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Extraction method of non-extractable polyphenols in blueberry

An extraction method and extraction technology are applied in the field of extraction of non-extractable polyphenols in blueberries, which can solve the problems of low NEPP activity, increased extraction difficulty, and many processing steps, and achieve low environmental pollution, mild method, and simple operation steps. Effect

Active Publication Date: 2020-10-09
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Blueberries contain high NEPP. Due to the limitation of the extraction process, non-extractable polyphenols have been ignored by researchers; and NEPP in blueberries is conjugated with dietary fiber protein, making it more difficult to extract
At present, most of the extraction methods of NEPP in blueberries are extracted by physical assistance methods. The extraction method has many processing steps, the extraction efficiency is low, and the activity of the extracted NEPP is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The specific operation steps are as follows:

[0051] 1. Prepare blueberry pomace: collect the residue after blueberry juice extraction, and dry at room temperature to obtain blueberry pomace;

[0052] 2. Prepare blueberry pomace powder: pulverize blueberry pomace and pass through an 80-mesh sieve to obtain blueberry pomace powder;

[0053] 3. Acid soaking: Put 10g of blueberry pomace powder into a 2L glass beaker, add 1000ml of 0.5mol / L hydrochloric acid solution for soaking, the soaking time is 12.5 hours, and the soaking temperature is 3°C to obtain an acidic soaking solution.

[0054] 4. Alkali soaking: Measure 50ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acidic soaking solution, and continue soaking for 3.5 hours at a temperature of 3°C to obtain an alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, there is 20×50÷1000=1mol of sodium hydroxide, after reacting with 0.5mol of hydrochloric ac...

Embodiment 2

[0062] The specific operation steps are as follows:

[0063] 1. Prepare blueberry pomace: collect the residue after blueberry juice extraction, and dry at room temperature to obtain blueberry pomace;

[0064] 2. Prepare blueberry pomace powder: pulverize blueberry pomace and pass through a 120-mesh sieve to obtain blueberry pomace powder;

[0065] 3. Acid soaking: put 10g of blueberry pomace powder into a 2L glass beaker, add 1200ml of 0.6mol / L hydrochloric acid solution for soaking, the soaking time is 11.5 hours, and the soaking temperature is 5°C to obtain an acidic soaking solution.

[0066] 4. Alkali soaking: Measure 71ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acidic soaking solution, and continue soaking for 2.5 hours at a temperature of 5°C to obtain an alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, there is 20×71÷1000=1.42mol of sodium hydroxide, after reacting with 1.2×0.6=0.72mol of hyd...

Embodiment 3

[0074] The specific operation steps are as follows:

[0075] 1. Prepare blueberry pomace: collect the residue after blueberry juice extraction, and dry at room temperature to obtain blueberry pomace;

[0076] 2. Prepare blueberry pomace powder: crush blueberry pomace and pass through a 100-mesh sieve to obtain blueberry pomace powder;

[0077] 3. Acid soaking: Put 10g of blueberry pomace powder into a 2L glass beaker, add 1100ml of 0.5mol / L hydrochloric acid solution for soaking, the soaking time is 12 hours, and the soaking temperature is 4°C to obtain an acidic soaking solution.

[0078] 4. Alkali soaking: Measure 54ml of saturated sodium hydroxide solution with a graduated cylinder, add it to the acidic soaking solution, and continue soaking for 3 hours at a temperature of 4°C to obtain an alkaline soaking solution. The concentration of saturated sodium hydroxide is 20mol / L, there is 20×54÷1000=1.08mol of sodium hydroxide, after reacting with 0.55mol of hydrochloric acid, ...

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Abstract

The invention relates to an extraction method of nonextractable polyphenol in blueberry, and belongs to the technical field of extraction of plant polyphenol. The extraction method comprises the followings steps: preparing blueberry residue, preparing blueberry residue powder, performing acid solution soaking, performing alkali solution soaking, performing enzymolysis, centrifugation, extraction and collecting extractive. The extraction method is simple in operation steps, the operation steps can be performed in one container, and the extractive is not required to be repeatedly collected; weak concentration acid and alkali are adopted to treat materials, ethyl acetate and ethyl alcohol mixed liquor can be utilized for extracting, the method is mild, the bioactivity of the nonextractable polyphenol can be furthest reserved, the extraction ratio is improved, and the environmental pollution is very small.

Description

technical field [0001] The invention belongs to the technical field of extracting plant polyphenols, and in particular relates to an extraction method of non-extractable polyphenols in blueberries. Background technique [0002] Blueberry, English name: Blueberry, meaning blue berry, belongs to Rhododendronaceae, Vaccinium plant, the fruit is dark blue, covered with white frost, nearly round, with delicate flesh, sweet and sour fruit. Blueberry fruit is rich in nutrients, which can prevent the aging of brain nerves, protect eyesight, strengthen the heart, fight cancer, soften blood vessels, and enhance human immunity. It has high nutritional content and is known as the "golden berry". One of the five recommended healthy fruits. In addition to conventional sugar, acid and vitamin C, blueberry fruit is rich in vitamin E, vitamin A, vitamin B, superoxide dismutase (SOD), arbutin, protein, anthocyanin, dietary fiber and rich potassium , iron, zinc, calcium and other mineral ele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105
CPCA23V2002/00A23V2200/3202A23V2250/2106A23V2250/2132
Inventor 张爱琳许程剑苗颖张登科郝程列
Owner TIANJIN AGRICULTURE COLLEGE
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