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A method for extracting chenodeoxycholic acid

A technology of chenodeoxycholic acid and bile acid, which is applied in the field of bioengineering, can solve the problems of slow washing and removal speed, large loss of active ingredients, waste of biological resources, etc., and achieve the effects of high value, reduced loss, and improved extraction rate

Active Publication Date: 2018-08-17
CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the conventional method for clearing chenodeoxycholic acid is the washing method. Although this method is simple to operate, the washing and removal speed is slow, the efficiency is low, the loss of active ingredients is large, and a large amount of water phase is produced to be disposed.
Moreover, the active ingredients will also be lost with the water phase, resulting in waste of biological resources, which will bring great pressure on production costs and environmental protection sewage treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting chenodeoxycholic acid, comprising the steps of:

[0031] (1) Preparation of bile acid salts: get 320 kilograms of bile acid raw materials obtained by extracting chicken bile paste, saponify, adopt cooling water to cool the solution obtained after saponification to room temperature, then continue to open the cooling water, and adopt a mass percent concentration of 10% Adjust the pH of the solution to 6.5 with dilute hydrochloric acid to obtain a bile salt solution; wherein, when carrying out the saponification reaction, 320 kilograms of bile acid raw materials obtained by extracting chicken gall paste are put into a 5000L stainless steel dissolution kettle, and 1600 kilograms of water are added, while stirring. Add 64 kg of sodium hydroxide and heat to boiling. After the bile acid raw material and sodium hydroxide are fully dissolved, saponify at 90° C. for 5 hours under normal pressure; after saponification, the pH of the obtained solution is 11. ...

Embodiment 2

[0040] A method for extracting chenodeoxycholic acid, comprising the steps of:

[0041] (1) Preparation of bile salts: get 310 kilograms of bile acid raw materials obtained by extracting chicken bile paste, saponify, adopt cooling water to cool the solution obtained after saponification to room temperature, then continue to open the cooling water, and adopt a mass percentage concentration of 10% Adjust the pH of the solution to 6.6 with dilute hydrochloric acid to obtain a bile salt solution; wherein, when carrying out the saponification reaction, 310 kilograms of bile acid raw materials obtained by extracting chicken bile paste are put into a 5000L stainless steel dissolution kettle, and 1550 kilograms of water are added, while stirring. Add 62 kg of sodium hydroxide and heat to boiling. After the bile acid raw material and sodium hydroxide are fully dissolved, saponify at 90°C for 4 hours under normal pressure; after saponification, the pH of the obtained solution is 12.

[...

Embodiment 3

[0050] A method for extracting chenodeoxycholic acid, comprising the steps of:

[0051] (1) Preparation of bile acid salts: get 335 kilograms of bile acid raw materials obtained by extracting chicken bile paste, saponify, adopt cooling water to cool the solution obtained after saponification to room temperature, then continue to open the cooling water, and adopt a mass percentage concentration of 10% Adjust the pH of the solution to 6.4 with dilute hydrochloric acid to obtain a bile salt solution; wherein, when carrying out the saponification reaction, 335 kilograms of bile acid raw materials obtained by extracting chicken bile paste are put into a 5000L stainless steel dissolution kettle, and 1675 kilograms of water are added, while stirring. Add 67 kg of sodium hydroxide and heat to boiling. After the bile acid raw material and sodium hydroxide are fully dissolved, saponify at 95° C. for 3 hours under normal pressure; after saponification, the pH of the obtained solution is 1...

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Abstract

The invention discloses a method for extracting chenodeoxycholic acid, and belongs to the technical field of bioengineering. The method comprises the following steps of (1) preparing bile acid sodium salt; (2) preparing bile acid amine salt; (3) extracting and separating a bile acid amine salt solution; (4) salting out and carrying out alkaline hydrolysis. According to the method, when the chenodeoxycholic acid is extracted, chenodeoxycholic acid amine salt and cholic acid amine salt regulated through organic acid in a butyl ester solution have a remarkable solubility difference, so that the chenodeoxycholic acid amine salt and the cholic acid amine salt are crude-separated, and the content of cholic acid is reduced to below 3 percent through crude separation; the chenodeoxycholic acid amine salt and the cholic acid amine salt have a larger solubility difference in a high-concentration butyl ester solvent and a dichloromethane water solution system, and the cholic acid is cleaned and treated again, so that the chenodeoxycholic acid is refined and purified, the content of the cholic acid is reduced to be within 0.2 percent, and the extraction ratio of the chenodeoxycholic acid is greatly improved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting chenodeoxycholic acid. Background technique [0002] Chenodeoxycholic acid in bile acids has the function of reducing the saturation of cholesterol in bile, so it has a good therapeutic effect on gallbladder stone disease. And chenodeoxycholic acid is the raw material for preparing ursodeoxycholic acid, and the content of cholic acid in chenodeoxycholic acid is generally greater than 5%, which is the main impurity in chenodeoxycholic acid, which will definitely affect the quality of chenodeoxycholic acid. Therefore, extracting chenodeoxycholic acid from bile acids and further removing cholic acid is an important step for purifying chenodeoxycholic acid. [0003] At present, the conventional method for clearing chenodeoxycholic acid is the washing method. Although this method is simple to operate, the washing and removal speed is slow, the efficienc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 邓家国雷平
Owner CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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