Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for characterizing microorganism phagocytosis function of macrophages of trachinotus ovatus

A technology of macrophages and microorganisms, applied in the field of cell biology, can solve the problems of isolation, purification, culture and characterization of macrophages in the kidney of pompano pomfret head that have not been seen

Active Publication Date: 2017-05-10
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are few reports on the immune function of S. ovata. The head kidney is an important immune organ of fish. There are no reports on the isolation, purification, culture and characterization of macrophages from the head kidney of S. ovata.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for characterizing microorganism phagocytosis function of macrophages of trachinotus ovatus
  • Method for characterizing microorganism phagocytosis function of macrophages of trachinotus ovatus
  • Method for characterizing microorganism phagocytosis function of macrophages of trachinotus ovatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0070] In order to better explain the technical solutions of the present invention, various embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

[0071] 1. Experimental materials

[0072] 1.1 Experimental fish

[0073] Oval pomfret, commonly known as golden pomfret. The body is pomfret-shaped, high and flat on the sides; the body length is 1.7-1.9 times the body height and 3.8 times the head length. The tail peduncle is short and thin, and the side is flat. Head small, taller than long. The occipital crest is prominent. The he...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
body lengthaaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for characterizing a microorganism phagocytosis function of macrophages of trachinotus ovatus. The trachinotus ovatus is dissected and separated, and the macrophages of the head kidney of the trachinotus ovatus are obtained and are extracted with a Percoll solution density gradient centrifugation method. The number of survived cells cultured in vitro is calculated with a trypan blue staining method, and the survival condition of the cells in one week is detected. The phagocytosis of the macrophages of the head kidney of the trachinotus ovatus on Nocardia seriolea, saccharomycetes and the like is determined through a phagocytosis experiment, so that the phagocytosis function of the macrophages is observed. An experimental result shows that the macrophages of the trachinotus ovatus can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the trachinotus ovatus can be observed very well. A foundation is laid for further research of physiology, pathology and toxicology of the macrophages of the trachinotus ovatus.

Description

technical field [0001] The invention belongs to the field of cell biology and relates to a method for characterizing the interaction between immune cells and microorganisms, in particular to a method for characterizing the phagocytosis of microorganisms by pomfret macrophages. Background technique [0002] Oval pomfret (Trachinotus ovatus) (Linnaeus), commonly known as golden pomfret, yellow wax pomfret, belongs to Osteichthyes, Vertebrate Phylum, Osteichthyes, Perciformes, Trevalidae, Pomfrets genus. Oval pomfret has a flat side, oval shape, large body, fast growth, and strong disease resistance. In natural waters, pompano pomfret prefers pomfret pomfret to forage and migrate in clusters, vying for food fiercely, mainly feeding on small molluscs and small snails. The large individual weighs 5-10kg, and the meat is tender and delicious. It is a valuable edible fish. [0003] Pomfret ovata is a seawater fish with strong disease resistance, and there were few reports on its ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N15/10G01N1/28
CPCG01N1/2813G01N15/10G01N33/5055G01N2015/1006
Inventor 夏立群鲁义善简纪常蔡佳
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com