A kind of green fruit extract and its preparation method and application
A technology of extract and cypress fruit, applied in the directions of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve problems such as water and electrolyte disorders, nutritional disorders, and easy recurrence, and achieve the effects of improving abdominal pain, reducing expression, and improving SOD activity.
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Embodiment 1
[0024] Preparation of Green Fruit Extract
[0025] (1) After crushing 500g green fruit, pass through a 40-mesh sieve, take the under-sieve, and use supercritical fluid extraction method to extract it with supercritical CO 2 As a supercritical extractant, the green fruit with a particle size of -40 mesh was extracted. The extraction was carried out at 40°C and 300 bar at a speed of 0.62g / min for 6 hours, and the extract was collected every 20 minutes;
[0026] (2) Using a supercritical fluid extraction device, with acetone as the extraction agent, the green fruit after the treatment in step (1) is extracted again to obtain the extract A. Extract at a rate of g / min for 6 hours, and collect the extract every 20 minutes;
[0027] (3) Using a supercritical fluid extraction device, water is used as the extraction agent to extract the green fruit after the treatment in step (2) again to obtain the extract B. Extract at a rate of g / min for 6 hours, and collect the extract every 20 m...
Embodiment 2
[0030] Preparation of Green Fruit Extract
[0031] (1) After crushing 400g green fruit, pass it through a 40-mesh sieve, take the under-sieve, and use supercritical fluid extraction method to extract it with supercritical CO 2 As a supercritical extractant, the green fruit with a particle size of -40 mesh was extracted. The extraction was carried out at 45°C and 350 bar at a speed of 0.58g / min for 6 hours, and the extract was collected every 20 minutes;
[0032] (2) Using a supercritical fluid extraction device, with acetone as the extraction agent, the green fruit after the treatment in step (1) is extracted again to obtain the extract A. Extract at a rate of g / min for 6 hours, and collect the extract every 20 minutes;
[0033] (3) Using a supercritical fluid extraction device, water is used as the extraction agent to extract the green fruit after the treatment in step (2) again to obtain the extract B. Extract at a rate of g / min for 6 hours, and collect the extract every 2...
Embodiment 3
[0036] Preparation of Green Fruit Extract
[0037] (1) After crushing 500g green fruit, pass through a 40-mesh sieve, take the under-sieve, and use supercritical fluid extraction method to extract it with supercritical CO 2 As a supercritical extractant, the green fruit with a particle size of -40 mesh was extracted. The extraction was carried out at a speed of 1.0 g / min for 6 hours at 35°C and 450 bar, and the extract was collected every 20 minutes;
[0038] (2) Using a supercritical fluid extraction device, with acetone as the extraction agent, the green fruit after the treatment in step (1) is extracted again to obtain the extract A. Extract at a rate of g / min for 6 hours, and collect the extract every 20 minutes;
[0039] (3) Using a supercritical fluid extraction device, water is used as the extraction agent to extract the green fruit after the treatment in step (2) again to obtain the extract B. Extract at a rate of g / min for 6 hours, and collect the extract every 20 m...
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