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Application of lnc (long non-coding) RNA lnc-DIF

A long-chain non-coding and RNA interference technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., and can solve problems such as diagnosis and treatment that have not yet been studied and reported

Inactive Publication Date: 2017-05-17
NORTHWESTERN POLYTECHNICAL UNIV
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Problems solved by technology

[0005] As a lncRNA, lnc-DIF is located on mouse chromosome 19 chr19: 38207571-38208896, Genebank ID AK138929.1, 1326bp long, first discovered in 1999 (Carninci, P. & Hayashizaki, Y. High-efficiency full-length cDNA cloning, JOURNAL Meth.Enzymol.1999(303),19-44), for its role in osteoblast differentiation and bone formation, and its role in the diagnosis and treatment of skeletal diseases such as human osteoporosis The action energy has not yet been studied and reported

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  • Application of lnc (long non-coding) RNA lnc-DIF

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Embodiment Construction

[0025] refer to figure 2 : Using qPCR technology, it was found that lnc-DIF was highly expressed in the bone tissue samples of the aged osteoporosis mouse model and the HLU hindlimb unloaded osteoporosis mouse model.

[0026] (1) Design and prepare specific primers for lnc-DIF, the sequences are shown in Table 1;

[0027] (2) Establish an aging mouse model of osteoporosis:

[0028] C57BL / 6 experimental mice were selected and raised to 15 and 18 months old. The mice were sacrificed and tibia samples were collected.

[0029] (3) Establish HLU hindlimb unloading osteoporosis mouse model:

[0030] The C57BL / 6 experimental mice were selected, and the mice were hung on the rings of the tail suspension device with a paper clip, and the mouse spine was 30° from the ground to carry out tail suspension and hindlimb unloading. During the experiment, the animals were allowed to eat and drink freely. After 28 days, the mice were sacrificed, and tibia samples were collected.

[0031] (...

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Abstract

The invention discloses an application of lnc (long non-coding) RNA lnc-DIF. The technical scheme is that a femur sample of an old osteoporotic mouse model and a femur sample of a hindlimb-unloaded osteoporotic mouse model are detected firstly, and high expression of the lnc RNA lnc-DIF in bone tissue of the old and hindlimb-unloaded osteoporotic mouse models is discovered. An RNA interference sequence of the lnc RNA lnc-DIF is further designed and prepared, osteogenic differentiation function experiments discover that the lnc RNA lnc-DIF inhibits osteogenic cell differentiation and can be specifically bound to osteogenesis related miR-489-3p. Accordingly, the lnc RNA lnc-DIF can be used for preparing drugs for treating osteoporosis caused by various factors as well as other diseases of a skeletal system.

Description

technical field [0001] The invention belongs to the field of molecular biomedicine, in particular to the application of a long-chain non-coding RNA lnc-DIF. Background technique [0002] Non-coding RNA (Non-coding RNA) refers to RNA that does not code for protein. These include microRNA (miRNA) and long non-coding RNA (long non-coding RNA, lncRNA for short). The common feature of these RNAs is that they can be transcribed from the genome, but do not encode proteins, and perform their respective biological functions at the RNA level. Among them, lncRNA is a kind of eukaryotic endogenous RNA longer than 200nt, widely distributed in eukaryotic organisms, and plays an important regulatory role in various physiological and pathological processes such as cell differentiation, organ development and disease occurrence. Osteoblasts are the main functional cells for bone formation. As people age, bone development also undergoes dynamic changes, in which bone formation guided by ost...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 骞爱荣印崇苗治平张琰胡丽芳陈志浩赵帆李迪杰马剑华苏佩红马小莉仇伍霞杨超飞李思宇王牌
Owner NORTHWESTERN POLYTECHNICAL UNIV
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