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Method for detecting glucose through nanoscale molybdenum oxide mimic enzyme based on paper lab on a chip

A technology of microfluidic chips and glucose, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators, etc., which can solve the problems of high cost, difficult on-site real-time detection, and slow analysis speed, etc. question

Inactive Publication Date: 2017-05-31
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these technologies have certain limitations such as slow analysis speed, high cost, and difficulty in on-site instant detection.

Method used

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  • Method for detecting glucose through nanoscale molybdenum oxide mimic enzyme based on paper lab on a chip
  • Method for detecting glucose through nanoscale molybdenum oxide mimic enzyme based on paper lab on a chip
  • Method for detecting glucose through nanoscale molybdenum oxide mimic enzyme based on paper lab on a chip

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Experimental program
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Embodiment 1

[0038] A method for detecting glucose with a paper microfluidic chip-based nanomolybdenum oxide mimic enzyme is as follows:

[0039] (1) Synthesis of MoOx quantum dots

[0040] Weigh 0.03 g MoS 2 , add 200 mL of deionized water at 65 °C, continue ultrasonication for 17 hours, and then filter with a 0.22 µm microporous membrane to collect the filter cake; then dissolve the product in 30 mL of deionized water, and react under hydrothermal reaction at 200 °C for 12 hours . After cooling naturally, filter with suction and collect the filtrate to obtain a molybdenum oxide quantum dot (MoOxQDs) solution.

[0041](2) Glucose oxidase reacts with glucose

[0042] Prepare a range of concentrations of glucose standard solutions. Add 1.25U glucose oxidase to glucose standard solutions of different concentrations, and react with the enzyme for 15 minutes at room temperature to obtain the required H 2 o 2 solution.

[0043] (3) Glucose determination by paper microfluidic chip

[004...

Embodiment 2

[0047] A method for detecting glucose with a paper microfluidic chip-based nanomolybdenum oxide mimic enzyme is as follows:

[0048] Take a certain amount of serum sample in an ultrafiltration tube, centrifuge at 10,000 rpm for 10 min to remove macromolecular substances in the serum, take the analysis solution after centrifugation, add 1.25U glucose oxidase to the serum analysis solution of different concentrations, and react with the enzyme at room temperature for 15 minutes After that, get the required H 2 o 2 solution, coated with H 2 o 2 solution on a paper microfluidic chip, making MoOx QDs-ABTS-H 2 o 2 The color development system is integrated on the paper microfluidic chip to react, and the image of color change is collected by taking pictures, and the gray value data of the color change is processed by ImageJ software to detect glucose.

[0049] Such as image 3 shown at 1.3mM ~ In the range of 20mM, the serum glucose concentration has a good linear correlatio...

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Abstract

The invention discloses a method for detecting glucose through ananoscale molybdenum oxide mimic enzyme based on a paper lab on a chip. The method comprises the steps of utilizing excellent characteristics of a molybdenum oxide quantum dot mimic enzyme; combining a paper lab on a chip technology, and specifically catalytic reaction on the glucose through glucose oxidase; integrating a MoOx QDs-ABTS-H2O2 developing system on the chip for reacting; carrying out data processing on a developing result through scanner imaging and Adobe-Photoshop software; building a fast, simple, convenient, economical and accurate glucose detection paper-base lab on a chip new method for quantitatively analyzing the glucose in serum. The method can provide a scientific experiment basis for POCT (Point Of Care Testing) detection product development for diabetic blood sugar concentration, and has significance on rapid diagnosis of diabetes, treatment evaluation, prognosis and in-time disease development control.

Description

technical field [0001] The invention relates to a method for detecting glucose with a paper microfluidic chip-based nanomolybdenum oxide imitating enzyme, belongs to the technical field of detection, and relates to a microfluidic chip used for detecting glucose in serum. Background technique [0002] Microfluidic chip technology (Lab on a Chip) integrates various functions of the laboratory on a chip of a few square centimeters. It has multi-disciplinary and multi-field interdisciplinary functions and is a comprehensive laboratory that can be carried around. In recent years, with the development of science and technology, microfluidic chip technology has become an important analysis and detection tool. Paper-based microfluidic chips have the advantages of low cost, strong operability, good biocompatibility, miniaturization, and portable on-site rapid detection. They will have great potential in the fields of rapid medical diagnosis, rapid detection of food safety, and rapid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 刘爱林彭花萍胡祥光李珊红连芯雷云陈伟林新华
Owner FUJIAN MEDICAL UNIV
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