the standard solution is 12.3-9000nM .

[0146] 2. Determination

[0147] Adopt ultra-high performance liquid chromatography (UPLC) in the ultra-high performance liquid-high resolution mass spectrometry analysis system (UPLC / Q-TOF MS) to test any one or more components in the third type of compound in the test solution and standard solution Carry out separation, and use the high resolution mass spectrometry (Q-TOF MS) in the ultra-high performance liquid phase-high resolution mass spectrometry analysis system (UPLC / Q-TOF MS) to determine the third class of c...

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Abstract

The invention provides a detection method of five types of compounds in Xuebijing injection. The detection method comprises the following steps: 1) preparing a solution to be detected; 2) preparing a standard solution; 3) simultaneously determining the content of any one or more components in a first type compound, a second type compound and a fifth type compound excepting adenine and adenosine by adopting an ultrahigh performance liquid chromatography-mass spectrometry system; 4) simultaneously determining the content of any one or more components of a third type compound by adopting an ultrahigh performance liquid chromatography-high resolution mass spectrometry system; 5) simultaneously determining the content of any one or more components in a fourth type compound and adenine and adenosine components in the fifth type compound by adopting the ultrahigh performance liquid chromatography-mass spectrometry system. The detection method of five types of the compounds in the Xuebijing injection, provided by the invention, can be used for accurately and comprehensively detecting chemical components in the Xuebijing injection in a high throughput manner; and the detection method has positive significance on improvement of quality control levels in an agent processing and production process and reasonable drug selection in clinical researches.

Description

Technical field [0001] The invention belongs to the technical field of analysis of pharmaceutical ingredients, and relates to a detection method of five types of compounds in Xuebijing injection, and specifically relates to safflower flavonoids, red peony monoterpene glycoside compounds, and safflower flavonoids in Xuebijing injection. Methods for the detection of 47 compounds including Salvia miltiorrhiza catechol compounds, Ligusticum chuanxiong and Angelica phthalide compounds and other compounds. Background technique [0002] Sepsis is a critical illness of fatal organ dysfunction (SOFA score ≥ 2) caused by host dysregulated response caused by infection. However, the current treatment drugs for sepsis are still very limited, and the success rate of new drug development is low. Xuebijing injection is an anti-septic Chinese patent medicine developed and produced by Tianjin Hongri Pharmaceutical Co., Ltd. It was approved by the Chinese FDA in 2004 for marketing. The traditiona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 李川程晨张纳婷李利牛巍董凯
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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