Combined microbial organic fertilizer for controlling grape sour rot and preparation method thereof
A technology of microbial organic fertilizer and grape acid is applied in the field of combined microbial organic fertilizer for preventing and controlling grape acid rot and its preparation. The effect of rupture, reducing the source of infection
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Embodiment 1
[0025] This embodiment relates to the preparation method of organic fertilizer, including fermentation, hydrolysis, separation, concentration / drying, compounding steps, specifically as follows:
[0026] 1) With glucose 1.0%, peanut powder 1.0%, corn flour 1%, sucrose 5%, MgSO 4 ·7H 2 O 0.1%, KH 2 PO 4 0.2% is the fermentation substrate, with Gibberella as the fermentation strain, 28°C ± 1°C for 155h, and the online monitoring of the concentration of gibberellin in the fermented species reaches 65ppm and stops.
[0027] 2) After the fermentation, add α-amylase to 1 mg / L to hydrolyze the residual polysaccharides in the fermentation broth for 5 hours, then add papain to 2 mg / L to fully hydrolyze the protein in the fermentation broth for 5 hours.
[0028] 3) After the hydrolysis is completed, settle for 24 hours. After the fermentation broth is stratified, extract the supernatant and concentrate it with a scraper to 1 / 10 of the original volume to obtain a fully water-soluble or...
Embodiment 2
[0034] Present embodiment relates to the implementation method of above-mentioned organic fertilizer, comprises:
[0035] 1) The application method of solid organic fertilizer is: when the base fertilizer is applied to the roots of grape pruning in autumn, it is directly applied to the soil instead of traditional farmyard manure and chemical fertilizer. The dosage is 100kg / mu.
[0036] 2) In this example, after applying solid organic fertilizer and fully water-soluble organic foliar fertilizer to the Lvjiang Grape Cooperative in Daqiao Town, Jiaxing City, Zhejiang Province, after using the solid organic fertilizer for 60 days in November 2015, the number of acetic acid bacteria in the soil was detected to be 5× 10 6 individual / g, compared with the control group 250×10 6per g, the base of sour rot pathogenic bacteria was greatly reduced.
[0037] 3) When using fully water-soluble organic foliar fertilizer, add water to dilute it 80 times, and spray it twice during the grape ...
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