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Technological method for extracting lumbrokinase by utilizing living earthworms

A technology of living earthworms and process methods, applied in the directions of biochemical equipment and methods, enzymes, peptidases, etc., can solve the problems of low utilization rate of chemical fertilizers in the season, increasing farmers' input, and low utilization rate of chemical fertilizers.

Inactive Publication Date: 2017-05-24
天津天丰泽田生物科技有限公司
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  • Abstract
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Problems solved by technology

[0002] my country is a big fertilizer application country, but the seasonal utilization rate of chemical fertilizers is very low
At present, my country's nitrogen fertilizer utilization rate is about 30% to 35%, and the chemical fertilizer utilization rate is low, which not only wastes resources, but also increases farmers' investment
It also causes environmental pollution, and the discharge of phosphogypsum, high-phosphorus wastewater and high-fluorine-containing gases has also brought great pressure to the environment.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0015] The process for extracting lumbrokinase from live earthworms of the present invention will be specifically described below in conjunction with examples.

[0016] Collection of earthworms: collect the live earthworms delivered on the same day, after removing the soil, wash them gently with warm water to clean the surface of the earthworms.

[0017] Extraction of lumbrokinase: Weigh 100kg of washed earthworms, spread a thin layer on the extraction bed (mesh structure), and spray the mobile spray head above the extraction bed on the extraction bed every 5-10 minutes to stimulate the body secretion of earthworms The substance of lumbrokinase until the earthworm is completely dead.

[0018] Precipitation: Take out the extract solution under the extraction bed, add protein precipitant, and let stand.

[0019] Centrifugation: centrifuge the extract containing precipitate obtained in the above steps in a high-speed centrifuge with a rotation speed of 10000r / s for 10-15min.

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Abstract

The invention provides a technological method for extracting lumbrokinase by utilizing living earthworms. The technological method comprises the following technological steps: collecting the earthworms, extracting the lumbrokinase, precipitating, centrifuging, sterilizing and drying and pulverizing. The extracted lumbrokinase can be used for promoting the increasing of the quantity of the earthworms and microorganisms in soil; meanwhile, the soil can be effectively activated and loosened and the content of organic matters in the soil is increased; a soil structure and physicochemical properties are improved, and absorption of root systems on nutrients is facilitated.

Description

technical field [0001] The invention relates to a process method for extracting lumbrokinase by using live earthworms. Background technique [0002] my country is a big country in the application of fertilizers, but the seasonal utilization rate of chemical fertilizers is very low. At present, the utilization rate of nitrogen fertilizer in my country is about 30% to 35%, and the utilization rate of chemical fertilizer is low, which not only wastes resources, but also increases farmers' investment. It also causes environmental pollution, and the discharge of phosphogypsum, high-phosphorus wastewater and high-fluorine-containing gases has also brought great pressure to the environment. The second is resource pressure. Resources for producing fertilizers, such as phosphate rock, potash, coal and natural gas, are non-renewable resources. How to save resources and meet the growing demand for fertilizers is a big challenge for China's fertilizer industry. [0003] Lumbrokinase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/68
CPCC12N9/6435C12Y304/21007
Inventor 张见高雪燕赵娟
Owner 天津天丰泽田生物科技有限公司
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