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Method for repairing sediment pollution in open sea area

A sea area sediment and repair method technology, applied in the field of water environment treatment by microorganisms, can solve the problems of few demonstrations and projects, lack of open sea sediment pollution microbial repair methods and applications, etc., to reduce workload and working hours, increase The effect of pollutant degradation and the effect of ensuring the repair effect

Active Publication Date: 2017-05-24
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, microbial remediation of oil pollution in marine sediments is mostly at the level of theoretical research and small-scale laboratory simulation of marine environment experiments, and there are few demonstrations and projects of large-scale microbial remediation of oil pollution in offshore sediments.
On the one hand, due to the special marine environment, offshore construction has strict requirements on weather, sea conditions, construction vessels and offshore operating experience. Now most of the organic pollution in the ocean, especially oil pollution remediation, engineering demonstrations are mainly concentrated in tidal flat areas, lacking open sea areas Microbial remediation method and application of sediment pollution

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  • Method for repairing sediment pollution in open sea area
  • Method for repairing sediment pollution in open sea area
  • Method for repairing sediment pollution in open sea area

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Screening and combination of efficient petroleum degrading bacteria

[0050] Using the microorganisms in the sediments of the Yantai sea area as the source of highly efficient oil-degrading bacteria, the indigenous strains with good degradation effects on oil pollution were screened, and the bacterial liquid was mixed according to the ratio of the maximum degradation effect, adsorbed on the surface of zeolite, and wrapped with a coating agent, and finally Carry out subcontracting and release, and complete the bioremediation of oil-polluted sediments in designated sea areas of the Bohai Sea.

[0051] (1) Strain screening: Weigh 2g of petroleum-contaminated seawater sediment samples and add them to Erlenmeyer flasks filled with seawater culture medium and freshwater culture medium respectively, carry out shaking culture on a constant temperature shaker at 30°C and 180r / min for 3 days, and then pipette Insert 1 mL of the culture solution into the screening medium...

Embodiment 2

[0064] Example 2 Sea Area Sediment Oil Contaminated Microbial Restoration

[0065]Remediation of oil pollution in sediments in the Bohai Sea.

[0066] (1) Restoration strains: Acinetobacter calcoacetate, Pseudomonas putida and Bacillus shamford.

[0067] (2) Fermentation and mixing of bacterial strains: the three bacterial strains are respectively subjected to large-scale fermentation, and the respective concentration of the bacterial liquid after fermentation is 2-3OD. After fermentation, Acinetobacter calcoacetate, Pseudomonas putida, and Bacillus spp. were mixed according to the ratio of the number of colonies of the strains to 3:2:4. 9 CFU / ml, mix and store in a refrigerator at 4 degrees Celsius.

[0068] (3) Adsorption and wrapping of bacterial liquid: the mixed bacterial liquid is mixed and adsorbed according to the mass ratio of bacterial liquid and zeolite at 2:1, and the adsorption time is 2 hours. After the water content is 60%-70%, spray 7wt% polyglutamic acid so...

Embodiment 3

[0080] Microbial remediation of furazolidone antibiotic pollution in embodiment 3 seawater

[0081] The combined bacterial agent of Acinetobacter calcoacetate, Pseudomonas putida and Bacillus suffolus has a good degradation effect on furazolidone antibiotics in seawater.

[0082] (1) Microbial strains: Acinetobacter calcium acetate, Pseudomonas putida, Bacillus shamford;

[0083] (2) Combination of bacterial strains: According to the three-level three-factor orthogonal test, the concentration ratio of Acinetobacter calcoaceticus, Pseudomonas putida, and Bacillus shamford was mixed in a concentration ratio of 2:2:1, and finally The total bacterial count is 1×10 8 Inoculate in the inorganic salt peptone liquid medium containing 500ppm furazolidone for cultivation, and use the inorganic salt peptone liquid medium containing furazolidone without inoculation of the degradation strain as a control. At different time points, 500ul of the culture solution was taken out, centrifuged ...

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Abstract

The invention relates to the field of water environment treatment conducted by microorganisms, in particular to a method for repairing sediment pollution in an open sea area or a deep freshwater area, a river mouth and the like. The method comprises the steps: loading a bacterial strain with zeolite, coating with a flocculating agent and putting the coated bacterial agent into a to-be-treated water area so as to repair sediment petroleum and organic pollutants in the water area. The method mainly aims at the seawater / freshwater area with the water depth being 2 to 50 meters and has a good effect of repairing the sediment petroleum pollution and other organic pollution. The method is integral in system and simple in operating method, has the remarkable effect of repairing the sediment, no secondary pollution and high offshore construction safety, and is particularly suitable the special-function sea areas such as a petroleum exploitation area and an aquaculture area, and can guide implementation of sea sediment pollution repair engineering.

Description

technical field [0001] The invention relates to the field of water environment treatment by using microorganisms, in particular, the invention relates to a repair method for sediment pollution in open sea areas or deep fresh water areas, estuaries and the like. Background technique [0002] Marine organic pollution, especially marine oil spills and oil pollution has always been an important issue affecting the marine environment and marine ecology. At present, the emergency treatment of marine oil pollution, especially sudden oil spills, is mainly aimed at the initial stage of oil spills. The surface seawater of the ocean is treated with physical interception, recycling, and chemical dispersants for emergency treatment. However, there are no good physical and chemical treatment methods for seabed oil spills or oil pollution that sinks into seabed sediments with water movement. Environmental self-purification of oil-degrading microorganisms in sediments and artificial introdu...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/08
CPCC02F3/341C02F3/348C02F2103/08C02F2305/06
Inventor 陈令新王巧宁付龙文盛彦清吕敏
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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