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Colorimetric fluorescence probe for quickly and highly selectively analyzing cupric ions and preparation method thereof

A technology for fluorescein and preparations, which is applied in the fields of analyzing materials, fluorescence/phosphorescence, and material excitation analysis, and can solve problems such as difficulties in copper ion detection, and achieve the effects of being conducive to popularization and application, good stability, and simple synthesis

Inactive Publication Date: 2017-05-31
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The appropriate concentration of copper ions in drinking water is 0.5-1.0 mg / L, which brings great difficulties to the detection of copper ions. Therefore, it is urgent to develop analytical methods that can detect copper ions in living bodies with high sensitivity.

Method used

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  • Colorimetric fluorescence probe for quickly and highly selectively analyzing cupric ions and preparation method thereof
  • Colorimetric fluorescence probe for quickly and highly selectively analyzing cupric ions and preparation method thereof
  • Colorimetric fluorescence probe for quickly and highly selectively analyzing cupric ions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]

[0034] (Scheme 1) Dissolve 387mg (1mmol) of rhodamine B hybridized fluorescein in 15mL of dichloromethane, then add 246mg (2mmol) of 2-pyridinecarboxylic acid to reflux for 6h, then use a vacuum pump to carry out suction filtration to obtain the filtrate, and the filtrate Pass directly through the chromatographic column to obtain pure product. 295.2 mg of a light yellow pure product was obtained with a yield of 60%.

[0035] (Scheme 2) Dissolve 387mg (1mmol) of rhodamine B hybridized fluorescein in 15mL of dichloromethane, then add 307.5mg (2.5mmol) of 2-pyridinecarboxylic acid to reflux for 6h, then use a vacuum pump to perform suction filtration to obtain the filtrate, Pass the filtrate directly through the chromatographic column to obtain pure product. 310 mg of a light yellow pure product was obtained with a yield of 63%.

[0036] (Scheme 3) Dissolve 387mg (1mmol) of rhodamine B hybridized fluorescein in 15mL of dichloromethane, then add 369mg (3mmol) of 2-py...

Embodiment 2

[0041] Figure 1(a) is the probe (5μM) added Cu 2+ Fluorescence spectra before and after (5 μM). It can be clearly seen from the figure that the addition of copper ions leads to enhanced fluorescence intensity.

[0042] Figure 1(b) is the probe (5μM) added Cu 2+ Absorption spectra before and after (5 μM). It can be clearly seen from the figure that the addition of copper ions leads to an increase in the absorption value, and the color of the solution turns pink, so the naked-eye colorimetric analysis of copper ions can be realized by using this probe.

Embodiment 3

[0044] figure 2 are different concentrations of Cu 2+ (0-3μM) on the probe (5μM) fluorescence spectrum;

[0045] It can be seen from the figure that with the Cu in the probe solution 2+ As the concentration increased, the fluorescence intensity gradually increased. Importantly, the fluorescence intensity has a good linear relationship with the concentration of added copper ions, which proves that the fluorescent probe can be used for quantitative analysis of copper ions with high sensitivity.

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Abstract

The invention relates to a colorimetric fluorescence probe for quickly and highly selectively analyzing cupric ions and a preparation method thereof. Specifically, the probe is a 2-picolinate compound, and can serve as a cupric ion colorimetric fluorescence probe used for detection of cupric ions. The probe can realize at least one of the following technical effects: the cupric ions are highly selectively recognized; response for the cupric ions can be quickly realized; highly-sensitive analysis for the cupric ions can be realized; naked eye colorimetric analysis for the cupric ions can be realized; the characteristics are stable, and the cupric ions can be stored and used for long time; and the probe has relatively high anti-jamming capability.

Description

technical field [0001] The invention relates to a 2-pyridine carboxylate compound as a colorimetric fluorescent probe for copper ions, which can rapidly and sensitively identify copper ions with high selectivity, or it can measure the concentration of copper ions in a sample. Background technique [0002] Copper, the third most abundant transition metal, plays a pivotal role in cellular energy production, oxygen transport and activation, and signal transduction in the human body. Copper ion is a specific metal cation, which can be used as a catalytic cofactor of various metalloenzymes, such as tyrosinase, superoxide dismutase, cytochrome oxidase, lysyl oxidase, etc. plays a very crucial role. Although the content of copper in the human body is very low, the lack of copper can lead to the disorder of the body's metabolism and growth. Excessive copper content can also have a great toxic effect on the human body. If the balance of copper metabolism in the human body is disrup...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/10C09K11/06G01N21/64
CPCC07D493/10C09K11/06C09K2211/1029C09K2211/1088G01N21/6428G01N21/643G01N21/6447G01N21/6486
Inventor 段庆霞吕小钰柳彩云王作凯朱宝存杜斌
Owner UNIV OF JINAN
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