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Magnetic bead composition for improving nucleic acid extraction rate and application of magnetic bead composition

A composition and magnetic bead technology, applied in recombinant DNA technology, DNA preparation and other directions, can solve the problems affecting the binding efficiency of magnetic bead solution and nucleic acid, incomplete cleaning, unsatisfactory purification effect, etc., and achieve rapid adsorption and desorption. , The effect of maintaining nucleic acid stability and improving binding efficiency

Active Publication Date: 2017-05-17
广州奇辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these multi-component lysates will affect the binding efficiency of the magnetic bead solution and nucleic acid, and the follow-up cleaning is not thorough, and some small amounts of impurities (such as protein, polysaccharide, fat, etc.) will remain, and the purification effect is not ideal. The recovery rate of trace nucleic acid is lower than 80%

Method used

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  • Magnetic bead composition for improving nucleic acid extraction rate and application of magnetic bead composition
  • Magnetic bead composition for improving nucleic acid extraction rate and application of magnetic bead composition
  • Magnetic bead composition for improving nucleic acid extraction rate and application of magnetic bead composition

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Embodiment 1

[0041] Embodiment 1 A kind of magnetic bead composition that improves nucleic acid extraction rate

[0042] Composition and concentration:

[0043] 1mg / ml magnetic beads, 0.022mol / L polyethylene glycol 8000, 2.2mol / L sodium chloride, 0.5mol / L ethylenediaminetetraacetic acid solution, 10mol / L tris solution, ultrapure water quantity.

[0044] Preparation method:

[0045] Calculate the dosage of each component according to the total amount of configuration and the concentration of each component; prepare materials according to the calculation results, and use a ten-thousandth balance to weigh 1.76g of polyethylene glycol 8000, 1.287g of sodium chloride, and 1.46g of ethylenediaminetetraacetic acid. , 0.012g Tris in a 50mL clean beaker, add 5mL of ultrapure water and place the beaker on a 55°C water bath to aid in dissolution. After all solid dissolved solutions become clear, transfer the contents of the beaker to a 10mL volumetric flask , use a 1mL pipette to inhale clean ultr...

Embodiment 2

[0046] Embodiment 2 A kind of magnetic bead composition that improves nucleic acid extraction rate

[0047] Composition and concentration:

[0048]1mg / ml magnetic beads, 0.024mol / L polyethylene glycol 8000, 2.5mol / L sodium chloride, 0.5mol / L ethylenediaminetetraacetic acid solution, 10mol / L tris solution, ultrapure water quantity.

[0049] Preparation method:

[0050] Calculate the dosage of each component according to the total amount of configuration and the concentration of each component; prepare materials according to the calculation results, use a ten-thousandth balance to weigh 1.92g polyethylene glycol 8000, 1.5g sodium chloride, and 1.46g ethylenediaminetetraacetic acid , 0.012g Tris in a 50mL clean beaker, add 5mL of ultrapure water and place the beaker on a 55°C water bath to aid in dissolution. After all solid dissolved solutions become clear, transfer the contents of the beaker to a 10mL volumetric flask , use a 1mL pipette to inhale clean ultrapure water to cl...

Embodiment 3

[0051] Embodiment 3 A kind of magnetic bead composition that improves nucleic acid extraction rate

[0052] Composition and concentration:

[0053] 1mg / ml magnetic beads, 0.025mol / L polyethylene glycol 8000, 2.5mol / L sodium chloride, 1mol / L ethylenediaminetetraacetic acid solution, 15mol / L tris solution, ultrapure water balance .

[0054] Preparation method:

[0055] Calculate the dosage of each component according to the total amount of configuration and the concentration of each component; prepare materials according to the calculation results, prepare materials according to the calculation results, and use a ten-thousandth balance to weigh 2g polyethylene glycol 8000, 1.5g sodium chloride, and 2.92g ethylene glycol respectively. Diaminetetraacetic acid, 0.018g Tris in a clean 50mL beaker, add 5mL of ultrapure water, place the beaker on a water bath at 55°C to aid in dissolution, and transfer the contents of the beaker after all solid dissolved solutions become clear Tran...

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Abstract

The invention belongs to the field of molecular biology technologies, and particularly relates to a magnetic bead composition for improving a nucleic acid extraction rate and an application of the magnetic bead composition. The magnetic bead composition comprises the following components by concentration: nano magnetic beads of 1mg / mL, polyethylene glycol 8000 of 0.018-0.026mol / L, sodium chloride of 2.2-2.6mol / L, an ethylenediamine tetraacetic acid solution of 0.5-2mol / L, and a trihydroxymethyl aminomethane solution of 10-20mmol / L. The magnetic bead solution provided by the invention can strengthen the adsorption efficiency of the magnetic beads and nucleic acid and can greatly improve the extraction rate; and especially for extraction of a trace-amount nucleic acid sample, the magnetic bead solution is more obvious in advantage, and reduces the operation time.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a magnetic bead composition for improving nucleic acid extraction rate and application thereof. Background technique [0002] Superparamagnetic nano-magnetic beads are a new type of functional material first developed by Senyei A E in 1978. Since its development, it has been widely used in different fields such as cell sorting, protein purification, nucleic acid extraction, bacterial detection, and immune analysis. Among them, in the field of nucleic acid extraction, superparamagnetic nano-magnetic beads have realized the combination of biological science and nanomaterials, which is a major breakthrough in the innovation of nucleic acid extraction and purification technology. [0003] The particle size of superparamagnetic nano-magnetic beads is in the nanometer range, which can be quickly aggregated in the presence of a magnetic field, and can be uniformly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013
Inventor 朱奇李岩松罗李江刘丽戚国杰
Owner 广州奇辉生物科技有限公司
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