Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of ACTA1 as diagnosis and treatment marker of tongue squamous cell carcinoma

A tongue squamous cell carcinoma, substance technology, applied to ACTA1 gene in the diagnosis and treatment of tongue squamous cell carcinoma, can solve the problem that the molecular mechanism is not clear.

Active Publication Date: 2017-06-09
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although researchers have successively discovered that dozens of oncogenes or tumor suppressor genes are involved in the occurrence and development of tongue cancer, so far, the molecular mechanism of tongue cancer has not been clarified, and there may be other tumor-related genes involved. The pathogenesis of tongue cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of ACTA1 as diagnosis and treatment marker of tongue squamous cell carcinoma
  • Use of ACTA1 as diagnosis and treatment marker of tongue squamous cell carcinoma
  • Use of ACTA1 as diagnosis and treatment marker of tongue squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Differential expression of ACTA1 gene

[0066] 1. Experimental materials

[0067] 5 tissue samples of tongue squamous cell carcinoma were obtained from oral and maxillofacial surgery patients, including 2 cases of well-differentiated squamous cell carcinoma, 2 cases of moderately differentiated squamous cell carcinoma, and 1 case of poorly differentiated squamous cell carcinoma; including 2 males and 3 females. At the same time, each case of cancerous tissue was selected as the normal tissue at >5cm around the cancerous tumor as its own control. All patients had not undergone chemotherapy, radiotherapy, biological therapy and other treatments for tumors before seeing the doctor. A part of the tissue was immediately stored in liquid nitrogen after collection.

[0068] 2. RNA extraction and cDNA synthesis

[0069] Total RNA was extracted with Trizol RNA reagent (Invitrogen Company), and total RNA was identified by UV spectrophotometer (ND-1000, NanoDrop Company...

Embodiment 2

[0078] Example 2 Verification of Differentially Expressed Genes in Large Samples

[0079] 1. Research object

[0080] According to the method of Example 1, 45 cases of tongue squamous cell carcinoma tissue samples and 50 cases of normal tissue samples were collected.

[0081] 2. RNA extraction and cDNA synthesis

[0082] RNA extraction and cDNA synthesis were performed according to the method in Example 1.

[0083] 3. RT-PCR

[0084] The primers were designed by the primer design software Primer 5.0, and synthesized by Dalian Bao Biological Company and Shanghai Yingjun Company. The primer sequences used for ACTA1 gene and internal reference gene are as follows:

[0085] ACTA1 gene primer sequence

[0086] 5'-ATTCACGAGACCACCTAC-3' (SEQ ID NO.1),

[0087] 5'-ATGACGTTGTTGGCATAC-3' (SEQ ID NO.2);

[0088] GAPDH gene primer sequence

[0089] 5'-AAGGTCGGAGTCAACGGATTTG-3' (SEQ ID NO.3),

[0090] 5'-CCATGGGTGGAATCATATTGGAA-3' (SEQ ID NO. 4).

[0091] 1 μl of cDNA product was...

Embodiment 3

[0100] Example 3 Determination of the expression of ACTA1 gene on the proliferation ability of tongue squamous cell carcinoma cells

[0101] 1. Interference with ACTA1 gene expression

[0102] 1.1 siRNA synthesis

[0103] Design and synthesize siRNA sequence siRNA-ACTA1 according to the mRNA sequence of ACTA1 gene:

[0104] The sense strand is 5'-AUGAUCUUGAUCUUCAUGGUG-3' (SEQ ID NO.5);

[0105] The antisense strand is 5'-CCAUGAAGAUCAAGAUCAUCG-3'(SEQ ID NO.6),

[0106] The above siRNA sequences and the negative control siRNA sequence (siRNA-NC) (the negative control siRNA has no homology to the ACTA1 gene sequence) were provided by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0107] 1.2 Culture and transfection of tongue squamous cell carcinoma cells

[0108] Human tongue squamous carcinoma cell line HN4 was cultured in DMEM / F12 medium containing 10% FBS, 100 U / m L penicillin and 100 μg / m L streptomycin. All cells were placed in 5% CO 2 in a 37 °C cell culture inc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a genetic marker. The genetic marker is ACTA1. ACTA1 can be used for determining whether a subject has a risk of suffering from the tongue squamous cell carcinoma or not or determining whether the subject suffers from the tongue squamous cell carcinom or not. In addition, ACTA1 also can be used for preparing drugs for treatment of the tongue squamous cell carcinoma. The invention provides a new diagnostic method for clinical diagnosis of the tongue squamous cell carcinoma at the molecular level and further provides a new drug target for gene therapy of the tongue squamous cell carcinoma.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of ACTA1 gene in the diagnosis and treatment of squamous cell carcinoma of the tongue. Background technique [0002] Tongue cancer is one of the most common malignant tumors in the oral and maxillofacial region. Squamous cell carcinoma is the most common and ranks first among oral cancers. Tongue cancer mostly occurs on the side edge of the middle third of the tongue. It has a high degree of malignancy and strong local infiltration. It often involves the tongue muscle, restricts tongue movement, and affects speech, eating, and swallowing functions. Despite great progress in treatment, the survival rate of tongue cancer patients has not improved significantly in the past few decades. [0003] Current studies have shown that smoking, drinking and other chemical factors, human papillomavirus (human papillomavirus, HPV) and genetic factors are closely related to the occu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/574A61K31/713A61P35/00
CPCA61K31/713C12Q1/6886C12Q2600/118C12Q2600/158G01N33/57407G01N33/6887G01N2333/4712
Inventor 杨承刚杜海威
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com