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Use of aframomum melegueta and method for extracting and separating 6-shogaol from aframomum melegueta

A technology of shogaol and heavenly pepper, which is applied in the field of extraction and separation of 6-shogaol, can solve problems that have not been introduced yet, achieve low cost, high yield, and reduce the cost of raw materials

Inactive Publication Date: 2017-06-06
陕西嘉禾药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In the prior art, there is no report on the use of Paradise Pepper as an extraction of 6-shogaol, and there is no introduction that can be suitable for industrial production, and the method for extracting and separating 6-shogaol from Paradise Pepper

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1000 kg of raw materials were crushed to 60 mesh, and subcritical extraction was used to obtain oil extract, and the extraction residue was used for the extraction of 6-zingerol.

[0040] The above-mentioned extract was dissolved in 95% ethanol, and then extracted twice with a mixed solvent of n-hexane and petroleum ether in an equal amount. The volume ratio of n-hexane and petroleum ether was 1:1. The extracts were combined and concentrated to the extract.

[0041] The diameter of the pretreatment column is 10cm, and the height is 50cm. The filler used is aluminum oxide. The conventional column filler is silica gel and reverse phase chromatography silica gel C18, and its ratio is 1:1. The elution solvent is n-hexane:ethyl acetate=8: 1. Collect the eluate containing the active ingredient, concentrate to 1 / 100 of the eluate, and place it for crystallization.

[0042] After dissolving the above-mentioned crystalline sample in acetone with 6 times the crystalline mass, con...

Embodiment 2

[0044] 1000 kg of raw materials were pulverized to 100 mesh, and subcritical extraction was used to obtain oil extract, and the extraction residue was used for the extraction of 6-zingerol.

[0045] The above extract was dissolved with 95% ethanol, then extracted twice with a mixed solvent of petroleum ether and ethyl acetate with 1.5 times the mass of the extract, the volume ratio of petroleum ether and ethyl acetate was 10:1, the extracts were combined and concentrated to the extract.

[0046] The pretreatment column diameter is 20cm, and height is 50cm, and used filler is gac, and conventional column filler is chromatographic filler UniP MM500, and elution solvent is sherwood oil: ethyl acetate=4:1, collects the eluent containing active ingredient, Concentrate to 1 / 10 of the eluate and leave to crystallize.

[0047] The above-mentioned crystalline sample was dissolved in ethyl acetate with 10 times the crystalline mass, concentrated to 0.8 times the mass of the crystalline...

Embodiment 3

[0049] 1000kg of raw materials were pulverized to 80 mesh, and subcritical extraction was used to obtain oil extract, and the extraction residue was used for the extraction of 6-zingerol.

[0050] The above-mentioned extract is dissolved in 95% ethanol, then extracted twice with a mixed solvent of n-hexane, sherwood oil and ethyl acetate equal to the mass of the extract, and the volume ratio of n-hexane, sherwood oil and ethyl acetate is 8:5 : 1, the extracts are combined and concentrated to extract.

[0051] The pretreatment column diameter is 20cm, and height is 50cm, and used filler is Sephadex LH-200, and conventional column filler is chromatographic filler UniP MM500, and elution solvent is n-hexane: sherwood oil: ethanol=91:8:1, collects and contains effective The eluate of the components was concentrated to 5 / 100 of the eluate, and left to crystallize.

[0052]The above-mentioned crystalline sample was dissolved in ethanol with 7 times the crystalline quality, concentr...

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PUM

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Abstract

The invention provides a use of aframomum melegueta and a method for extracting and separating 6-shogaol from aframomum melegueta, and particularly relates to the use of aframomum melegueta for extraction of 6-shogaol. The method comprises the following steps: crushing the raw material aframomum melegueta, and adopting subcritical extraction, to obtain an oil extract; dissolving the oil extract by ethanol, then extracting by a solvent, and concentrating to an extract; adopting column separation, according to thin layer spots, collecting an eluate containing 6-shogaol, concentrating, and placing to crystallize; dissolving the crystal by an organic solvent, concentrating, then filtering the crystal, and drying to obtain 6-shogaol. The use of the aframomum melegueta for extraction of 6-shogaol is provided, the content of 6-shogaol in the aframomum melegueta raw material is multiple times higher than the content of 6-shogaol in a raw ginger raw material, the content of 6-shogaol in the aframomum melegueta raw material is 0.30%-0.42%, and the content of 6-shogaol in the raw ginger raw material is reported to be 0.005%; the obtained product has the advantages of high yield, stability and low cost.

Description

technical field [0001] The invention relates to the use of Paradise Pepper and a method for extracting and separating 6-shogaol from Paradise Pepper. Background technique [0002] Paradise pepper, scientific name Zingiberaceae is a perennial herb of the monocot class Zingiberaceae. It was once widely used as a substitute for pepper. Paradise pepper can be used as a condiment and can also treat cough, enteritis, bronchitis, syphilis, colds and rheumatism etc., and can be used as insecticide. [0003] Paradise Pepper is mainly used to extract 6-shogaol for weight loss or as a seasoning substitute, but there is no report on the extraction and separation of 6-shogaol from Paradise Pepper. [0004] Studies have reported that 6-shogaol has strong anti-oxidation, anti-inflammatory, anti-tumor, inhibition of cell proliferation, induction of apoptosis and inhibition of cancer cell invasion and other activities. [0005] At present, the research raw materials for 6-shogaol mainly co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C45/78C07C49/255
CPCC07C45/78C07C49/255Y02P20/54
Inventor 肖红王晓莹张瑜肖金霞郭文华
Owner 陕西嘉禾药业有限公司
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