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Method for extracting and separating mangiferin from mango leaves

A technology for mango leaves and mangiferin is applied in the field of extraction and separation of mangiferin, which can solve the problems of complex process and high extraction cost, and achieve the effects of simple operation and cost reduction.

Active Publication Date: 2017-06-13
PANZHIHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the existing methods for extracting and separating mangiferin from mango leaves generally have relatively complicated processes. Generally, products with a purity of more than 80% must be obtained through chromatographic separation, and the extraction cost is also relatively high.

Method used

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  • Method for extracting and separating mangiferin from mango leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A. Raw material preparation: take fresh mango leaves as raw materials, dry them naturally or at 50°C, and crush them through a 20-mesh sieve.

[0029] B. Ester extraction and impurity removal: Weigh 1000g of mango leaf powder, add 3L of ethyl acetate, seal and soak for 5h, extract by shaking at room temperature for 15min, and filter; repeat extraction 4 times, the green color of the extract basically disappears.

[0030] C. Methanol extraction: Take the residue extracted in step B, add 2.5L of methanol, seal and soak at 30°C for 5h, stir and extract at 55°C for 0.5h, filter while hot, repeat extraction 5 times, combine the extracts, and precipitate at room temperature 9h, finely filter with a 200-mesh filter.

[0031] D. Concentration and washing: Concentrate the filtrate of step C by vacuum rotary evaporation at 50°C to obtain about 50g of concentrated extract, add 150mL of methanol, stir and wash at 55°C for 10min, let cool, filter with a 200-mesh filter, and wash rep...

Embodiment 2

[0033] A. Raw material preparation: take fresh mango leaves as raw materials, dry them in the sun, and crush them through a 30-mesh sieve.

[0034] B. Ester extraction and impurity removal: Weigh 1000g of mango leaf powder, add 4L of ethyl acetate, seal and soak for 4h, shake at room temperature for 10min, filter; repeat extraction 3 times, the green color of the extract basically disappears.

[0035] C. Methanol extraction: Take the residue extracted in step B, add 4L of methanol, seal and soak at 40°C for 4h, stir and extract at 60°C for 0.5h, filter while hot, repeat the extraction 4 times, combine the extracts, and precipitate at room temperature for 10h , Finely filter with a 200-mesh filter.

[0036] D. Concentration and washing: Concentrate the filtrate of step C into a concentrated extract by vacuum rotary evaporation at 65°C, add 200mL of methanol, stir and wash at 50°C for 20min, let it cool, filter with a 200-mesh filter, and wash 5 times repeatedly. Yellow-white p...

Embodiment 3

[0039] A. Raw material preparation: take fresh mango leaves as raw materials, dry them at 65°C, and crush them through a 40-mesh sieve.

[0040] B. Ester extraction and impurity removal: Weigh 1000g of mango leaf powder, add 2L of ethyl acetate, seal and soak for 6h, shake at room temperature for 20min, filter; repeat extraction 5 times, the green color of the extract basically disappears.

[0041] C. Methanol extraction: take the residue extracted in step B, add 2L of methanol, seal and soak at 30°C for 6h, stir and extract at 60°C for 1h, filter while hot, repeat the extraction 6 times, combine the extracts, and precipitate at room temperature for 12h, Finely filter with a 200-mesh filter.

[0042]D. Concentration and washing: Concentrate the filtrate of step C into a concentrated extract by vacuum rotary evaporation at 65°C, add 130mL of methanol, stir and wash at 50°C for 20min, let it cool, filter with a 200-mesh filter, and wash 5 times repeatedly. Yellow-white particle...

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Abstract

The invention belongs to the field of plant extraction, and specifically relates to a method for extracting and separating mangiferin from mango leaves. The technical problem to be solved by the invention is to provide a brand-new method for extracting and separating the mangiferin from the mango leaves. The method is simple and easy to operate, and the mangiferin with relatively high purity can be obtained. The extraction method disclosed by the invention comprises the following steps: A, raw material preparation; B, ester extraction and impurity removal; C, methyl alcohol extraction; D, concentration washing, so that a mangiferin crude product with the purity of 60% to 70% can be obtained; the extraction method further comprises the step E of crystallization purification, so that a mangiferin pure product with the purity of 90% to 98% can be obtained. According to the extraction method disclosed by the invention, the relatively high mangiferin can be obtained just by using several simple extraction and separation means, so that the operation is simple and convenient, and the cost is greatly reduced. According to the extraction method disclosed by the invention, the mangiferin crude product with the purity of 60% to 70% and the mangiferin pure product with the purity of 90% to 98% can be obtained respectively, the yield of the mangiferin crude product is greater than or equal to 83%, and the yield of the pure product is greater than or equal to 65%.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a method for extracting and separating mangiferin from mango leaves. Background technique [0002] Mangiferin (mangiferin) is a carbon ketone glycoside of tetrahydroxypyrrole, molecular formula C 19 h 18 o 11 , the structural formula is shown in formula 1, and it is widely found in many plants such as mango, anemarrhena, capillary, and waterweed. In the Chinese Pharmacopoeia and the quality standards of various Chinese medicinal materials and Chinese patent medicines, the content of mangiferin is widely used as a quality control indicator. Modern medical research has proved that mangiferin has various pharmacological effects such as anti-oxidation, anti-inflammation, anti-radiation, prevention and treatment of skin aging, lowering blood sugar, lowering uric acid, anti-tumor, and immune regulation, and has a very high development and utilization prospect. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 韦会平韩洪波尚远宏刘文权田金凤王丹何妍羲
Owner PANZHIHUA UNIV
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