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Composition, kit and detection method for identifying authenticity of colla corii asini

A technology for authenticity identification and composition, which is applied in the field of compositions and kits for authenticating Ejiao, which can solve the problems of quantitative detection of donkey skin source components, discounts on accuracy and reliability, and supervision of Ejiao products in unfavorable markets.

Active Publication Date: 2017-06-20
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two reported methods are unable to carry out more accurate quantitative detection of donkey-hide gelatin-derived components, but to determine the authenticity of donkey-hide gelatin by detecting whether donkey-hide gelatin contains donkey components and excluding other animal-derived components; but the donkey-hide gelatin Adulteration may be varied, so there will be a variety of animal-derived ingredients that need to be excluded. There are important flaws in using the exclusion method to identify the authenticity of donkey-hide gelatin, and its accuracy and reliability will be greatly reduced. This situation is very unfavorable to the market for donkey-hide gelatin. product regulation

Method used

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  • Composition, kit and detection method for identifying authenticity of colla corii asini
  • Composition, kit and detection method for identifying authenticity of colla corii asini

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The detection of embodiment 1 pure product glue

[0033] 1 Materials and Reagents

[0034] Materials: All kinds of pure glue, including donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, sheepskin glue, fish skin glue (the reference substance detection matrix), donkey skin, horse hide, cowhide, pigskin, sheepskin and Obtained by decocting fish skin.

[0035] Polypeptide I (shown in SEQ ID NO.1, purity 99.2%), polypeptide II (shown in SEQ ID NO.2, purity 99.5%), polypeptide III (shown in SEQ ID NO.3, purity 99.2%), delivered to Biological company synthesis.

[0036] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure)

[0037] 2 detection method

[0038] 1) Preparation of matrix solution: Weigh 0.50 g of reference substance detection matrix into a 250ml measuring bottle, add a small amount of 1% (w / w) NH 4 HCO 3 solution (pH8.0), ultrasonically dissolve the sample completely, and use 1% (w / w) NH 4 HCO 3 Solution (pH8.0) diluted to...

Embodiment 2

[0053] The detection of embodiment 2 mixed rubber samples

[0054] 1 Materials and Reagents

[0055] Material: the mixed gum sample is made by adding donkey-hide gelatin of different qualities to the pure product gum sample in embodiment 1, comprises the yellow gelatin containing 15% (w / w) donkey-hide gelatin, the yellow gelatin containing 30% (w / w) donkey-hide gelatin , yellow gelatin containing 60% (w / w) donkey-hide gelatin, horse hide glue containing 30% (w / w) donkey-hide gelatin, and pigskin glue containing 30% (w / w) donkey-hide gelatin, respectively as samples 1-5.

[0056] Polypeptide I (shown in SEQ ID NO.1, purity 99.2%), polypeptide II (shown in SEQ ID NO.2, purity 99.5%), polypeptide III (shown in SEQ ID NO.3, purity 99.2%), delivered to Biological company synthesis.

[0057] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequential pure).

[0058] 2 detection method

[0059] 1) Preparation of matrix solution: Weigh 0.50 g of reference substance detec...

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Abstract

The invention discloses a composition, a kit and a detection method for identifying the authenticity of colla corii asini. The composition is prepared from polypeptide I, polypeptide II, polypeptide III and a reference substance detection substrate, wherein the amino acid sequence of the polypeptide I is as shown in SEQ ID No. 1, the amino acid sequence of the polypeptide II is as shown in SEQ ID No. 2, the amino acid sequence of the polypeptide III is as shown in SEQ ID No. 3, and the reference substance detection substrate is fish-skin gelatin. The composition can be used for detecting the content of a donkey skin-derived component in the colla corii asini, thus judging the authenticity of the colla corii asini. Specifically, a detection value of the polypeptide II is subtracted from a detection value of the polypeptide I, and a detection error is corrected by a detection value of the polypeptide III, so that the content of the donkey skin-derived component in the colla corii asini can be detected, and the authenticity of the colla corii asini can be identified according to the content of the donkey skin-derived component. The method has the advantages of accurate content detection and simple operation, thus having wide application prospect in the aspect of authenticity identification of the colla corii asini.

Description

technical field [0001] The invention relates to a composition and a kit for identifying the authenticity of donkey-hide gelatin, and also includes a method for authenticating donkey-hide gelatin by using the composition or the kit. The invention belongs to the technical field of medicine and food detection. Background technique [0002] Ejiao, as a traditional precious Chinese medicine, has been eaten for thousands of years. It has the functions of nourishing blood, nourishing yin, moistening dryness and stopping bleeding. The Chinese Pharmacopoeia stipulates that donkey-hide gelatin is a solid gelatin obtained by decocting and concentrating the dried skin or fresh skin of Equus asinus L., an equine animal donkey. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the efficacy of the product. From the perspective of raw material taxonomy, donkeys belong to the genus Equus of the order Perissodactyla, including African wild asses, Tibetan wild asses, Central ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 周祥山郝向慧郭尚伟段小波徐云鹏王静王玉娇石永坚张春辉
Owner SHAN DONG DONG E E JIAO
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