Novel fermentation production method of saccharomycopsis fibuligera
A technology of buttoning capsule-coating and fermentation methods, which is applied in the field of fermentation and production of new button-coating-coating yeast, to achieve the effects of reducing the inactivation of live bacteria, low operation difficulty, and a large number of viable bacteria
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Embodiment 1
[0025] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. Starch 20g, water 1L;
[0026] (2) Seed preparation: first conduct conventional strain screening and activation, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 40 hours to obtain liquid seeds in the logarithmic phase;
[0027] (3) Preparation of solid plate: Add 24g of agar to each 1L of yeast liquid seed medium, sterilize it and pour it into the plate, and after cooling, it can be made into a solid plate medium containing liquid medium components ;
[0028] (4) Scale-up fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed fermentation and culture for 30 hours;
[0029] (5) Collecting the sludge: After the fermentation is over, take the...
Embodiment 2
[0032] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. The proportion of the medium was: yeast extract 8g, bacteriological peptone 22g, malt powder 22g, Starch 18g, water 1L;
[0033] (2) Seed preparation: first conduct conventional strain screening and activation, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 36 hours to obtain liquid seeds in the logarithmic phase;
[0034] (3) Preparation of solid plate: Add 18g of agar to every 1L of yeast liquid seed medium, sterilize it and pour it into the plate. After cooling, it can be made into a solid plate medium containing liquid medium components. ;
[0035] (4) Enlarged fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed fermentation and cul...
Embodiment 3
[0039] (1) Preparation of liquid seed medium: Through medium optimization experiments, the optimal liquid seed medium for cultivating denitrifying bacteria was obtained. The proportion of the medium was: yeast extract 12g, bacteriological peptone 18g, malt powder 18g, Starch 22g, water 1L;
[0040] (2) Seed preparation: perform conventional strain screening and activation first, then inoculate in the optimized liquid seed medium, and cultivate in the incubator for 48 hours to obtain liquid seeds in the logarithmic phase;
[0041] (3) Preparation of solid plate: Add 32g of agar to each 1L of liquid seed culture medium of Saccharomyces fumigatus, pour it into the plate after sterilization, and make a solid plate medium containing liquid medium components after cooling ;
[0042] (4) Scale-up fermentation: Spray the liquid seeds obtained in step (2) evenly on the surface of the solid plate medium, and then place it in a constant temperature fermentation room at 35°C for closed f...
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