Evaluating method of bacterioplankton community in Taihu Lake water body

Abstract

The invention provides an evaluating method of a bacterioplankton community in a Taihu Lake water body. The evaluating method comprises the steps of carrying out microbial genome DNA (Deoxyribose Nucleic Acid) extraction on water samples at 11 sampling points of a Taihu Lake area, quantitatively analyzing 16S rDNA (Ribosomal Deoxyribose Nucleic Acid) abundance in samples through carrying out common PCR (Polymerase Chain Reaction) amplification and real-time fluorescence quantification PCR on the extracted DNAs; purifying products subjected to rRNA (Ribosomal Ribonucleic Acid) gene PCR amplification of 16S, sequencing the purified products by adopting a second generation of high throughput sequencing technology, and after carrying out data processing and analysis on a sequencing result, analyzing the bacterioplankton community in the water body. The method genetically reveals a community structure of cyanobacterial bloom and the interaction with the environment, and predicates the phenomenon that an environment change may cause the change of the community structure; a second generation of high throughput sequencing Miseq platform is adopted for determining a microbial community structure in the Taihu Lake water body and bottom mud, so that the analysis of the community structure and the corresponding abundance of microorganisms in the Taihu Lake water body is more symmetrically assisted.

Description

technical field [0001] The invention relates to a method for evaluating the phytoplankton bacterial community in the water body of Taihu Lake, which uses group biology technology to evaluate the phytoplankton bacterial community in the water body of Taihu Lake, and belongs to the technical field of microbial analysis. Background technique [0002] The outbreak of cyanobacterial blooms is one of the important signs of lake eutrophication, which can cause a series of environmental and ecological problems. The accumulation of cyanobacteria blooms will lead to water surface coverage, making it difficult for aquatic plants to carry out effective photosynthesis, resulting in local hypoxia in water bodies, causing a large number of aquatic organisms such as fish to die, and may cause "lake flooding" in severe cases; , will release a large amount of microcystins, endangering the safety of the human body and the water ecosystem; the decline of cyanobacteria blooms will also cause ste...

AI Technical Summary

Problems solved by technology

However, due to limited conditions, most of the existing achievements are concentrated in the macroscopic field, while there are few researches on the microscopic field of cyanobacteria, such as organizational structure, growth form, and correlation with environmental factors.

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