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A strain fermentation method and application of microbial fertilizer

A technology of microbial fertilizer and fermentation method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to breed vitality and affect the performance of fertilizer and water agents, and achieve good fertilizer and water effects

Active Publication Date: 2021-01-19
SHANDONG XINGSHENG MINING IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this fertilizer and water agent, the fermentation of microbial strains adopts a conventional fermentation method, which cannot maximize the vitality of the bacteria, and also affects the performance of the fertilizer and water agent to a certain extent.

Method used

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  • A strain fermentation method and application of microbial fertilizer
  • A strain fermentation method and application of microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Composite fermentation bacterium liquid described in the present embodiment is obtained according to the following method:

[0036] (1) respectively inoculate the preserved Lactobacillus casei, Bacillus amyloliquefaciens, Penicillium strains, and Pichia membranoidis into their respective suitable seed liquid culture medium to carry out aseptic culture activation to obtain the seed activation liquid of each bacterial strain;

[0037] (2) Preparation of fermentation broth: take 30g of glucose, 30g of yeast extract, NaH 2 PO 4 2g, MgSO 4 0.6g, 1L of water, and adjust the pH to 7.0; take 100mL of high-temperature sterilized fermentation medium, inoculate 1mL of the seed activation solution of each bacterial strain obtained above, and add 0.6g of L-alanine ionic liquid to mix well, and Control 28-30 ℃ to ferment, that is.

Embodiment 2

[0039] Composite fermentation bacterium liquid described in the present embodiment is obtained according to the following method:

[0040] (1) respectively inoculate the preserved Lactobacillus casei, Bacillus amyloliquefaciens, Penicillium strains, and Pichia membranoidis into their respective suitable seed liquid culture medium to carry out aseptic culture activation to obtain the seed activation liquid of each bacterial strain;

[0041] (2) Preparation of fermentation broth: get 50g of sucrose, 20g of peptone, Na 2 HPO 4 4g, MgCl 2 0.4g, 1L of water, and adjust the pH to 7.0; take 100mL of high-temperature sterilized fermentation medium, inoculate 1mL of the seed activation solution of each bacterial strain obtained above, and add 1.2g of L-phenylalanine ionic liquid to mix evenly. And control 28-30 ℃ for fermentation, that is.

Embodiment 3

[0043] Composite fermentation bacterium liquid described in the present embodiment is obtained according to the following method:

[0044] (1) respectively inoculate the preserved Lactobacillus casei, Bacillus amyloliquefaciens, Penicillium strains, and Pichia membranoidis into their respective suitable seed liquid culture medium to carry out aseptic culture activation to obtain the seed activation liquid of each bacterial strain;

[0045] (2) Preparation of fermentation medium: take molasses 40g, beef extract 25g, Na 2 HPO 4 3g, MgSO 4 0.5g, 1L of water, and adjust the pH to 7.0; take 100mL of high-temperature sterilized fermentation medium, inoculate 1mL of the seed activation solution of each bacterial strain obtained above, and add 0.8g of L-proline ionic liquid to mix evenly, and Control 28-30 ℃ to ferment, that is.

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Abstract

The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain fermentation method of microbial fertilizer, and application of the strain fermentation method. According to the strain fermentation method of the microbial fertilizer, a strain suitable for fertilizing water is selected, and amino acid ionic liquid is added based on the conventional fermentation way for activating the strain, so that the obtained compound fermentation liquor has a better water fertilizing effect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain fermentation method of microbial fertilizer and its application. Background technique [0002] At present, with the development of the economy and the improvement of people's living standards, more and more aquatic products have entered thousands of households, and the corresponding scale of aquaculture has also become larger and larger, and has formed an industrial scale in our country, occupying an important share in the national economy. important position. [0003] In the whole process of aquaculture industry, with the consumption of nutrients in the water and the discharge of waste from the aquaculture products, the fertilization of the aquaculture water (also known as fertilizer water) is a very critical step, which not only occupies a very important position and directly affects Farming production. Generally, fertilizer and aquatic pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F17/20C05F17/50C05G3/00C05G3/80C12R1/245C12R1/07C12R1/80C12R1/84
CPCC05F5/002C05F17/00C05G3/00C12N1/14C12N1/16C12N1/20C05G3/80C05F11/00C05F11/02C05F11/08Y02W30/40
Inventor 李运德
Owner SHANDONG XINGSHENG MINING IND
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