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Straw decomposing additive for decomposition in low-temperature state and preparation method of straw decomposing additive

A technology of straw decomposing agent and state, which is applied in the field of straw decomposing agent and its preparation, can solve the problems of slow decomposing agent decomposing and incomplete decomposing, and achieve the effect of improving enzyme activity and good decomposing effect

Active Publication Date: 2017-05-24
FUZHOU SANHEYUAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of slow start-up and incomplete decomposition of decomposing agents in the above-mentioned prior art, the present invention provides a decomposing agent that can quickly start decomposing at low temperature (below 10°C)

Method used

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  • Straw decomposing additive for decomposition in low-temperature state and preparation method of straw decomposing additive

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Effect test

Embodiment 1

[0024] The decomposing corn stalks were enriched and cultured at low temperature (5°C), and the infusion solution was diluted, and then an appropriate dilution was selected to spread on a double-layer flat plate with cellulose as the only carbon source and cultured at low temperature (5°C) until a single colony was formed, and then punctured. Pick the colonies to obtain the low-temperature decomposing bacteria. It was identified as the Arthrobacter strain YO-H with the preservation number "CGMCC NO.4119".

[0025] Culture the strain YO-H, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, and Trichoderma harzianum separately until the bacterial concentration is 10 8 / mL, mixed evenly at a volume ratio of 1:1:1:2:2, inoculated into wheat bran with 50% water content at a ratio of 1:10 and cultivated for 10 days, and air-dried in a cool place until the mass fraction of water in the wheat bran was small It is equal to 25%, adding 1%--1.5% cellulase, mixing evenly to ...

Embodiment 2

[0032] The use effect of embodiment 2 decomposing agent of the present invention

[0033] How to use: Organic materials are composted layer by layer. Every layer is 15-20 centimeters, spread evenly layer by layer the decomposing agent of embodiment 1 of the present invention, urea or human and animal excrement and urine, dosage: decomposing agent 2‰; Urea 6-8kg / ton or human and animal excrement and urine 100-200kg / ton, adjust Moisture to 60-70%.

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Abstract

The invention relates to a compound microbial agent and a preparation method thereof. The problems that a decomposing agent in the prior art is slow in decomposition start and incomplete in decomposition are solved. A straw decomposing additive for decomposition in a low-temperature state is prepared from a strain YO-H, bacillus subtilis, saccharomyces cerevisiae, Aspergillus niger and Trichoderma harzianum. The straw decomposing additive has the advantages that the strain YO-H is used for the straw decomposing additive, and decomposition can be rapidly started even when the environment temperature is low; the strain YO-H and other decomposing bacteria are matched, and the decomposition effect is better; an additional enzymic preparation is added, cellulase enzyme activity of the decomposing additive is improved, about 3-4 days is available for utilizing the additionally-added enzymic preparation for decomposing lignin and cellulose before the decomposing bacteria massively propagate to generate new enzymes, and in other words, the decomposing time is shortened by 3-4 days.

Description

[0001] This case is a divisional application based on the invention patent with the application date of 2013-12-03, the application number of 201310641595.4, and the title of "a straw decomposing agent for rapid decomposing at low temperature and its preparation method" as the parent case. technical field [0002] The invention relates to a straw decomposing agent and a preparation method thereof, in particular to a straw decomposing agent for decomposing at low temperature and a preparation method thereof. Background technique [0003] Organic materials such as straw contain a large amount of lignin, which surrounds hemicellulose and hemicellulose and fills between microfibrils, and is tightly combined with cell wall components. "Prime" constitutes a whole, which increases the difficulty of microbial decomposition of straw. The few days before straw decomposing is the growth and reproduction period of microorganisms in decomposing agent, which contribute little to decomposi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05G1/00C05F15/00C05F11/08C12R1/06C12R1/125C12R1/865C12R1/685C12R1/885
Inventor 杜秉海林金新董少英姚良同丁延芹
Owner FUZHOU SANHEYUAN BIOTECHNOLOGY CO LTD
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