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A kind of coating method of double biomimetic dopamine phosphorylcholine substance

A technology of dopamine phosphorylcholine and phosphorylcholine, which is applied in the coating field of double biomimetic dopamine phosphorylcholine substances, can solve problems such as difficulties in preparation, and achieve broad application prospects, mild conditions, and simple preparation methods

Inactive Publication Date: 2020-11-13
XIAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Gong et al. synthesized bis-biomimetic polymers with high and controllable dopamine content using the active ester monomer approach, it is extremely difficult to prepare the active ester monomer in an anhydrous environment.

Method used

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  • A kind of coating method of double biomimetic dopamine phosphorylcholine substance
  • A kind of coating method of double biomimetic dopamine phosphorylcholine substance
  • A kind of coating method of double biomimetic dopamine phosphorylcholine substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 10 mmol 2-methacryloxyethyl phosphorylcholine and 11 mmol dopamine hydrochloride, dissolve them in methanol and mix them uniformly. in N 2 Protected, under the condition of stirring at 40°C, add the mixed solution and a small amount of triethylamine into the three-neck flask, and react for 20 hours. After the reaction, a double biomimetic dopamine phosphorylcholine substance solution is obtained, which is denoted as DA / MPC.

[0036]Take the double biomimetic dopamine phosphorylcholine substance solution and dilute it to 4.0 mg / mL, pipette 30 μL drop-coated on the surface of chitosan (CS) and polycarbonate (PC) substrate (18mm×18mm), After drying, the sample pieces were dried at 110°C for 2 hours and recorded as CS / DA / MPC and PC / DA / MPC respectively. Soak in Tris-HCl solution with pH = 8.5 and heat at 90°C for 8 hours, then wash with a large amount of methanol and distilled water in turn to obtain double bionic modified materials, which are respectively denoted as ...

Embodiment 2

[0040] Weigh 10mmol 2-methacryloyloxyethylphosphorylcholine and 12mmol dopamine hydrochloride, dissolve in ethanol and mix well. in N 2 protection, under the condition of stirring at 30°C, add the mixed solution and a small amount of triethylamine into the three-neck flask, and react for 24 hours. After the reaction, a solution of bis-bionic dopamine phosphorylcholine substance is obtained.

[0041] Take the double biomimetic dopamine phosphorylcholine substance solution and dilute it to 2.0mg / mL, pipette 100μL with a pipette gun and drop-coat it on the surface of chitosan (CS) and polycarbonate (PC) substrates (18mm×18mm), After drying, the sample sheet was dried at 100°C for 4 hours, soaked in a Tris-HCl solution with pH=8.5 and heated at 70°C for 12 hours, and washed with a large amount of methanol and distilled water in turn to obtain the double biomimetic modified material .

Embodiment 3

[0043] Weigh 10 mmol of 2-methacryloxyethyl phosphorylcholine and 11.05 mmol of dopamine hydrochloride, dissolve them in isopropanol and mix well. in N 2 Protected, under the condition of stirring at 35°C, add the mixed solution and a small amount of triethylamine into the three-neck flask, and react for 22 hours. After the reaction, a bis-bionic dopamine phosphorylcholine substance solution is obtained.

[0044] Take the double biomimetic dopamine phosphorylcholine substance solution and dilute it to 6.0 mg / mL, pipette 80 μL drop-coated on the surface of chitosan (CS) and polycarbonate (PC) substrate (18mm×18mm), After drying, the sample sheet was dried at 120°C for 1 hour, soaked in a Tris-HCl solution with pH=8.5 and heated at 80°C for 10 hours, and washed with a large amount of methanol and distilled water in sequence to obtain the double biomimetic modified material .

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Abstract

The invention discloses a synthesis and coating method of a double bionic dopamine and phosphorylcholine substance. The synthesis and coating method comprises the following steps of enabling a vinyl monomer containing a phosphorylcholine group and dopamine hydrochloride to generate Michael addition reaction in a polarity solvent at certain temperature, so as to obtain the double bionic dopamine and phosphorylcholine substance; thinning a reaction solution, coating the surface of the to-be-modified material, airing, heating at high temperature, treating by a Tris-HCl solution, adhering to the surface of a substrate, and building a bionic cell outer membrane structure. A preparation method of a coating with the bionic cell outer membrane structure has the advantages that the preparation method is simple, and the conditions are mild; a novel path is provided for obtaining a stable phosphorylcholine bionic coating, and the important academic meaning is realized for modifying the biological compatibility of the biological material. A modifying material with the bionic cell outer membrane structure has broad application prospect in the fields of in-vivo implant materials, tissue engineering, medicine slow release, biological sensors and the like.

Description

technical field [0001] The invention relates to the technical fields of material surface science and biomedical polymer materials, in particular to a method for coating double biomimetic dopamine phosphorylcholine substances. Background technique [0002] When the material is used in a living body, it is easy to non-specifically adsorb proteins, activate complement molecules and the immune system, thereby causing blood coagulation, immune and inflammatory reactions, resulting in a significant decrease in its performance or even failure. This is due to the poor biocompatibility of materials, therefore, biocompatibility research has become the primary issue in the field of biomaterial research. The surface of a material is the medium through which the material contacts the organism. Surface charge, hydrophilicity / hydrophobicity, chemical composition, and morphology are important factors that affect the interface interaction between the material and the organism, and determine ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/06C07F9/09C08L5/08C08L69/00
CPCC07F9/091C08J7/06C08J2305/08C08J2369/00
Inventor 宫铭
Owner XIAN UNIV OF SCI & TECH
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