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Immobilized preparation and application of algicidal bacterium microspheres

An algae-lysing bacteria and immobilization technology, applied in biochemical equipment and methods, bacteria, applications, etc., can solve the problem of being easily eaten by other organisms, difficult to grow or kill algae by algae-lysing bacteria, and easy to be eaten by free algae-lytic cells. Problems such as water flushing away, to achieve the effect of inhibiting the overgrowth of algae, controlling the overgrowth of algae, and maintaining activity

Inactive Publication Date: 2017-05-24
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, algicidal bacteria are mainly used in the form of free cells, but free algicidal cells are easily washed away by water flow under running water conditions, and easily eaten by other organisms under static water conditions. Due to the lack of carriers for bacterial attachment and growth and reproduction, It is difficult for algicidal bacteria to perform the function of inhibiting algae growth or killing algae stably for a long time

Method used

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  • Immobilized preparation and application of algicidal bacterium microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Prepare a sterile liquid medium, the composition is: 5g sodium chloride, 10g peptone, 5g beef extract, adjust the pH to 7.2 with 1mol / L sodium hydroxide or 1mol / L hydrochloric acid solution, and sterilize at 120°C and 110kPa Bacteria 30min.

[0039] (2) The alginolytic bacterium Brevundimonas diminuta ( Brevundimonas diminuta , CGMCC No.11571) were inoculated in the solution of step (1), and cultured at 37°C for 72 hours to obtain a culture solution.

[0040] (3) Centrifuge the cultured bacterial solution in step (2) at 4800r / min for 10 min, and resuspend the obtained bacterial cells in the culture medium in step (1) with 40% of the original sampling volume.

[0041] (4) Prepare a polyvinyl alcohol solution with a mass fraction of 10%, the mass concentration of sodium alginate is 0.25%, and prepare it in distilled water. First add polyvinyl alcohol, then sodium alginate, dissolve at 86°C, and keep the solution The final temperature was 35°C.

[0042] (5) Dissolve...

Embodiment 2

[0046] (1) Prepare a sterile liquid medium, the composition is: 5g sodium chloride, 10g peptone, 5g beef extract, adjust the pH to 7.3 with 1mol / L sodium hydroxide or 1mol / L hydrochloric acid solution, and sterilize at 120°C and 110kPa Bacteria 30min.

[0047] (2) The alginolytic bacterium Brevundimonas diminuta ( Brevundimonas diminuta , CGMCC No.11571) were inoculated in the solution of step (1), and cultured at 37°C for 80 hours to obtain a culture solution.

[0048] (3) Centrifuge the cultured bacterial solution in step (2) at 4800r / min for 10 min, and resuspend the obtained bacterial cells in the culture medium in step (1) with 50% of the original sampling volume.

[0049] (4) Prepare a polyvinyl alcohol solution with a mass fraction of 11%, the mass concentration of sodium alginate is 0.50%, and prepare it in distilled water. First add polyvinyl alcohol, then sodium alginate, dissolve at 88°C, and keep the solution The final temperature was 37°C.

[0050] (5) Dissolve...

Embodiment 3

[0054] (1) Prepare a sterile liquid medium, the composition is: 5g of sodium chloride, 10g of peptone, 5g of beef extract, adjust the pH to 7.5 with 1mol / L sodium hydroxide or 1mol / L hydrochloric acid solution, and sterilize at 120°C and 110kPa. Bacteria 30min.

[0055] (2) The alginolytic bacterium Brevundimonas diminuta ( Brevundimonas diminuta , CGMCC No.11571) were inoculated in the solution of step (1), and cultured at 37°C for 90 hours to obtain a culture solution.

[0056] (3) Centrifuge the cultured bacterial solution in step (2) at 4800r / min for 10 min, and resuspend the obtained bacterial cells in the culture medium in step (1) with 60% of the original sampling volume.

[0057] (4) Prepare a polyvinyl alcohol solution with a mass fraction of 12%, the mass concentration of sodium alginate is 1.0%, and prepare it in distilled water. First add polyvinyl alcohol, then add sodium alginate, dissolve at 90°C, and keep the solution The final temperature was 37°C.

[0058]...

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Abstract

The invention relates to the technical field of water environment and ecology restoration, in particular to immobilized preparation and application of algicidal bacterium microspheres. Bacterial cells in cultured algicidal bacterium liquid are separated and suspended in a beef extract peptone liquid culture medium 20-80% of original sample volume, polyvinyl alcohol and sodium alginate are selected as embedding bodies, a saturated boric acid solution of calcium chloride serves as a cross-linking agent, and immobilized cross-linking is performed at the temperature of 4 DEG C for 16-32 hours to prepare the algicidal bacterium microspheres. In addition, the invention discloses application of the algicidal bacterium microspheres in inhibiting of alga overgrowth, repeated adding of algicidal bacteria is avoided, and disposable addition of the algicidal bacteria is achieved. A nutrient substance adding mode is improved, the algicidal bacterium inhibiting effect of the algicidal bacteria is not affected, and water quality influence is also not brought. The preparation process is simple, the cost is low, large-scale popularization is easy, secondary pollution is not produced, and the algicidal bacterium microspheres provide an effective way for water bloom treatment.

Description

technical field [0001] The invention relates to the technical field of ecological restoration of water environment, in particular to the preparation and application of immobilized algae-dissolving bacterial microspheres. Background technique [0002] Due to human activities, industrial wastewater containing nitrogen, phosphorus and other nutrients, farmland drainage and domestic sewage enter rivers and lakes in large quantities, resulting in the increase of nitrogen, phosphorus and other nutrients in the water, and the explosive growth of algae in the water. , landscape effects and even adverse effects on human health. [0003] There are mainly physical methods, chemical methods and biological methods to control the overgrowth of algae in water bodies. Physical methods include manual salvage, ultrasonic algae removal, flocculant dosing (polyaluminum chloride, polysilicon aluminum sulfate, phosphate clay), etc.; chemical methods refer to directly adding algicides to water bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/08C12N11/10A01N63/00A01P13/00C12R1/01C12R1/05
CPCA01N63/00C12N1/20C12N11/08C12N11/10
Inventor 梁文艳张恒峰王媛媛樊乾龙
Owner BEIJING FORESTRY UNIVERSITY
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