Efficient virus-free tissue culture method of grosvenor momordica fruit

Abstract

The invention discloses an efficient virus-free tissue culture method of grosvenor momordica fruit, the method is as follows: explants are sterilized to obtain sterile explants; the sterile explants are inoculated in a MS induction culture medium for induction culture to obtain sterile plantlets; the sterile plantlets are precultured in a MS pre culture medium to obtain pre culture plantlets; the pre cultured plantlets are inoculated to a MS virus-free culture medium for virus-free culture to obtain tube multiple shoots; strong tube multiple shoots are selected and inoculated to a 1 / 2MS rooting medium for rooting culture to obtain plants with roots; and finally the plants with roots are hardened and transplanted, wherein the MS induction culture medium comprises at least 0.5-1.0mg / L of Adamantanone, and the MS pre culture medium contains at least 0.5-1.0mg / L of Adamantanone and 0.1-0.6mg / L of silver nitrate. The proliferation coefficient of the multiple shoots of the grosvenor momordica fruit obtained by the method reaches 20 or 30 times, and the multiple shoots are strong. After inoculation to the rooting medium, the roots are easy to grow, and the rooting rate can reach 95%, and the survival rate is 95%.

Description

technical field [0001] The invention relates to the field of plant propagation of grosvenoria grosvenori, in particular to a tissue culture method for efficient detoxification of grosvenoria grosvenori. [0002] technical background [0003] Luo Han Guo (Siraitiagrosvenorii) is one of my country's traditional export commodities. It is sweet in taste and cool in nature. Laxative effect. It is one of the first batch of medicinal and edible materials approved by the country. It has a medicinal history of more than 300 years in Guangxi folks and is known as the "Oriental Divine Fruit". [0004] For a long time, Luo Han Guo has mainly been propagated by pressing vines in the planting process, resulting in species degradation, serious pest damage and other problems, especially viral diseases, the incidence rate of which is as high as 100% in serious plots, causing huge economic losses. In recent years, tissue cultured seedlings have been mainly used in the production of Luo Han Gu...

AI Technical Summary

Problems solved by technology

[0004] For a long time, Luo Han Guo has been mainly propagated by pressing vines in the planting process, resulting in species degradation, serious pest damage and other problems, especially viral diseases, the incidence rate of which is as high as 100% in serious plots, causing huge economic losses

In recent years, tissue cultured seedlings have been mainly used in the production of Luo Han Guo for propagation, but practice has shown that most of the tissue cultured seedlings sold on the market have latent viral diseases, resulting in outbreaks of viral diseases in field planting, seriously affecting yield and quality (Lin Wei et al., Guangxi Investigation on the occurrence of Luo Han Guo virus disease. Plant Protection, 2003.29(4):27-30; Tang Xuejun and Sun Guichun, Comprehensive management of Luo Han Guo virus disease. Journal of Guangxi Agricultural Sciences, 2006.21(5):43-44; Liang Huiling, Jiang Shuiyuan and Li Hong, Luo Han Guo Investigation on the Epidemiological Factors of Viral Diseases in Tissue Cultured Vaccines. Research on Special Products, 2008.30(2):50-52)

The invention patent with the application number 201510290985.0 discloses a method for the propagation of Luo Han Guo tissue culture seedlings, which selects healthy Luo Han Guo plants as the propagation material, and does not specifically target the plants containing viruses. The propagation material is simply sterilized, although it has achieved a certain effect, but it still needs to be improved, and the medium components used are relatively common, and it cannot be further sterilized during cultivation, so as to truly prevent the outbreak of viral diseases