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Zeranol detection method and kit in grains

A technology of zearalenol and zearalenol, which is applied in the field of detection, can solve the problems of undetectable and harmful effects of edible animals, reduce the operation steps and labor intensity, shorten the detection time, and overcome the problems of long detection cycle Effect

Inactive Publication Date: 2017-05-31
WUXI X RES PROD DESIGN & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, experiments have proved that it is harmful to mammals. In 1998, the European Union banned the use of zearalanol and other hormone drugs in livestock and poultry breeding. In 2002, the Ministry of Agriculture Announcement No. 193 clearly stipulated that zearalanol was prohibited All food animals, all edible animals shall not be detected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The method and kit for detecting zearalenol in grains of the present invention include a standard product of zearalenol, a standard product of β-zearalenol, a standard product of α-zearalenol, and a standard product of β-zearalenol , Zearalenone standard, zearalenone standard, biotinylated zearalenol, anti-mouse IgG coupled receptor magnetic beads, streptavidin donor magnetic beads, anti-zearalenol monogram Anti-antibody and detection buffer, wherein the anti-mouse IgG coupled receptor magnetic beads include 100μg / mL receptor magnetic beads, 0.05% Proclin-300 and pH7.4 PBS solution; streptavidin donor magnetic beads Including 100μg / mL donor magnetic beads, 25mM HEPES, 100mM NaCl solution and 0.1% Proclin-300; Weigh 1g agarose gel matrix powder, dissolve it in 1mmol / L HCl, and place it in a sintered glass filter Wash with 1mmol / L HCl for 15 minutes; for ligand coupling, use coupling buffer 0.2mol / L NaHCO3pH8.3 to dissolve the β-zearalenol antibody and chloramphenicol anti...

Embodiment 2

[0013] Methods and kits for the detection of zearalenol in grains, including standard zearalenol, β-zearalenol standard, α-zearalenol standard, β-zearalenol standard, and corn Gibberellone standard, zearalenone standard, biotinylated zearalenol, anti-mouse IgG coupled receptor magnetic beads, streptavidin donor magnetic beads, anti-zearalenol monoclonal antibody And detection buffer, wherein the anti-mouse IgG-coupled acceptor magnetic beads include 100μg / mL acceptor magnetic beads, 0.05% Proclin-300 and pH7.4 PBS solution; streptavidin donor magnetic beads include 100μg / mL donor magnetic beads, 25mM HEPES, 100mM NaCl solution and 0.2% Proclin-300; Weigh 1g agarose gel matrix powder, dissolve it in 1mmol / L HCl, and then place it in a sintered glass filter and use 1mmol / LHCl wash for 15min; ligand coupling use coupling buffer 0.2mol / LNaHCO3pH8.3 to dissolve the β-zearalenol antibody and chloramphenicol antibody to be coupled to obtain the antibody solution, and quickly transfe...

Embodiment 3

[0015] The method and kit for detecting zearalenol in grains of the present invention include a standard product of zearalenol, a standard product of β-zearalenol, a standard product of α-zearalenol, and a standard product of β-zearalenol , Zearalenone standard, zearalenone standard, biotinylated zearalenol, anti-mouse IgG coupled receptor magnetic beads, streptavidin donor magnetic beads, anti-zearalenol monogram Anti-antibody and detection buffer, wherein the anti-mouse IgG-coupled acceptor magnetic beads include 100μg / mL acceptor magnetic beads, 0.05% Proclin-300 and pH7.4 PBS solution; streptavidin donor magnetic beads Including 100μg / mL donor magnetic beads, 25mM HEPES, 100mM NaCl solution and 0.15% Proclin-300; Weigh 1g agarose gel matrix powder, dissolve it in 1mmol / L HCl, and place it in a sintered glass filter Wash with 1mmol / L HCl for 15min; use coupling buffer 0.2mol / LNaHCO3pH8.3 to dissolve the β-zearalenol antibody and chloramphenicol antibody to be coupled to obta...

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Abstract

The invention discloses a zeranol detection method and kitfor grains. The zeranol detection method comprises a zeranol standard substance, a beta-zeranol standard substance, an alpha-zearalenol standard substance, a beta-zearalenol standard substance, a zearalanone standard substance, a zearalenone standard substance, biotinylated zeranol, anti-mouse IgG coupled receptor magnetic beads, streptavidin donor magnetic beads, an anti-zeranol monoclonal antibody and a detection buffer solution. The anti-mouse IgG coupled receptor magnetic beads comprise 100 mu g / ml receptor magnetic beads, 0.05%Proclin-300 and a PBS (phosphate buffer solution) with the pH value of 7.4. The streptavidin donor magnetic beads comprise 100 mu g / ml donor magnetic beads, 25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), a 100 mM NaCl solution and 0.1-0.2% Proclin-300. The method can overcome the defects of long detection period, complex steps, time consuming, labor consuming and the like in the traditional detection process, greatly shortens the detection time, greatly reduces the operation steps and labor intensity, and satisfies the requirements of time saving, labor saving, high speed and high sensitivity.

Description

Technical field [0001] The invention relates to a detection method, in particular to a detection method and a kit for zearalenol in grains. Background technique [0002] Zearalenol, also known as "Right Cyclotetradecanol", is a reduction product of zearalenone, a secondary metabolite produced by Gibberella zeae during the growth process, and belongs to the non-steroidal class of rasonic acid lactones. Anabolic hormones. Zearalenone and its metabolites—α-zearalenol, β-zearalenol, zearalenol, zearalenol and β-zearalenol may contaminate feed and cause animal products Zearalenol residues were detected in Zearalenol is an ideal skin-buried weighting agent, which is a non-sterol and non-hormonal compound. However, experiments have proved that it is harmful to mammals. In 1998, the European Union banned the use of zearalenol and other hormone drugs in livestock and poultry breeding. In 2002, the Ministry of Agriculture Announcement No. 193 clearly stipulated that zearalenol was prohi...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N33/74
Inventor 徐静赵春城胡勇蒋韦艳刘金杰吴敏芳朱倩倩
Owner WUXI X RES PROD DESIGN & RES
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