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High Dynamic Range Image Imaging Method Based on 3D Digital Microscopic Imaging System

A high dynamic range, digital microscopy technology, used in microscopy, image analysis, image enhancement, etc., can solve the problem of inability to capture high-definition dynamic scenes, overcome the inability to see reflective and non-reflective areas at the same time, and reduce computational complexity Effect

Active Publication Date: 2020-04-17
THE UNIV OF NOTTINGHAM NINGBO CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high dynamic range image imaging method can solve the defect that existing image imaging methods cannot capture high dynamic scenes, and can simultaneously and accurately generate the three-dimensional shape of the object to be observed, thereby providing observers with a full range of 3D stereoscopic visual enjoyment

Method used

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  • High Dynamic Range Image Imaging Method Based on 3D Digital Microscopic Imaging System
  • High Dynamic Range Image Imaging Method Based on 3D Digital Microscopic Imaging System
  • High Dynamic Range Image Imaging Method Based on 3D Digital Microscopic Imaging System

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Experimental program
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Effect test

Embodiment 1

[0075] like figure 2 As shown, the 3D digital microscopic imaging system used in the first embodiment includes a traditional optical microscope, an automatic stage capable of moving in any direction of the X-axis, Y-axis and Z-axis, a CMOS camera and a computer. Wherein, the object to be observed in the first embodiment is a metal screw, and the metal screw is placed on the automatic stage. see figure 1 As shown in , the high dynamic range image imaging method based on the 3D digital microscopic imaging system in the first embodiment includes the following steps:

[0076] Step 1. For the object to be observed on the microscope stage, that is, the metal screw, by adjusting the height of the stage, the CMOS camera is focused on each layer of the object to be observed, that is, each layer of the metal screw, And use the camera to obtain high dynamic multi-focus images of each layer from the bottom of the metal screw to the top of the metal screw to obtain the original high dyn...

Embodiment 2

[0116] In the second embodiment, a plastic bank card is used as the object to be observed, and the bank card has a lowercase English letter "d". Wherein, the steps for generating the three-dimensional image of the bank card are the same as the steps for generating the three-dimensional image of the metal screw in Embodiment 1, and will not be repeated here.

[0117] In the second embodiment, in order to verify the accuracy and robustness of the high dynamic range image imaging method of the present invention, the second embodiment provides the corresponding high dynamic range image generated by the bank card. For details, see Figure 7 a~ Figure 7 shown in f. Figure 8 For the bank card in the second embodiment, the method of generating a 3D stereoscopic shape using a high dynamic range image and the square root error comparison diagram without using a high dynamic range image.

[0118] Depend on Figure 8 It can be seen that for the same focusing factor, the square root e...

Embodiment 3

[0120] In the third embodiment, a metal chip is used as the object to be observed. Wherein, the steps for generating the three-dimensional image of the metal chip are the same as the steps for generating the three-dimensional image of the metal screw in Embodiment 1, and will not be repeated here.

[0121] Figure 10 The square root error comparison diagram of the 3D stereoscopic image generated by the method of generating 3D stereoscopic shape for high dynamic range image and the method of generating 3D stereoscopic shape of original automatic exposure image.

[0122] Depend on Figure 10 It can be seen that for the same focusing factor, the square root error value of the focusing factor corresponding to the 3D stereoscopic shape generated by using the high dynamic range image is smaller than the square root error value of the focusing factor corresponding to the 3D stereoscopic shape not generated by using the high dynamic range image. It can be seen that the 3D stereoscop...

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Abstract

The invention relates to a highly dynamic range imaging method based on a 3D digital microscopic imaging system. Image registration and movements on the super pixel level are conducted through the generation of high dynamic range images of a to-be-observed object and the acquisition of original high dynamic multi-focus sequence images of the to-be-observed sample by using the phase matching method and Fourier transformation. The foreground and background division method is used for singling out a target object. Four binary tree decomposition is conducted to the singled-out image. The clear image blocks are marked in the image sequence, and the height information corresponding to each image is recorded. Finally, the marked clear image blocks are fused into the three dimensional stereo shape of the to-be-observed object, and the median filtering is applied for filtering the generated three dimensional stereo shape to eliminate the sawtooth effect of the three dimensional stereo shape caused by insufficient sampling frequencies and make the generated three dimensional stereo shape of the to-be-observed object smoother.

Description

technical field [0001] The invention relates to the technical field of high-definition and high-precision microscopic imaging detection, in particular to a high dynamic range image imaging method based on a 3D digital microscopic imaging system. Background technique [0002] Multi-focal length 3D technology (Shape from Focus, referred to as SFF) is currently a commonly used 3D technology in the field of digital microscopic image processing. Since the multi-focal length 3D technology can obtain the three-dimensional shape of the observed sample only by using a traditional monocular microscope, it has attracted widespread attention from experts and scholars. Different from stereo vision technology that uses binocular lens to obtain depth information, multi-focal length 3D technology only detects clear areas in the image by moving and observing the distance from the object to the lens, so as to restore and reconstruct the depth information of the object. [0003] However, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/30G06T7/10G06T7/194G02B21/36
CPCG02B21/365G06T2207/10061
Inventor 郑驰邱国平
Owner THE UNIV OF NOTTINGHAM NINGBO CHINA
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