Real-time quantitative PCR detection method for ulva linza, hard ulva and scytosiphon lomentaria spores/gametes in holothurian culture water

Abstract

The invention relates to the field of prevention, control and early warning for moss outbreak of holothurian culture water and specifically relates to a real-time quantitative PCR detection method for ulva linza, hard ulva and scytosiphon lomentaria spores / gametes in holothurian culture water. Ulva linza, hard ulva and scytosiphon lomentaria are the important species of the moss outbreak of holothurian culture pond, but the spores / gametes individuals thereof are smaller and are difficult to be detected according to a traditional method. Specific primers are designed for three large-scale algae ITS areas, the fluorogenic quantitative PCR condition is optimized and lastly the quantitative PCR method is utilized to monitor the dynamic conditions of the three large-scale alga spores / gametes in the culture water in real time. A result proves that the dynamic change of the ulva linza, hard ulva and scytosiphon lomentaria spores / gametes in holothurian culture water is more effectively matched with the moss outbreak rule. The method provided by the invention can be used for quickly, efficiently and high-sensitively monitoring the dynamic change of the three large-scale algae in the holothurian culture water and supplying early warning for the possible moss outbreak.

Description

technical field [0001] The invention relates to the field of prevention and control and early warning of moss outbreaks in sea cucumber culture waters, in particular to a dynamic monitoring method for spores / gametes of Ulva ulva, Ulva hardina and Hemerocybe spores in sea cucumber culture water. Background technique [0002] "Moss" is a common name for the bloom-prone macroalgal organisms in aquaculture water. In recent years, in the process of sea cucumber culture in ponds, there has been an increasing trend of large moss algal blooms. Large algal blooms compete with beneficial microalgae (diatoms, etc.) for limited resources such as light and nutrients, resulting in slow or even lack of growth of bait organisms, limiting the growth of sea cucumbers; algal blooms may be accompanied by the secretion of toxins, causing physiological stress on sea cucumbers and chemical poisoning; the filamentous structure of moss will trap sea cucumbers in it, affecting the movement and feedi...

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Problems solved by technology

[0004] However, due to the small size (<10 μm) of germ cells such as sporophytes and gametophytes, it is difficult to distinguish them from other single-celled protists (such as autotrophic and mixed-trophic flagellates) under a light microscope, and only by traditional morphological observation It is difficult to determine its quantity or biomass, so the detection and dynamic monitoring of the sporophytes and gametophytes of macromoss algae in environmental samples cannot be realized

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