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Targeted adriamycin-loaded magnetic nanoparticles and preparation method and application

A magnetic nanoparticle, targeted modification technology, applied in the field of anti-tumor photothermal therapy, can solve the problem of lack of specific targeting, achieve good target recognition ability, excellent photothermal killing function, good magnetic resonance imaging effect of function

Inactive Publication Date: 2017-05-31
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention provides a targeted modified magnetic nanoparticle loaded with doxorubicin and its preparation method and application to solve the problem of Fe 3 o 4 @PDA nanoparticles have no specific targeting problems when applied to magnetic resonance imaging and photothermal therapy of colon cancer. It provides a kind of EGFR antibody as a targeting group, which can specifically target colon cancer cells and has Multifunctional Fe for magnetic resonance imaging, photothermal therapy, and chemotherapy 3 o 4 @PDA-PEG-EGFR-DOX nanoparticles and their preparation methods and applications, and their nanobiological effects at the cell and animal levels, in order to contribute to the diagnosis and treatment of colon cancer

Method used

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  • Targeted adriamycin-loaded magnetic nanoparticles and preparation method and application
  • Targeted adriamycin-loaded magnetic nanoparticles and preparation method and application
  • Targeted adriamycin-loaded magnetic nanoparticles and preparation method and application

Examples

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Effect test

Embodiment 1

[0037] A kind of target-modified magnetic nanoparticles loaded with doxorubicin (Fe 3 o 4 @PDA-PEG-EGFR-DOX) preparation method, comprises the following steps:

[0038] (1) 2mmol of FeCl 3 Dissolve in a mixed solution of 4mL ethylene glycol and 16mL monocarboxydiethylene glycol, stir for 0.5h, add 2g of polyvinylpyrrolidone to the above solution, heat the solution to 120°C to obtain a transparent yellow solution, after 1h, stop Heat and add 1.5g of sodium acetate, continue to stir for 0.5h, transfer the solution to a 25mL reactor, seal the reactor and heat it to 200°C, continue the reaction for 12h, stop heating and cool the reactor to room temperature to obtain Fe 3 o 4 The nanoclusters were washed three times with ethanol and water, and dissolved in Tris buffer;

[0039] (2) the Fe-containing material prepared in step (1) 3 o 4 The Tris buffer of the nanoclusters was adjusted to pH 8.5, and 0.03M dopamine solution was slowly added, reacted at room temperature for 3 ho...

Embodiment 2

[0044] Include the following steps:

[0045] (1) 2mmol of FeCl 3 Dissolve in a mixed solution of 4mL ethylene glycol and 16mL monocarboxydiethylene glycol, stir for 0.75h, add 2g of polyvinylpyrrolidone to the above solution, and heat the solution to 120°C to obtain a transparent yellow solution. After 1.5h, Stop heating and add 1.5g sodium acetate, continue to stir for 0.75h, transfer the solution to a 25mL reactor, seal the reactor and heat it to 200°C, continue the reaction for 14h, stop heating and cool the reactor to room temperature to obtain Fe 3 o 4 The nanoclusters were washed three times with ethanol and water, and dissolved in Tris buffer;

[0046] (2) the Fe-containing material prepared in step (1) 3 o 4 Adjust the pH of the nanocluster Tris buffer to 8.5, slowly add 0.03M dopamine solution, react at room temperature for 4.5h until the color turns black, centrifuge and wash with deionized water for 3 times, and vacuum-dry the precipitate at 55°C to obtain dopa...

Embodiment 3

[0051] Include the following steps:

[0052] (1) 2mmol of FeCl 3 Dissolve in a mixed solution of 4mL ethylene glycol and 16mL monocarboxydiethylene glycol, stir for 1h, add 2g of polyvinylpyrrolidone to the above solution, heat the solution to 120°C to obtain a transparent yellow solution, stop heating after 2h And add 1.5g of sodium acetate, continue to stir for 1h, transfer the solution to a 25mL reactor, seal the reactor and heat it to 200°C, continue the reaction for 16h, stop heating and cool the reactor to room temperature, the obtained Fe 3 o 4 The nanoclusters were washed three times with ethanol and water, and dissolved in Tris buffer;

[0053] (2) the Fe-containing material prepared in step (1) 3 o 4 Adjust the pH of the nanocluster Tris buffer to 8.5, slowly add 0.03M dopamine solution, react at room temperature for 6 hours until the color turns black, centrifuge, wash with deionized water 3 times, and vacuum-dry the precipitate at 60°C to obtain dopamine-coated...

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Abstract

The invention provides a targeted adriamycin-loaded magnetic nanoparticles and a preparation method and application, and belongs to the magnetic nanoparticles and preparation thereof. The targeted adriamycin-loaded magnetic nanoparticles are formed by taking the magnetic Fe3O4 nanoparticles formed by using dopamine to wrap as a core, and an EGFR antibody covalently coupled on the surface of the magnetic Fe3O4 nanoparticles through 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride or N-hydroxysuccinimide, and adriamycin non-covalently coupled to the surface of the nanoparticles. The nanoparticles provided by the invention have good target recognition capacity to the colon cancer cell DLD-1, can effectively enter the internal of the DLD-1 cell, has excellent photo-thermal killing ability on the DLD-1 cell, can be used for the photo-thermal treatment and medicine chemotherapy of the colon cancer cell, and has a good magnetic resonance imaging function.

Description

technical field [0001] The invention relates to a modified magnetic nanoparticle Fe 3 o 4 Preparation of @PDA-PEG-EGFR-DOX NPs and their application in antitumor photothermal therapy. Background technique [0002] Colon cancer is one of the common clinical malignant tumors. In my country, the incidence of colon cancer is increasing year by year. At present, the clinical treatment for colon cancer is mainly surgical treatment, supplemented by radiotherapy, traditional Chinese medicine treatment and chemotherapy. Surgical resection of tumor tissue is an effective treatment, but if the resection of tumor tissue is insufficient, there will be residual tumor cells at the surgical margin, which will lead to tumor recurrence. Chemotherapy is currently the most important adjuvant therapy for the clinical treatment of colon cancer and the prevention of tumor recurrence and metastasis, and it has an important and positive therapeutic effect on all stages of colon cancer. [0003]...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/16A61K49/12A61K41/00A61K47/68A61K47/69A61K31/704A61P35/00
CPCA61K31/704A61K41/0052A61K49/12A61K49/16A61K49/1857A61K49/1875
Inventor 姜金兰牟旭鹏张福强
Owner JILIN UNIV
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