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Streptomyces lydicus for preventing and curing fusarium wilt and application thereof

A technology for Streptomyces lydidea and Fusarium wilt, which is applied in the field of Streptomyces lydidee for the prevention and treatment of Fusarium wilt, can solve problems such as environmental pollution, excessive pesticide residues in melon fruits, and impact on people's safety and health, achieving obvious antagonistic effects and broad application prospects Effect

Inactive Publication Date: 2017-05-31
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and treatment of Fusarium wilt of melon is mainly based on chemical agents. Although chemical control can quickly eliminate pathogenic bacteria, it is easy to cause drug resistance of melon plants, pesticide residues in melon fruits exceed the standard, and the environment is polluted, which in turn affects people's safety and health.

Method used

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  • Streptomyces lydicus for preventing and curing fusarium wilt and application thereof
  • Streptomyces lydicus for preventing and curing fusarium wilt and application thereof
  • Streptomyces lydicus for preventing and curing fusarium wilt and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: the activation culture of Streptomyces lydidii

[0018] After cultivating Streptomyces lydidii for 5, 7, and 10 days by the insert method, observe the aerial hyphae, sporocydia, sporocydia, and spore shapes ( figure 1 ): The strain forms aerial hyphae and spore filaments, the sporocysts are helical, and the buns are oval or elliptical.

[0019] Inoculate Streptomyces lydidae on Gao's No. 1 agar, glycerol asparagine agar, inorganic salt starch agar, yeast malt extract agar, oat flour agar, sucrose nitrate agar, glucose-asparagine agar, tyrosine agar, etc. On the medium, after culturing at 28°C for 7 days, the culture characteristics on various mediums were observed, see Table 1 for details. Detect its physiological and biochemical characteristics, see Table 2 for details.

[0020] Table 1 The characteristics of Streptomyces lydidii on different media

[0021]

[0022] Table 2 Physiological and biochemical characteristics of Streptomyces lydidis strain...

Embodiment 2

[0026] Example 2: Methylotrophic bacillus antagonism experiment on pathogenic fungi

[0027] Plate stand-off experiment: with melon wilt fungus FJAT-9062 and FJAT-9230 as indicator bacteria, the bacterial strains stored in the -4°C environment were punched out with a puncher to get 5 mm bacterial cakes and then placed on PDA medium (potato 200g , glucose 20g, agar 15g, deionized water 1000ml) around the pathogenic bacteria flat plate of 3d cultivation, spot junction is apart from pathogenic bacteria center 2cm, each flat plate points 4 places, and measures its antagonistic index after cultivating 4d. Statistical data show that the antagonistic index of Streptomyces lydidae to melon wilt FJAT-9062 and FJAT-9230 are 3.46 and 2.41 respectively, and the antagonistic effects are shown in figure 2 .

[0028] Antagonism index is a way of expressing the antagonism effect of antagonistic bacteria on pathogenic bacteria. The larger the antagonism index, the better the antagonism effec...

Embodiment 3

[0029] Embodiment 3: Methylotrophic bacillus genetic stability experiment

[0030] According to the method of plate confrontation experiment, the subculture experiment was carried out on Streptomyces lydidii, and the changes of its antagonistic index were observed generation by generation, so as to determine the genetic stability of the strain. In this experiment, the first generation, fifth generation, tenth generation, fifteenth generation and twentieth generation of Streptomyces lydidis were measured, and the results are shown in image 3 . Depend on image 3 It can be seen that methylotrophic Bacillus still has antagonistic effects on melon wilt FJAT-9062 and FJAT-9230 in the twentieth generation, which explains to a certain extent that Streptomyces lydidis has an inhibitory effect on melon wilt FJAT-9062 and FJAT-9230. The antagonistic effect of 9230 is relatively long-lasting, which is beneficial for the bacterium to inhibit the pathogenic bacterium for a long time.

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Abstract

The invention relates to the technical field of microbial fertilizers, particularly to Streptomyces lydicus for preventing and curing fusarium wilt and application thereof. A strain is classified and named as Streptomyces lydicus, the Streptomyces lydicus is preserved in CGMCC (China general microbiological culture collection) located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing City on October 12, 2016, and the preservation No. is CGMCC No.13106. The Streptomyces lydicus provided by the invention has an obvious antagonistic effect on fusarium wilt of melons, is a biocontrol strain with wide application prospect and can serve as a functional strain for preparing the microbial fertilizers.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a strain of Streptomyces lydida for preventing and treating Fusarium wilt and its application. Background technique [0002] Melon is one of the top ten fruits in the world. Its fruit is delicious and sweet, and is deeply loved by people from all over the world. With the continuous increase of muskmelon planting area and planting density, while the production of muskmelon is gradually increasing, it is also facing the danger of increasing diseases and insect pests. Among them, muskmelon wilt is one of the most serious diseases affecting the production of muskmelon. Melon wilt is a soil-borne disease, which was first discovered in South Carolina, USA in 1894, and then various degrees of wilt have appeared in various parts of the world. In 1949, the production of melons in Florida, USA was reduced by more than 40% due to Fusarium wilt (Booth, C. Fusarium: laboratory ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 王进闯邹立飞余小兰李勤奋邹雨坤严廷良程汉亭杨霞
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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