Application of kukoamine in preparing medicine for treating type II diabetes mellitus
A technology of stegosine and diabetes drug, which is used in drug combination, metabolic diseases, pharmaceutical formulations, etc.
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Embodiment 1
[0031] Determination of DPP-4 inhibitory activity
[0032] DPP-4 inhibitor screening method was used to make diocetin into a solution with a concentration of 1 mmol / L; and the positive drug dipeptide A to be made into a solution with a concentration of 1 mmol / L. Take a 96-well plate, add 50 μmol / L specific DPP-4 enzyme activity detection fluorescent probe 2-[4-(1,2,2-triphenylethylene)phenoxy]-acetic acid-lysine-phenylpropanoid Amino acid-proline-glutamic acid (TPE-KFPE) 20 μL, dichotomin solution 5 μL, 0.1 U / mL DPP-4 enzyme solution 5 μL, after mixing, incubate at 37°C for 30 min, at an excitation wavelength of 320 nm, The fluorescence intensity value was measured at an emission wavelength of 450nm. Calculate the inhibitory rate of DPP-4 activity by diocetin. Among them, enzyme solution, sample solution and reaction substrate solution are all prepared with 0.5mmol / L, pH 7.0 HEPES buffer solution.
[0033]
[0034] I 给药组 : Contains substrate and enzyme, and the fluoresc...
Embodiment 2
[0040] Chinese medicine active compound preparation
[0041] Mix 0.5 g of the active compound of traditional Chinese medicine in Example 1 with 10.5 g of polyethylene glycol-6000, heat and melt, and transfer the material to a dripping tank, drop the drug solution into liquid paraffin at 6-8°C, and remove the oil. , made 400 dropping pills.
Embodiment 3
[0043] Chinese medicine active compound preparation
[0044] Take 0.5g of the active traditional Chinese medicine compound of Example 1, 4.5g of glucose, 0.9g of sodium thiosulfate and 1L of distilled water, mix the above components evenly, freeze-dry, and pack in 500 tubes to obtain the product.
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