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Aptamer based method for detecting surface enhanced raman spectroscopy of tetracycline residue in milk

A surface-enhanced Raman and spectral detection technology, which is applied in Raman scattering, measuring devices, and material analysis through optical means, to achieve high sensitivity, excellent biocompatibility, and improved stability and repeatability

Active Publication Date: 2017-05-31
JIANGSU UNIV
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Problems solved by technology

[0003] At present, gold nanoparticles / silica core-shell composite nanomaterials optimized to modify p-mercaptoaniline-labeled molecules are used as surface-enhanced Raman substrates, combined with nucleic acid aptamers that can specifically bind to the target tetracycline to be detected. Sensitive and effective detection of tetracycline has not been reported

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  • Aptamer based method for detecting surface enhanced raman spectroscopy of tetracycline residue in milk
  • Aptamer based method for detecting surface enhanced raman spectroscopy of tetracycline residue in milk

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Embodiment 1

[0026]In order to further verify the effect of the detection method prepared by the present invention on the detection of tetracycline residues in milk, examples of the present invention, taking tetracycline (tetracycline) as an example, the specific operation steps are as follows:

[0027] (1) Preparation of magnetic microspheres: Synthesize magnetic microspheres by solvothermal method. First, weigh 1.350g FeCl 3 .6H 2 O, 3.854g of sodium acetate and 0.400g of sodium citrate were dissolved in a 250mL three-neck round-bottomed flask filled with 70mL of ethylene glycol. Subsequently, the above solution was heated to 300°C under the protection of argon, and the reaction was continued for 1h to form a uniform Black-red solution. After the solution was cooled to room temperature, it was transferred to a 100 mL polytetrafluoroethylene-lined stainless steel reactor, sealed and heated at 200°C for 17 hours. Finally, by magnetic separation and collection, wash with ethanol and ultra...

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Abstract

The invention relates to an aptamer based method for detecting surface enhanced raman spectroscopy of a tetracycline residue in milk. The method is as follows: a prepared gold nanoparticle / silicon dioxide nuclear shell composite nanomaterial modifying a p-aminothiophenol labelled molecule is taken as a surface enhanced raman base, and an adding standard recovery measurement mode is used to realize the detection and study of the tetracycline residue in the milk. According to the method for detecting the surface enhanced raman spectroscopy of the tetracycline in the milk, the existence of the tetracycline affects the structure of an aptamer in a detection system, the tetracycline and the aptamer are specifically identified and combined, so that the enhanced bases at different concentrations are released under the existence of the tetracycline at different concentrations, then the detection system is magnetically separated, a composite containing a magnetic microsphere, the aptamer and the tetracycline is adsorbed to the bottom, supernates containing the enhanced bases at different concentrations are subjected to raman signal measurement, and the purpose of sensitively and indirectly detecting the tetracycline residue in the milk is realized according to the characteristic peak map of the labelled molecule at different raman intensities.

Description

technical field [0001] The invention relates to an aptamer-based detection method for surface-enhanced Raman spectroscopy of tetracycline residues in milk, specifically preparing gold nanoparticles / silica core-shell composite nanomaterials modified with p-mercaptoaniline-labeled molecules as surface-enhanced Raman substrates , combined with the nucleic acid aptamer that can specifically bind to the target substance tetracycline to be detected, and finally realize the rapid, sensitive and efficient detection research of tetracycline residues in milk by means of standard addition recovery measurement. Background technique [0002] Tetracyclines Tetracyclines are widely used as feed additives in animal husbandry to prevent and treat intestinal infection and promote growth in animals. In the dairy farming industry, due to the abuse of tetracycline and non-compliance with the withdrawal period regulations, it can accumulate in the liver and kidney tissues and be excreted with mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李欢欢陈全胜赵杰文欧阳琴郭志明林颢刘妍孙浩杨明秀
Owner JIANGSU UNIV
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